Production of a soluble disulfide bond-linked TCR in the cytoplasm of Escherichia coli trxB gor mutants

Molecular Biotechnology

Volumn 45, Issue 2, 2010, Pages 140-149

  Liddy, Nathaniel ^a   Molloy, Peter E ^a   Bennett, Alan D ^a   Boulter, Jonathan M ^b   Jakobsen, Bent K ^a   Li, Yi ^a  

^a Immunocore Limited   (United Kingdom)

^b CARDIFF UNIVERSITY SCHOOL OF MEDICINE   (United Kingdom)

Author keywords

Cytoplasmic expression; DsbC; Phage display; Skp; T cell receptor; TCR; Thioredoxin reductase; trxB gor Mutant E. coli strains

Indexed keywords

E. COLI; PHAGE DISPLAY; SKP; T-CELL RECEPTORS; THIOREDOXIN REDUCTASE;

CELL CULTURE; CELL MEMBRANES; PROTEINS; SURFACE PLASMON RESONANCE; THROUGHPUT;

ESCHERICHIA COLI;

ANTIGEN; BACTERIAL ENZYME; BACTERIAL PROTEIN; CHAPERONE; DISULFIDE; GLUTATHIONE REDUCTASE; ISOMERASE; OXIDOREDUCTASE; S PHASE KINASE ASSOCIATED PROTEIN; T LYMPHOCYTE RECEPTOR; THIOL DISULFIDE OXIDOREDUCTASE C; THIOREDOXIN REDUCTASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIGEN SPECIFICITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM MUTANT; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GLUTATHIONE REDUCTASE GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; RECEPTOR AFFINITY; SURFACE PLASMON RESONANCE; THIOREDOXIN REDUCTASE GENE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; DISULFIDES; DNA-BINDING PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLUTATHIONE REDUCTASE; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PROTEIN DISULFIDE-ISOMERASES; RECEPTORS, ANTIGEN, T-CELL; SURFACE PLASMON RESONANCE; THIOREDOXIN-DISULFIDE REDUCTASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77954953813     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9250-0     Document Type: Article

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