The N-domain of Escherichia coli phosphoglycerate kinase is a novel fusion partner to express aggregation-prone heterologous proteins

Biotechnology and Bioengineering

Volumn 109, Issue 2, 2012, Pages 325-335

  Song, Jong Am ^a   Lee, Dae Sung ^a   Park, Jin Seung ^a   Han, Kyung Yeon ^a,b   Lee, Jeewon ^a  

^a Korea University   (South Korea)

^b SAMSUNG Electronics   (South Korea)

Author keywords

Aggregation prone heterologous proteins; E. coli; Fusion partner; N domain of PGK; Phosphoglycerate kinase (PGK)

Indexed keywords

E. COLI; FUSION PARTNER; HETEROLOGOUS PROTEINS; N-DOMAIN OF PGK; PHOSPHOGLYCERATE KINASE;

ARGININE; ENZYMES; ESCHERICHIA COLI; PROTEINS; STABILITY;

BIOSYNTHESIS;

ACTIVATION INDUCED CYTIDINE DEAMINASE; ARGININE; ARGININE DEIMINASE; CUTINASE; FERRITIN; GHRELIN; GLUTAMATE DECARBOXYLASE; GRANULOCYTE COLONY STIMULATING FACTOR; HYBRID PROTEIN; INSULIN; PHOSPHOGLYCERATE KINASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN TARGETING; SOLUBILITY;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; PHOSPHOGLYCERATE KINASE; PROTEIN FOLDING; PROTEINS; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 83555160850     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23320     Document Type: Article

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