Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Leibly, David J ^a,b   Nguyen, Trang Nhu ^a,b   Kao, Louis T ^a,b   Hewitt, Stephen N ^a,b   Barrett, Lynn K ^a,b   van Voorhis, Wesley C ^a,b  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; BETAINE; BUFFER; CITRATE POTASSIUM; COPPER CHLORIDE; DETERGENT; LIGAND; MANNITOL; PROLINE; RECOMBINANT PROTEIN; SODIUM CHLORIDE; TREHALOSE; XYLITOL;

ARTICLE; CONTROLLED STUDY; CRYSTALLIZATION; CRYSTALLOGRAPHY; CYTOLYSIS; ENZYMOLOGY; ESCHERICHIA COLI; NONHUMAN; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN TARGETING; STRUCTURAL GENOMICS; TOXOPLASMA GONDII;

BUFFERS; CELL FRACTIONATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXCIPIENTS; GENOMICS; MODELS, BIOLOGICAL; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 84871457453     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0052482     Document Type: Article

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