Overexpression of dsbc and dsbg markedly improves soluble and functional expression of single-chain fv antibodies in escherichia coli

Protein Expression and Purification

Volumn 26, Issue 2, 2002, Pages 218-228

  Zhang, Zhong ^a   Li, Zhi Hua ^a   Wang, Fei ^a   Fang, Min ^a   Yin, Chang Cheng ^a   Zhou, Zhi Yong ^a   Lin, Qing ^a   Huang, Hua Liang ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

Disulfide bond; DsbC; DsbG; Folding; Single chain antibody; Soluble expression

Indexed keywords

ESCHERICHIA COLI;

IMMUNOGLOBULIN FRAGMENT; PRIMER DNA; PROTEIN DISULFIDE ISOMERASE;

ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETICS; IMMUNOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; SOLUBILITY; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOGLOBULIN FRAGMENTS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN DISULFIDE-ISOMERASE; SOLUBILITY;

EID: 0036417347     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00502-8     Document Type: Article

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