Enhanced soluble protein expression using two new fusion tags

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 122-129

  Chatterjee, Deb K ^a   Esposito, Dominic ^a  

^a SAIC FREDERICK INC   (United States)

Author keywords

Fusion proteins; Inclusion bodies; Protein expression; Solubility tag

Indexed keywords

ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; PRIMER DNA; VIRUS PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENE LIBRARY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; RESTRICTION MAPPING;

BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE LIBRARY; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; VIRAL PROTEINS;

ESCHERICHIA COLI;

EID: 32644478232     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.028     Document Type: Article

References (29)

1. R. Rudolph, and H. Lilie In vitro folding of inclusion body proteins FASEB J. 10 1996 49 56
2. D. Christendat, A. Yee, A. Dharamsi, Y. Kluger, M. Gerstein, C.H. Arrowsmith, and A.M. Edwards Structural proteomics: prospects for high throughput sample preparation Prog. Biophys. Mol. Biol. 73 2000 339 345
3. Y. Zhang, D.R. Olsen, K.B. Nguyen, P.S. Olson, E.T. Rhodes, and D. Mascarenhas Expression of eukaryotic proteins in soluble form in Escherichia coli Protein Expr. Purif. 12 1998 159 165
4. H.S. Hwang, and H.S. Chung Preparation of active recombinant cathepsin K expressed in bacteria as inclusion body Protein Expr. Purif. 25 2002 541 546
5. J.G. Thomas, and F. Baneyx Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli Protein Expr. Purif. 11 1997 289 296
6. P.J. Lopez, I. Iost, and M. Dreyfus The use of tRNA as a transcription reporter: the T7 late promoter is extremely efficient in E. coli butits transcripts are poorly expressed Nucleic Acids Res. 22 1994 1186 1193
7. I. Iost, and M. Dreyfus The stability of E. coli lacZ mRNA depends upon the simultaneity of its synthesis and translation EMBO J. 14 1995 3252 3261
8. D.B. Smith, and K.S Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
9. C. di Guan, P. Li, P.D. Riggs, and H. Inouye Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein Gene 15 1988 21 30
10. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.S. Grant, P.F. Schendel, and J.M. McCoy A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Biotechnology 11 1993 187 193
11. G.D. Davis, C. Elisee, D.M. Newman, and R.G. Harrison New fusion protein systems designed to give soluble expression in E. coli Biotechnol. Bioeng. 65 1999 382 388
12. I. Brunoskis, and W.C. Summers The process of infection with bacteriophage T7. VI.A phage gene controlling shutoff of host RNA synthesis Virology 50 1977 322 327
13. L.B. Rothman-Denes, S. Muthukrishnan, R.R. Heselkorn, and F.W. Studier AT7 gene function required for shut-off of host and earlyT7 transcription C.F. Fox W.S. Robinson Virus Research 1973 Academic Press New York 227 239
14. W.T. McAllister, and C.L. Barrett Roles of early genes of bacteriophage T7 in shutoff of host macromolecular synthesis J. Virol. 23 1977 543 553
15. J. Michalewicz, and A.W. Nicholson Molecular cloning and expression of bacteriophage T7 0.7 (protein kinase) gene Virology 186 1992 452 562
16. I. Marchand, A.W. Nicholson, and M. Dreyfus High-level autoenhanced expression of a single-copy gene in Escherichia coli: overproduction of bacteriophage T7 protein kinase directed by T7 late genetic elements Gene 262 2001 231 238
17. I. Marchand, A.W. Nicholson, and M. Dreyfus Bacteriophage T7 protein kinase phosphorylates RNase E and stabilizes mRNAs synthesized by T7 RNA polymerase Mol. Microbiol. 42 2001 767 776
18. L. Hirvas, J. Coleman, P. Koski, and M. Vaara Bacterial 'histone-like protein I' (HLP-I) is an outer membrane constituent? FEBS Lett. 262 1990 123 126
19. P. Koski, L. Hirvas, and M. Vaara Complete sequence of the ompH gene encoding the 16-kDa cationic outer membrane protein of Salmonella typhimurium Gene 88 1990 117 120
20. B.M. Thome, and M. Muller Skp is a periplasmic Escherichia coli protein requiring SecA and SecY for export Mol. Microbiol. 5 1991 2815 2821
21. N. Harms, G. Koningstein, W. Dontje, M. Muller, B. Oudega, J. Luirink, and H. de Cock The early interaction of the outer membrane protein phoE with the periplasmic chaperone Skp occurs at the cytoplasmic membrane J. Biol. Chem. 276 2001 18804 18811
22. R. Levy, R. Weiss, G. Chen, B.L. Iverson, and G. Georgiou Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones Protein Expr. Purif. 23 2001 338 347
23. N. Sheibani Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins Prep. Biochem. Biotechnol. 29 1999 77 90
24. P.A Lund Microbial molecular chaperones Adv. Microb. Physiol. 44 2001 93 140
25. P. Riggs Expression and purification of recombinant proteins by fusion to maltose-binding protein Mol. Biotechnol. 15 2000 51 63
26. R.B. Kapust, and D.S. Waugh Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused Protein Sci. 8 1999 1668 1674
27. G.J. Klarmann, B.M. Eisenhauer, Y. Zhang, K. Sitaraman, D.K. Chatterjee, S.M. Hecht, and S.F. Le Grice Site- and subunit-specific incorporation of unnatural amino acids into HIV-1 reverse transcriptase Protein Expr. Purif. 38 2004 37 44
28. H. Cao, F. Dzineku, and P.J. Blackshear Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases Arch. Biochem. Biophys. 412 2003 106 120
29. S.S. Ashraf, R.E. Benson, E.S. Payne, C.M. Halbleib, and H. Gron A novel multi-affinity tag system to produce high levels of soluble and biotinylated proteins in Escherichia coli Protein Expr. Purif. 33 2004 238 245