Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions

Methods in Molecular Biology

Volumn 1033, Issue , 2013, Pages 381-400

  Hjelm, Anna ^a   Schlegel, Susan ^a   Baumgarten, Thomas ^a   Klepsch, Mirjam ^a   Wickström, David ^b   Drew, David ^a   De Gier, Jan Willem ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

E. coli; Fluorescence; FSEC; GFP; Lemo21(DE3); Membrane protein; Overexpression; Purification

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN;

ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

BIOREACTORS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; RECOMBINANT FUSION PROTEINS; TRANSFORMATION, BACTERIAL;

EID: 84884201484     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-487-6_24     Document Type: Article

References (29)

1. Wagner S, Bader ML, Drew D, de Gier JW (2006) Rationalizing membrane protein overexpression. Trends Biotechnol 24:364-371
2. Samuelson JC (2011) Recent developments in difficult protein expression: a guide to E. coli strains, promoters, and relevant host mutations. Methods Mol Biol 705:195-209
3. Wagner S, Klepsch MM, Schlegel S, Appel A, Draheim R, Tarry M, Hogbom M, van Wijk KJ, Slotboom DJ, Persson JO, de Gier JW (2008) Tuning Escherichia coli for membrane protein overexpression. Proc Natl Acad Sci USA 105:14371-14376
4. Schlegel S, Lofblom J, Lee C, Hjelm A, Klepsch M, Strous M, Drew D, Slotboom DJ, de Gier JW (2012) Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3). J Mol Biol 423(4):648-659
5. Studier FW, Moffatt BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113-130
6. Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, Surber MW (2006) Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. Biotechniques 40:355-364
7. Wagner S, Baars L, Ytterberg AJ, Klussmeier A, Wagner CS, Nord O, Nygren PA, van Wijk KJ, de Gier JW (2007) Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics 6:1527-1550
8. Drew DE, von Heijne G, Nordlund P, de Gier JW (2001) Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett 507: 220-224
9. Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, De Gier JW (2005) A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci 14:2011-2017
10. Drew D, Sjostrand D, Nilsson J, Urbig T, Chin CN, de Gier JW, von Heijne G (2002) Rapid topology mapping of Escherichia coli innermembrane proteins by prediction and PhoA/GFP fusion analysis. Proc Natl Acad Sci USA 99:2690-2695
11. Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW (2006) Optimization of membrane protein overexpression and purification using GFP fusions. Nat Methods 3:303-313
12. Newstead S, Kim H, von Heijne G, Iwata S, Drew D (2007) High-throughput fluorescentbased optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 104:13936-13941
13. Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S (2008) GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protoc 3: 784-798
14. Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B (2008) Quality control of overexpressed membrane proteins. Proc Natl Acad Sci USA 105:5722-5727
15. Kawate T, Gouaux E (2006) Fluorescencedetection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14:673-681
16. Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D (2011) Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures. Structure 19:17-25
17. Studier FW (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41:207-234
18. Feilmeier BJ, Iseminger G, Schroeder D, Webber H, Phillips GJ (2000) Green fluorescent protein functions as a reporter for protein localization in Escherichia coli. J Bacteriol 182: 4068-4076
19. Nugent T, Jones DT (2009) Transmembrane protein topology prediction using support vector machines. BMC Bioinformatics 10:159
20. Bernsel A, Viklund H, Hennerdal A, Elofsson A (2009) TOPCONS: consensus prediction of membrane protein topology. Nucleic Acids Res 37:465-468
21. Luirink J, Yu Z, Wagner S, de Gier JW (2012) Biogenesis of inner membrane proteins in Escherichia coli. Biochim Biophys Acta 1817: 965-976
22. Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J (2010) Bridging the gap: a GFP-based strategy for overexpression and purifi-cation of membrane proteins with intra and extracellular C-termini. Protein Sci 19:868-880
23. Low C, Jegerschold C, Kovermann M, Moberg P, Nordlund P (2012) Optimisation of overexpression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1. PLoS One 7:e38244
24. http://www.piercenet.com/browse.cfm?fldID= 02020101
25. Strop P, Brunger AT (2005) Refractive indexbased determination of detergent concentration and its application to the study of membrane proteins. Protein Sci 14:2207-2211
26. Gonzales EB, Kawate T, Gouaux E (2009) Pore architecture and ion sites in acid-sensing ion channels and P2X receptors. Nature 460:599-604
27. Kawate T, Michel PO, Birdsong WT, Gouaux E (2009) Crystal structure of the ATP-gated P2X(4) ion channel in the closed state. Nature 460:592-598
28. Guan L, Mirza O, Verner G, Iwata S, Kaback HR (2007) Structural determination of wildtype lactose permease. Proc Natl Acad Sci USA 104:15294-15298
29. Drew D, Klepsch MM, Newstead S, Flaig R, De Gier JW, Iwata S, Beis K (2008) The structure of the efflux pump AcrB in complex with bile acid. Mol Membr Biol 25:677-682