Production of prone-to-aggregate proteins

FEBS Letters

Volumn 588, Issue 2, 2014, Pages 236-246

  Lebendiker, Mario ^a   Danieli, Tsafi ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Aggregation analysis; Aggregation suppressor; Buffer condition; Chaotrope; Chaperone; Fusion protein; Induction condition; Intrinsically disordered protein; Intrinsically disordered region; Kosmotrope; Protein aggregation; Protein concentration; Protein storage; Stabilizer

Indexed keywords

BUFFER; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; NUTILIZATION SUBSTANCE A; RECOMBINANT PROTEIN; RNA POLYMERASE; SODIUM CHLORIDE; SUMO PROTEIN; SURFACTANT; T7 RNA POLYMERASE; THIOREDOXIN; UNCLASSIFIED DRUG;

CIRCULAR DICHROISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; DE3 GENE; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE; HIGH THROUGHPUT SCREENING; IONIC STRENGTH; LIGHT SCATTERING; LOW TEMPERATURE; MECHANICAL STRESS; MOLECULAR CLONING; MONITORING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN SYNTHESIS; REVIEW; STORAGE; ULTRACENTRIFUGATION;

ESCHERICHIA COLI;

AGGREGATION ANALYSIS; AGGREGATION SUPPRESSOR; BUFFER CONDITION; CHAOTROPE; CHAPERONE; FUSION PROTEIN; INDUCTION CONDITION; INTRINSICALLY DISORDERED PROTEIN; INTRINSICALLY DISORDERED REGION; KOSMOTROPE; PROTEIN AGGREGATION; PROTEIN CONCENTRATION; PROTEIN STORAGE; STABILIZER;

GENETIC ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS;

EID: 84892367365     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2013.10.044     Document Type: Review

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