Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Schlegel, Susan ^a   Rujas, Edurne ^a   Ytterberg, Anders Jimmy ^b   Zubarev, Roman A ^b   Luirink, Joen ^c   de Gier, Jan Willem ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Escherichia coli; Lemo21(DE3); Periplasm; Protein production; Protein translocation; Recombinant protein; Sec translocon

Indexed keywords

GREEN FLUORESCENT PROTEIN; SIGNAL PEPTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SUPER FOLDER GREEN FLUORESCENT PROTEIN; TRANSLOCON; UNCLASSIFIED DRUG;

ARTICLE; CELL FUNCTION; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; NONHUMAN; PROTEIN AGGREGATION;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; PERIPLASM; PROTEIN DISULFIDE-ISOMERASES; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

ESCHERICHIA COLI;

EID: 84874768785     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-24     Document Type: Article

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