Use of quantitative PCR detection methods to study biocontrol agents and phytopathogenic fungi and oomycetes in environmental samples

Journal of Phytopathology

Volumn 162, Issue 1, 2014, Pages 1-13

  Sanzani, Simona M ^a   Li Destri Nicosia, Maria G ^b   Faedda, Roberto ^c   Cacciola, Santa O ^c   Schena, Leonardo ^b  

^a UNIVERSITY OF BARI   (Italy)

^b UNIVERSITY MEDITERRANEA OF REGGIO CALABRIA   (Italy)

^c UNIVERSITY OF CATANIA   (Italy)

Author keywords

Biocontrol agents; Detection; Mycotoxigenic fungi; Phytopathogenic fungi; Phytopathogenic oomycetes; QPCR; Quantitative analyses

Indexed keywords

BIOCONTROL AGENT; BIOLOGY; BREEDING; CRITICAL ANALYSIS; DETECTION METHOD; DNA; EPIDEMIOLOGY; FUNGUS; PATHOLOGY; PLANT; POLYMERASE CHAIN REACTION;

FUNGI; OOMYCETES;

EID: 84889670117     PISSN: 09311785     EISSN: 14390434     Source Type: Journal    
DOI: 10.1111/jph.12147     Document Type: Review

References (108)

1. Atkins SD, Clark IM, Sosnowska D, Hirsch PR, Kerry BR. (2003) Detection and quantification of Plectosphaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media, and baiting. Appl Environ Microbiol 69:4788-4793.
2. Atoui A, El Khoury A, Kallassy M, Lebrihi A. (2012) Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize. Int J Food Microbiol 154:259-265.
3. Bilodeau GJ. (2011) Quantitative polymerase chain reaction for the detection of organisms in soil. CAB Rev: Perspect Agr, Vet Sci, Nutr Natl Res 6:14.
4. Bilodeau GJ, Koike ST, Uribe P, Martin FN. (2012) Development of an assay for rapid detection and quantification of Verticillium dahliae in soil. Phytopathology 102:331-343.
5. Bithell SL, McKay A, Butler RC, Herdina Ophel-Keller K, Hartley D, Cromey MG. (2012) Predicting Take-All severity in second-year wheat using soil DNA concentrations of Gaeumannomyces graminis var. tritici determined with qPCR. Plant Dis 96:443-451.
6. Blanco-Meneses M, Ristaino JB. (2011) Detection and quantification of Peronospora tabacina using a real-time polymerase chain reaction assay. Plant Dis 95:673-682.
7. Bodles WJA, Fossdal CG, Woodward S. (2006) Multiplex real-time PCR detection of pathogen colonization in the bark and wood of Picea sitchensis clones differing in resistance to Heterobasidion annosum. Tree Physiol 26:775-782.
8. Boedo C, Le Clerc V, Briard M, Simoneau P, Chevalier M, Georgeault S, Poupard P. (2008) Impact of carrot resistance on development of the Alternaria leaf blight pathogen (Alternaria dauci). Eur J Plant Pathol 121:55-66.
9. Boutigny AL, Beukes I, Small I, Zühlke S, Spiteller M, Van Rensburg BJ, Flett B, Viljoen A. (2012) Quantitative detection of Fusarium pathogens and their mycotoxins in South African maize. Plant Pathol 61:522-531.
10. Boutigny AL, Guinet C, Vialle A, Hamelin R, Frey P, Ioos R. (2013) A sensitive Real time PCR assay for the detection of the two Melampsora medusae formae speciales on infected poplar leaves. Eur J Plant Pathol 136:433-441.
11. Carisse O, Tremblay DM, Lévesque CA, Gindro K, Ward P, Houde A. (2009) Development of a TaqMan real-time PCR assay for quantification of airborne conidia of Botrytis squamosa and management of Botrytis leaf blight of onion. Phytopathology 99:273-1280.
12. Carnegie AJ, Lidbetter JR, Walker J, Horwood MA, Tesoriero L, Glen M, Priest MJ. (2010) Uredo rangelii, a taxon in the guava rust complex, newly recorded on Myrtaceae in Australia. Australas Plant Pathol 39:463-466.
13. Chandelier A, Planchon V, Oger R. (2010) Determination of cycle cut off in real-time PCR for the detection of regulated plant pathogens. Bull OEPP 40:52-58.
14. Chimento A, Cacciola SO, Garbelotto M. (2012) Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum. Forest Pathol 42:14-21.
15. Cooke DEL, Schena L, Cacciola SO. (2007) Tools to detect, identify and monitor Phytophthora species in natural ecosystems. J Plant Pathol 89:13-28.
16. Cordier C, Edel-Hermann V, Martin-Laurent F, Blal B, Steinberg C, Alabouvette C. (2007) SCAR-based real time PCR to identify a biocontrol strain (T1) of Trichoderma atroviride and study its population dynamics in soils. J Microbiol Methods 68:60-68.
17. Covarelli L, Beccari G, Steed A, Nicholson P. (2012) Colonization of soft wheat following infection of the stem base by Fusarium culmorum and translocation of deoxynivalenol to the head. Plant Pathol 61:1121-1129.
18. Cullen DW, Lees AK, Toth IK, Duncan JM. (2002) Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR. Plant Pathol 51:281-292.
19. De Coninck BMA, Amand O, Delauré SL, Lucas S, Hias N, Weyens G, Mathys J, de Bruyne E, Cammue BPA. (2012) The use of digital image analysis and real-time PCR fine-tunes bioassays for quantification of Cercospora leaf spot disease in sugar beet breeding. Plant Pathol 61:76-84.
20. Dean R, Van Kan JAL, Pretorius ZA et al. (2012) The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13:410-430.
21. Debode J, Van Hemelrijck W, Baeyen S, Creemers P, Heungens K, Maes M. (2009) Quantitative detection and monitoring of Colletotrichum acutatum in strawberry leaves using real-time PCR. Plant Pathol 58:504-514.
22. ® technologies for specific quantitative detection of Phoma tracheiphila in infected Citrus. Eur J Plant Pathol 120:339-351.
23. Diguta CF, Rousseaux S, Weidmann S, Bretin N, Vincent B, Guilloux-Benatier M, Herve A. (2010) Development of a qPCR assay for specific quantification of Botrytis cinerea on grapes. FEMS Microbiol Lett 313:81-87.
24. Divon HH, Razzaghian J, Udnes-Aamot H, Klemsdal SS. (2012) Fusarium langsethiae (Torp and Nirenberg), investigation of alternative infection routes in oats. Eur J Plant Pathol 132:147-161.
25. Duvivier M, Dedeurwaerder G, Marchal G, Renard ME, Van Hese V, Moreau JM, Legreve A. (2010) Distribution of airborne Mycosphaerella graminicola inoculum at the field scale. Commun Agric Appl Biol Sci 75:635-639.
26. Erwin DC, Ribeiro OK. (1996) Phytophthora Diseases Worldwide. St. Paul, MN, APS Press.
27. Faedda R, Cacciola SO, Szigethy A, Bakonyi J, Man in't Veld W, Martini P, Pane A, Schena L, Magnano di San Lio G. (2013) Phytophthora × pelgrandis causes root and collar rot of Lavandula stoechas in Italy. Plant Dis. doi: 10.1094/PDIS-11-12-1035-RE (in press).
28. Filion M, St-Arnaud M, Jabaji-Hare SH. (2003) Quantification of Fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. Phytopathology 93:229-235.
29. Fountaine JM, Shaw MW, Ward E, Fraaije BA. (2010) The role of seeds and airborne inoculum in the initiation of leaf blotch (Rhynchosporium secalis) epidemics in winter barley. Plant Pathol 59:330-337.
30. Garbelotto M, Smith T, Schweigkofler W. (2008) Variation of spore dispersal of Fusarium circinatum, the causal agent of pine pitch canker, over a 12-month-period at two locations in Northern California. Phytopathology 98:137-143.
31. Gayoso C, Martínez de Ilárduya O, Pomar F, Merino de Cáceres F. (2007) Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars. Eur J Plant Pathol 118:199-209.
32. Gil-Serna J, González-Salgado A, González-Jaén MT, Vázquez C, Patiño B. (2009) ITS-based detection and quantification of Aspergillus ochraceus and Aspergillus westerdijkiae in grapes and green coffee beans by real-time quantitative PCR. Int J Food Microbiol 131:162-167.
33. Gullino ML, Migheli Q, Mezzalama M. (1995) Risk analysis in the release of biological control agents. Plant Dis 79:1193-1201.
34. Guo Y, Li W, Sun H, Wang N, Yu H, Chen H. (2012) Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. J Gen Plant Pathol 78:247-254.
35. Hamada MS, Yin YN, Ma ZH. (2012) Rapid detection of Fusarium graminearum complex in wheat seeds using species-specific PCR primer designed based on a microsatellite region. Cereal Res Commun 40:85-94.
36. Hietala AM, Eikenes M, Kvaalen H, Solheim H, Fossdal CG. (2003) Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in norway spruce clones that differ in disease resistance. Appl Environ Microbiol 69:4413-4420.
37. Hong CX, Moorman GW. (2005) Plant pathogens in irrigation water: challenges and opportunities. Crit Rev Plant Sci 24:189-208.
38. Huang C, Sun Z, Yan J, Luo Y, Wang H, Ma Z. (2011) Rapid and precise detection of latent infections of wheat stripe rust in wheat leaves using loop-mediated isothermal amplification. J Phytopathol 159:582-584.
39. Ioos R, Fourrier C, Wilson V, Webb K, Schereffer J-L, Tourvieille de Labrouh D. (2012) An optimized duplex Real-Time PCR tool for sensitive detection of the quarantine oomycete Plasmopara halstedii in sunflower seeds. Phytopathology 102:908-917.
40. Ippolito A, Schena L, Nigro F, Soleti Logorio V, Yaseen T. (2004) Real-time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil. Eur J Plant Pathol 110:833-843.
41. Jackson SL, Bayliss KL. (2011) Spore traps need improvement to fulfil plant biosecurity requirements. Plant Pathol 60:801-810.
42. Jiménez-Fernández D, Montes-Borrego M, Navas-Cortés JA, Jiménez-Díaz RM, Landa BB. (2010) Identification and quantification of Fusarium oxysporum in planta and soil by means of an improved specific and quantitative PCR assay. Appl Soil Ecol 46:372-382.
43. Kernaghan G, Reeleder RD, Hoke SMT. (2007) Quantification of Cylindrocarpon destructans f. sp. panacis in soils by real-time PCR. Plant Pathol 56:508-516.
44. Köhl J, Vlaswinkel M, Groenenboom-de Haas BH, Kastelein P, van Hoof RA, van der Wolf JM, Krijger M. (2011) Survival of pathogens of Brussels sprouts (Brassica oleracea Gemmifera Group) in crop residues. Plant Pathol 60:661-670.
45. Kulik T, Jestoi M, Okorski A. (2011) Development of TaqMan assays for the quantitative detection of Fusarium avenaceum/Fusarium tricinctum and Fusarium poae esyn1 genotypes from cereal grain. FEMS Microbiol Lett 314:49-56.
46. Lane CR, Beales PA, Hughes JD. (2012) Fungal Plant Pathogens. Wallingford, UK, CABI.
47. Lees AK, Sullivan L, Lynott JS, Cullen DW. (2012) Development of a quantitative real-time PCR assay for Phytophthora infestans and its applicability to leaf, tuber and soil samples. Plant Pathol 6:867-876.
48. Li Y, Minerdi D, Garibaldi A, Gullino ML. (2009) Molecular detection of Phytophthora cryptogea on Calendula officinalis and Gerbera jamesonii artificially inoculated with zoospores. J Phytopathol 157:438-445.
49. Lievens B, Brouwer M, Vanachter ACRC, Cammue BPA, Thomma BPHJ. (2006) Real-time PCR for detection and quantification of fungal and oomycete tomato pathogens in plant and soil samples. Plant Sci 171:155-165.
50. Lin Y-H, Su C-C, Chao C-P, Chen C-Y, Chang C-J, Huang J-W, Chang P-FL. (2013) A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4. Eur J Plant Pathol 135:395-405.
51. Llorente B, Bravo-Almonacid F, Cvitanich C, Orlowska E, Torres HN, Flawia MM, Alonso GD. (2010) A quantitative real-time PCR method for in planta monitoring of Phytophthora infestans growth. Lett Appl Microbiol 51:603-610.
52. López-Mondéjar R, Antón A, Raidl S, Ros M, Pascual JA. (2010) Quantification of the biocontrol agent Trichoderma harzianum with real-time TaqMan PCR and its potential extrapolation to the hyphal biomass. Bioresour Technol 101:2888-2891.
53. López-Mondéjar R, Beaulieu R, Ros M, Pascual JA. (2012) SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions. Crop Prot 33:1-6.
54. Luo Y, Ma Z, Michailides TJ. (2007a) Quantification of allele E198A in beta-tubulin conferring benzimidazole resistance in Monilinia fructicola using real-time PCR. Pest Manag Sci 63:1178-1184.
55. Luo Y, Ma Z, Reyes HC, Morgan DP, Michailides TJ. (2007b) Using real-time PCR to survey frequency of azoxystrobin-resistant allele G143A in Alternaria populations from almond and pistachio orchards in California. Pestic Biochem Physiol 88:328-336.
56. Luo Y, Gao W, Doster M, Michailides TJ. (2009) Quantification of conidial density of Aspergillus flavus and A. parasiticus in soil from almond orchards using real-time PCR. J Appl Microbiol 106:1649-1660.
57. Maciá-Vicente JG, Rosso LC, Ciancio A, Jansson HB, Lopez-Llorca LV. (2009) Colonisation of barley roots by endophytic Fusarium equiseti and Pochonia chlamydosporia: effects on plant growth and disease. Ann Appl Biol 155:391-401.
58. Martín MT, Cobos R, Martín L, López-Enríquez L. (2012) Real-Time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum. Appl Environ Microbiol 78:3985-3991.
59. Mayer Z, Bagnara A, Paul Färber, Geisen R. (2003) Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. Int J Food Microbiol 82:143-151.
60. Mercado-Blanco J, Collado-Romero M, Parrilla-Araujo S, Rodríguez-Jurado D, Jiménez-Díaz RM. (2003) Quantitative monitoring of colonization of olive genotypes by Verticillium dahliae pathotypes with real-time polymerase chain reaction. Physiol Mol Plant Pathol 63:91-105.
61. Michalecka M, Malinowski T, Broniarek-Niemiec A, Bielenin A. (2011) Real-time PCR assay with SNP-specific primers for the detection of a g143a mutation level in Venturia inaequalis field populations. J Phytopathol 159:569-578.
62. Minerdi D, Moretti M, Li Y, Gaggero L, Garibaldi A, Gullino ML. (2008) Conventional PCR and real time quantitative PCR detection of Phytophthora cryptogea on Gerbera jamesonii. Eur J Plant Pathol 122:227-237.
63. Montes-Borrego M, Muñoz-Ledesma FJ, Jiménez-Díaz RM, Landa BB. (2011) Real-time PCR quantification of Peronospora arborescens, the opium poppy downy mildew pathogen, in seed stocks and symptomless infected plants. Plant Dis 95:143-152.
64. Mumford R, Boonham N, Tomlinson J, Barker I. (2006) Advances in molecular phytodiagnostics new solutions for old problems. Eur J Plant Pathol 116:1-19.
65. Nirenberg HI, Gerlach WF, Grafenhan T. (2009) Phytophthora pelgrandis, a new natural hybrid pathogenic to Pelargonium grandiflorum hort. Mycologia 10:220-231.
66. O'Brien PA, Nari W, StJ Hardy GE. (2009) Detecting Phytophthora. Crit Rev Microbiol 35:169-181.
67. Okubara PA, Schroeder KL, Paulitz TC. (2005) Real-time polymerase chain reaction: applications to studies on soilborne pathogens. Can J Plant Pathol 27:300-313.
68. Ophel-Keller K, McKay A, Hartley D, Curran H, Curran J. (2008) Development of a routine DNA-based testing service for soilborne diseases in Australia. Australas Plant Pathol 37:243-253.
69. Pavón CF, Babadoost M, Lambert KN. (2008) Quantification of Phytophthora capsici oospores in soil by sieving-centrifugation and real-time polymerase chain reaction. Plant Dis 92:143-149.
70. Pilotti M, Lumia V, Di Lernia G, Brunetti A. (2012) Development of Real-Time PCR for in wood-detection of Ceratocystis platani, the agent of canker stain of Platanus spp. Eur J Plant Pathol 134:61-79.
71. Qi M, Yang Y. (2002) Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphoimaging analyses. Phytopathology 92:870-876.
72. Richards TA, Dacks JB, Jenkinson JM, Thornton CR, Talbot NJ. (2006) Evolution of filamentous plant pathogens: gene exchange across eukaryotic kingdoms. Curr Biol 16:1857-1864.
73. Rodríguez A, Rodríguez M, Andrade MJ, Córdoba JJ. (2012) Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods. Int J Food Microbiol 155:10-18.
74. Rodríguez-Molina MC, Tello-Marquina JC, Torres-Vila LM, Bielza-Lino P. (2000) Micro-scale systematic sampling of soil: heterogeneity in populations of Fusarium oxysporum, F. solani, F. roseum and F. moniliforme. J Phytopathol 148:609-614.
75. Rogers SL, Atkins SD, West JS. (2009) Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCR. Plant Pathol 58:324-331.
76. Ruano-Rosa D, Schena L, Ippolito A, López-Herrera CJ. (2007) Comparison of conventional and molecular methods for the detection of Rosellinia necatrix in avocado orchards in southern Spain. Plant Pathol 56:251-256.
77. Sanzani SM, Schena L, Nigro F, De Girolamo A, Ippolito A. (2009) Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis. Eur J Plant Pathol 125:223-233.
78. Sanzani SM, Schena L, De Cicco V, Ippolito A. (2012a) Early detection of Botrytis cinerea latent infections as a tool to improve postharvest quality of table grapes. Postharvest Biol Technol 68:64-71.
79. Sanzani SM, Reverberi M, Punelli M, Ippolito A, Fanelli C. (2012b) Study on the role of patulin on pathogenicity and virulence of Penicillium expansum. Int J Food Microbiol 153:323-331.
80. Sanzani SM, Montemurro C, Di Rienzo V, Solfrizzo M, Ippolito A. (2013) Genetic structure and natural variation associated with host of origin in Penicillium expansum strains causing blue mould. Int J Food Microbiol 165:111-120.
81. Schena L, Ippolito A. (2003) Rapid and sensitive detection of Rosellinia necatrix in roots and soils by real time scorpion-PCR. J Plant Pathol 85:15-25.
82. Schena L, Finetti Sialer M, Gallitelli D. (2002) Molecular detection of the strain L47 of Aureobasidium pullulans, a biocontrol agent of postharvest diseases. Plant Dis 86:54-60.
83. Schena L, Nigro F, Ippolito A, Gallitelli D. (2004) Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. Eur J Plant Pathol 110:893-908.
84. Schena L, Hughes KJD, Cooke DEL. (2006) Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real-time PCR. Mol Plant Pathol 7:365-379.
85. Schena L, Li Destri Nicosia MG, Sanzani SM, Faedda R, Ippolito A, Cacciola SO. (2013) Development of quantitative PCR detection methods for phytopathogenic fungi and oomycetes. J Plant Pathol 95:7-24.
86. Schmittgen TD. (2001) Real-time quantitative PCR. Methods 25:383-385.
87. ®Green I for continuous fluorescence monitoring. Int J Food Microbiol 71:53-61.
88. Scibetta S, Schena L, Cimento A, Cacciola SO, Cooke DEL. (2012) A molecular method to assess Phytophthora diversity in environmental samples. J Microbiol Methods 88:356-368.
89. Shishido M, Kubota I, Ohashi T, Usami T. (2013) Comparison of PCR assays for detection and quantification of Phomopsis sclerotioides in plant and soil. J Gen Plant Pathol 79:18-27.
90. Spies CFJ, Mazzola M, McLeod A. (2011) Characterisation and detection of Pythium and Phytophthora species associated with grapevines in South Africa. Eur J Plant Pathol 131:103-119.
91. Stewart-Wade SM. (2011) Plant pathogens in recycled irrigation water in commercial plant nurseries and greenhouses: their detection and management. Irrig Sci 29:267-297.
92. Tao G, Hyde KD, Cai L. (2013) Species-specific real-time PCR detection of Colletotrichum kahawae. J Appl Microbiol 114:828-835.
93. Thomidis T, Michailides TJ. (2010) Development and implementation of cost-effective strategies to manage brown rot of peach trees in Imathia, Greece. Eur J Plant Pathol 126:575-582.
94. Tsao PH. (1970) Selective media for isolation of pathogenic fungi. Annu Rev Phytopathol 8:157-186.
95. Uyttendaele M, Van Boxstael S, Debevere J. (1999) PCR assay for detection of the E. Coli O157:H7 eae-gene and effect of the sample preparation method on PCR detection of heat-killed E. coli O157:H7 in ground beef. Int J Food Microbiol 52:85-95.
96. Vallance J, Le Floch G, Déniel F, Barbier G, Lévesque CA, Rey P. (2009) Influence of Pythium oligandrum biocontrol on fungal and Oomycete population dynamics in the rhizosphere. Appl Environ Microbiol 75:4790-4800.
97. Van Gent-Pelzer MPE, Krijger M, Bonants PJM. (2010) Improved real-time PCR assay for detection of the quarantine potato pathogen, Synchytrium endobioticum, in zonal centrifuge extracts from soil and in plants. Eur J Plant Pathol 126:129-133.
98. Vandemark GJ, Barker BM. (2003) Quantifying Phytophthora medicaginis in susceptible and resistant alfalfa with a real-time fluorescent PCR assay. J Phytopathol 151:577-583.
99. Venette RC, Kriticos DJ, Magarey RD et al. (2010) Pest risk maps for invasive alien species: a roadmap for improvement. Bioscience 60:349-362.
100. Vincelli P, Tisserat N. (2008) Nucleic acid based pathogen detection in applied plant pathology. Plant Dis 92:660-669.
101. Wilson IG, Cooper JE, Gilmour A. (1994) Some factors inhibiting amplification of the Staphylococcus aureus enterotoxin C1 (sec1) by PCR. Int J Food Microbiol 22:55-62.
102. Wolffs P, Norling B, Rådström P. (2005) Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells. J Microbiol Methods 60:315-323.
103. Woodhall JW, Adams IP, Peters JC, Harper G, Boonham N. (2013) A new quantitative real-time PCR assay for Rhizoctonia solani AG3-PT and the detection of AGs of Rhizoctonia solani associated with potato in soil and tuber samples in Great Britain. Eur J Plant Pathol 136:273-280.
104. Yamamoto N, Kimura M, Matsuki H, Yanagisawa Y. (2010) Optimization of a real-time PCR assay to quantitate airborne fungi collected on a gelatin filter. J Biosci Bioeng 109:83-88.
105. Yan J, Luo Y, Chen T, Huang C, Ma Z. (2012) Field distribution of wheat stripe rust latent infection using real-time PCR. Plant Dis 96:544-551.
106. Zeng X, Luo Y, Zheng Y, Duan X, Zhou Y. (2010) Detection of latent infection of wheat leaves caused by Blumeria graminis f.sp. tritici using nested PCR. J Phytopathol 158:227-235.
107. Zhang ZG, Li YQ, Fan H, Wang YC, Zheng XB. (2006) Molecular detection of Phytophthora capsici in infected plant tissues, soil and water. Plant Pathol 55:770-775.
108. Zhao S, Clarke BB, Shen Q, Zhang L, Zhang N. (2012) Development and application of a TaqMan real-time PCR assay for rapid detection of Magnaporthe poae. Mycologia 104:1250-1259.