Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods

International Journal of Food Microbiology

Volumn 82, Issue 2, 2003, Pages 143-151

  Mayer, Zsuzsanna ^a   Bagnara, Angelo ^b   Färber, Paul ^b   Geisen, Rolf ^b  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

^b FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

Author keywords

Aflatoxin; Aspergillus flavus; Maize; nor 1; Paprika; Pepper; Quantification; Real time PCR

Indexed keywords

AFLATOXIN; FLUORESCENT DYE;

ARTICLE; ASPERGILLUS FLAVUS; COLONY FORMING UNIT; CONTROLLED STUDY; CORRELATION ANALYSIS; DEVICE; FOOD; FOOD CONTAMINATION; FUNGAL CONTAMINATION; FUNGAL DETECTION; FUNGAL GENE; FUNGAL STRAIN; GENE IDENTIFICATION; MAIZE; NONHUMAN; NOR 1 GENE; PEPPER; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

AFLATOXINS; ALCOHOL OXIDOREDUCTASES; ASPERGILLUS FLAVUS; BASE SEQUENCE; CAPSICUM; COLONY COUNT, MICROBIAL; DNA, FUNGAL; FUNGAL PROTEINS; GENES, FUNGAL; POLYMERASE CHAIN REACTION; TAQ POLYMERASE; ZEA MAYS;

ASPERGILLUS; ASPERGILLUS FLAVUS; ZEA MAYS;

EID: 0037466440     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(02)00250-7     Document Type: Article

References (26)

1. Barbesgaard P., Heldt-Hansen H.P., Diderichsen B. On the safety of Aspergillus oryzae: a review. Appl. Microbiol. Biotechnol. 36:1992;569-572.
2. Bartine H., Tantaoui-Elaraki A. Growth and toxigenesis of Aspergillus flavus isolates on selected spices. J. Environ. Pathol. Toxicol. Oncol. 16:1997;61-65.
3. Böhm J., Hahn A., Schubert R., Bahnweg G., Adler N., Nechwatal J., Oehlmann R., Oβwald W. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophtora infestans and Phytophtora citricola in their respective host plants. J. Phytopathol. 147:1999;409-416.
4. Bowman J.C., Abruzzo G.K., Anderson J.W., Flattery A.M., Gill C.J., Pikounis V.B., Schmatz D.M., Liberator P.A., Douglas C.M. Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate. Antimicrob. Agents Chemother. 45:2001;3474-3481.
5. Burnett J.H. Mycogenetics. 1975;Wiley, London.
6. De Ruiter G.A., Notermanns S.H.W., Rombouts F.M. New methods in food mycology. Trends Food Sci. Technol. 4:1993;91-97.
7. Färber P., Geisen R., Holzapfel W.H. Detection of aflatoxinogenic fungi in figs by a PCR reaction. Int. J. Food Microbiol. 36:1997;215-220.
8. Geisen R. A multiplex PCR reaction for the detection of aflatoxin and sterigmatocystin producing fungi. Syst. Appl. Microbiol. 19:1996;388-392.
9. Gourama H., Bullermann L.B. Detection of molds in foods and feeds: potential rapid and selective methods. J. Food Protect. 58:1995;1389-1394.
10. Gow N.A.R., Gadd G.M. The Growing Fungus. 1995;Chapman & Hall, London.
11. Haugland R.A., Vesper S.J., Wymer L.J. Quantitative measurement of Stachybotrys chartrarum conidia using real time detection of PCR products with the TaqMan™ fluorogenic probe system. Mol. Cell. Probes. 13:1999;329-340.
12. Ito Y., Peterson S.W., Wicklow D.T., Goto T. Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi. Mycol. Res. 105:2001;233-239.
13. Jelinek C.F., Pohland A.E., Wood G.E. Worldwide occurrence of mycotoxins in foods and feeds - an update. J. Assoc. Off. Anal. Chem. 72:1989;223-230.
14. Kaminskyj S.G., Hamer J.E. Hyp loci control cell pattern formation in the vegetative mycelium of Aspergillus nidulans. Genetics. 148:1998;669-680.
15. Klich M., Yu J., Chang P.K., Mullaney E.J., Bhatnagar D., Cleveland T. Hybridization of genes involved in aflatoxin biosynthesis to DNA of aflatoxinogenic and non-aflatoxinogenic aspergilli. Appl. Microbiol. Biotechnol. 44:1995;439-443.
16. Pittet A. Natural occurrence of mycotoxins in foods and feeds - an updated review. Rev. Vet. Med. 149:1988;479-492.
17. Rossen L., Norskov P., Holmstrom K., Rasmussen O.F. Inhibition of PCR by components of food samples microbial diagnostic assays and DNA-extraction solutions. Int. J. Food Microbiol. 17:1992;37-45.
18. Schnerr H., Niessen M.L., Vogel R.F. Real Time detection of the tri5 gene in Fusarium species by LightCycler™-PCR using SYBR Green I for continuous fluorescence monitoring. Int. J. Food Microbiol. 71:2001;53-61.
19. Schnürer J., Olsson J., Börjesson T. Fungal volatiles as indicators of food and feed spoilage. Fungal Genet. Biol. 27:1999;209-217.
20. Seitz L.M., Mohr H.E., Burroughs R., Sauer D.B. Ergosterol as an indicator of fungal invasion in grains. Cereal Chem. 54:1977;1207-1217.
21. Shapira R., Paster N., Eyal O., Menasherov M., Mett A., Salomon R. Detection of aflatoxinogenic molds in grains by PCR. Appl. Environ. Microbiol. 62:1996;3270-3273.
22. Spangenberg D.S., Ingham S.C. Comparison of methods for enumeration of yeasts and molds in shredded low-moisture, part-skim Mozzarella cheese. J. Food Protect. 63:2000;529-533.
23. Sweeney M.J., Pamies P., Dobson A.D.W. The use of reverse transcription polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439. Int. J. Food Microbiol. 56:2000;97-103.
24. Trail F., Chang P.K., Cary J.W., Linz J. Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl. Environ. Microbiol. 60:1994;4078-4085.
25. Woloshuk C.P., Prieto R. Genetic organization and function of the aflatoxin B1 biosynthetic genes. FEMS Microbiol. Lett. 160:1998;169-176.
26. Yelton M.M., Hamer J.E., Timberlake W.E. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc. Natl. Acad. Sci. U. S. A. 81:1984;1470-1474.