Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection

Methods in Molecular Biology

Volumn 1123, Issue , 2014, Pages 97-104

  Wolfs, Jason M ^a   Kleinstiver, Benjamin P ^a   Edgell, David R ^a  

^a WESTERN UNIVERSITY   (Canada)

Author keywords

DNA gyrase toxin; DNA target sequence preference; Homing endonuclease; Two plasmid genetic selection

Indexed keywords

CHLORAMPHENICOL; DNA GYRASE TOXIN; DNA TOPOISOMERASE (ATP HYDROLYSING); ENDONUCLEASE; KANAMYCIN; TOXIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA CLEAVAGE; DNA LIBRARY; DNA SCREENING; DNA SEQUENCE; ESCHERICHIA COLI; GENE TARGETING; GENETIC SELECTION; HEAT SHOCK; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BINDING SITES; DNA CLEAVAGE; ENDONUCLEASES; GENE LIBRARY; GENE TARGETING; PLASMIDS; SUBSTRATE SPECIFICITY;

EID: 84934444305     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-968-0_8     Document Type: Article

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