FokI requires two specific DNA sites for cleavage

Journal of Molecular Biology

Volumn 309, Issue 1, 2001, Pages 69-78

  Vanamee, Éva Scheuring ^a   Santagata, Sandro ^a   Aggarwal, Aneel K ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

DNA recognition and cleavage; Restriction modification system; Specific and non specific DNA; Synaptic complex

Indexed keywords

DNA; METAL ION; RESTRICTION ENDONUCLEASE; RESTRICTION ENDONUCLEASE FOKI; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; DIMERIZATION; DNA CLEAVAGE; DNA PROTEIN COMPLEX; DNA SEQUENCE; GEL FILTRATION; LIGHT SCATTERING; PRIORITY JOURNAL; REACTION ANALYSIS; ULTRACENTRIFUGATION;

EID: 0035946911     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4635     Document Type: Article

References (34)

1. New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI)
2. Cleaving DNA at any predetermined site with adapter primers and class-IIs restriction enzymes
3. Insertion and deletion mutants of FokI restriction endonuclease
4. Hybrid restriction enzymes: Zinc finger fusions to FokI cleavage domain
5. Site-specific cleavage of DNA-RNA hybrids by zinc finger/FokI cleavage domain fusions
6. Alteration of the cleavage distance of FokI restriction endonuclease by insertion mutagenesis
7. A detailed study of the substrate specificity of a chimeric restriction enzyme
8. Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains
9. Structure of the multimodular endonuclease FokI bound to DNA
10. Structure of the restriction endonuclease FokI suggests a two-site DNA cleavage mechanism
11. C-terminal deletion mutants of the FokI restriction endonuclease
12. Single amino acid substitutions uncouple the DNA binding and strand scission activities of FokI endonuclease
13. DNA binding mode of class-IIs restriction endonuclease FokI revealed by DNA footprinting analysis
14. Structure of restriction endonuclease BamHI and its relationship to EcoRI
15. Structure of restriction endonuclease BamHI phased at 1.95 Å resolution by MAD analysis
16. FokI dimerization is required for DNA cleavage
17. Restriction endonucleases requiring two recognition sites
18. EcoRII can be activated to cleave refractory DNA recognition sites
19. Formation of a cleavasome: Enhancer DNA-2 stabilizes an active conformation of NaeI dimer
20. Restriction endonucleases functionally interacting with two DNA sites
21. Imaging DNA loops induced by restriction endonuclease EcoRII. A single amino acid substitution uncouples target recognition from cooperative DNA interaction and cleavage
22. The SfiI restriction endonuclease makes a four-stranded DNA break at two copies of its recognition sequence
23. Reactions of type II restriction endonucleases with 8-base pair recognition sites
24. The Cfr10I restriction enzyme is functional as a tetramer
25. Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA
26. Overproduction and crystallization of FokI restriction endonuclease
27. Purification and characterization of the FokI restriction endonuclease
28. Divalent metal ions at the active sites of the EcoRV and EcoRI restriction endonucleases
29. Assembly of a 12/23 paired signal complex: A critical control point in V(D)J recombination
30. Structure of BamHI bound to nonspecific DNA: A model for DNA sliding
31. Structure of BamHI endonuclease bound to DNA: Partial folding and unfolding on DNA binding
32. Changing endonuclease EcoRII Tyr308 to Phe abolishes cleavage but not recognition: Possible homology with the Int-family of recombinases
33. DNA topoisomerase and recombinase activities in NaeI restriction endonuclease
34. Unexpected structural diversity in DNA recombination: The restriction endonuclease connection