Development and application of a TaqMan real-time PCR assay for rapid detection of Magnaporthe poae

Mycologia

Volumn 104, Issue 5, 2012, Pages 1250-1259

  Zhao, Shuang ^a,b   Clarke, Bruce B ^b   Shen, Qirong ^a   Zhang, Lisa ^c   Zhang, Ning ^b  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b RUTGERS UNIVERSITY   (United States)

^c East Brunswick High School   (United States)

Author keywords

Diagnostic assay; Fungicide; Molecular detection; Plant pathogen; Poaceae; Qpcr; Turf; Turfgrass

Indexed keywords

FUNGAL DNA;

DETECTION METHOD; DISEASE SEVERITY; FUNGICIDE; FUNGUS; GRASS; MOLECULAR ANALYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; REAL TIME; ROOT SYSTEM; VISUAL ANALYSIS;

ARTICLE; GENETICS; ISOLATION AND PURIFICATION; MAGNAPORTHE; METHODOLOGY; MICROBIOLOGY; PLANT DISEASE; PLANT ROOT; POA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

DNA, FUNGAL; MAGNAPORTHE; PLANT DISEASES; PLANT ROOTS; POA; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

NORTH AMERICA;

FESTUCA; MAGNAPORTHE POAE; POA; POA PRATENSIS; POACEAE;

EID: 84867176281     PISSN: 00275514     EISSN: 15572536     Source Type: Journal    
DOI: 10.3852/11-365     Document Type: Article

References (42)

1. Augustin C, Ulrich K, Ward E, Werner A. 1999. RAPD-based inter- and intravarietal classfication of fungi of the Gaeumannomyces-Phialophora complex. J Phytopathol 147:109-117.
2. Bryan GT, Daniels MJ, Osbourn AE. 1995. Comparison of fungi within the Gaeumannomyces-Phialophora complex by analysis of ribosomal DNA sequences. Appl Environ Microbiol 61:681-689.
3. Bunting TE, Plumley KA, Clarke BB, Hillman BI. 1996. Identification of Maganporthe poae by PCR and examiniation of its relationship to other fungi by analysis of their nuclear rDNA ITS-1 region. Mol Plant Pathol 86:399-404.
4. Clarke BB, Majumdar P, Fitzgerald D, Flynn T, Peacos M, Pitonak A, Jepsen L, Dickson WK, Clark J, Lawson TJ, Scholz A. 2009. Influence of fungicides and biorational products on the development of summer patch in Kentucky bluegrass. In: Gould AB, ed. Rutgers Turfgrass Proceedings. New Brunswick, New Jersey: Center for Turfgrass Science and the New Jersey Turfgrass Association. Vol. 40. p 325-329.
5. Dötsch J, Repp R, Rascher W, Christiansen H. 2001. Diagnostic and scientific applications of TaqMan realtime PCR in neuroblastomas. Expert Rev Mol Diagn 1: 233-238
6. Fouly HM, Wilkinson HT. 2000. Detection of Gaeumannomyces graminis varieties using polymerase chain reaction with variety-specific primers. Plant Dis 84:947-951
7. Gardes M, Bruns TD. 1993. ITS primers with enhanced specificity for Basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2: 113-118
8. Gibson UE, Heid CA, Williams PM. 1996. A novel method for real-time quantitative RT-PCR. Genome Res 6:995- 1001
9. Gruber F, Falkner FG, Dorner F, Hammerle T. 2001. Quantitation of viral DNA by real-time PCR applying duplex amplification, internal standardization, and two-color fluorescence detection. Appl Environ Microbiol 67:2837-2839
10. Harmon PF, Dunkle LD, Latin R. 2003. A rapid PCR-based method for the detection of Magnaporthe oryzae from infected perennial ryegrass. Plant Dis 87:1072-1076
11. Henson JM. 1992. DNA hybridization and polymerase chain reaction (PCR) tests for identification of Gaeumannomyces, Phialophora and Magnaporthe isolates. Mycol Res 96:629-636
12. Henson JM. French R. 1993. The polymerase chain reaction and plant disease diagnosis. Annu Rev Phytopathol 31:81- 109
13. Higuchi R, Fockler C, Dollinger G, Watson R. 1993. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Nat Biotechnol 11:1026-1030
14. Hogg AC, Johnston RH, Dyer AT. 2007. Applying real-time quantitative PCR to Fusarium crown rot of wheat. Plant Dis 91:1021-1028
15. Juhnke ME, Mathre DE, Sands DC. 1984. A selective medium for Gaeumannomyces graminis var. tritici. Plant Dis 68:233-236.
16. Kackley KE, Grybauskas AP, Dernoeden PH. 1990. Growth of Magnaporthe poae and Gaeumannomyces incrustans as affected by temperature-osmotic potential interactions. Phytopathology 80:646-650
17. Kackley KE, Grybauskas AP, Dernoeden PH. Hill RL. 1990. The role of drought stress in the development of summer patch in fieldinoculated Kentucky bluegrass. Phytopathology 80: 655-658
18. Kemp ML, Clarke BB, Funk CR. 1990. The susceptibility of fine fescues to isolates of Magnaporthe poae and Gaeumannomyces incrustans. Phytopathology 80:978.
19. Klein D. 2002. Quantification using real-time PCR technology: applications and limitations. Trends Mol Med 8: 257-260.
20. Landschoot PJ. 1992. Magnaporthe. In: Singleton LL, Mihail JD, Rush CM, eds. Methods for research on soilborne phytopathogenic fungi. St Paul, Minnesota: American Phytopathological Society Press. p 67-70.
21. Landschoot PJ. 1993. Taxonomy and biology of ectotrophic rootinfecting fungi associated with patch diseases of turfgrasses. In: Clarke BB, Gould AB, eds. Turfgrass patch diseases caused by ectotrophic root-infecting fungi. St Paul, Minnesota: American Phytopathological Society Press. p 41-71.
22. Landschoot PJ. Clarke BB, Gould AB. 1993. Ecology and epidemiology of ectotrophic root-infecting fungi associated with patch diseases of Turfgrass. In: Clarke BB, Gould AB, eds. Turfgrass patch diseases caused by ectotrophic root-infecting fungi. St Paul, Minnesota: American Phytopathological Society Press. p 73-105.
23. Landschoot PJ. Jackson N. 1990. Pathogenicity of some ectotrophic fungi with Phialophora anamorphs that infect the roots of turfgrasses. Phytopathology 80:520-526
24. Lievens B, Brouwer M, Vanachter AC, Cammue B, Thomma B. 2006. Real-time PCR for detection and quantification of fungal and oomycete tomato pathogens in plant and soil samples. Plant Sci 171:155-165
25. Morrison TB,Weis JJ,Wittwer CT. 1998.Quantification of lowcopy transcripts by continuous SYBR Green I monitoring during amplification. BioTechniques 24:954-962.
26. Orlando C, Pinzani P, Pazzaggli M. 1998. Developments in quantitative PCR. Clin Chem Lab Med 36:255-269
27. Schena L, Nigro F, Ippolito A. 2004. Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. Eur J Plant Pathol 110: 893-908
28. Schmittgen TD. 2001. Real-time quantitative PCR. Methods 25:383-385
29. Scott DB, Deacon JW. 1983. Magnaporthe rhizophila sp. nov., a dark mycelial fungus with a Phialophora conidial state, from cereal roots in South Africa. Trans Br Mycol Soc 81:77-81
30. Smiley RW. 1984. Summer patch and necrotic ring spot: newly described components of the fusarium blight syndrome. Proceedings of the New York State Turfgrass Conference 8:75-83.
31. Smiley RW. 1987. The etiologic dilemma concerning patch diseases of bluegrass turfs. Plant Dis 71:774-781
32. Smiley RW. Dernoeden PH, Clarke BB. 2005. Compendium of turfgrass diseases. 3rd ed. St Paul, Minnesota: The American Phytopathological Society Press.
33. Sreedhar L, Kobayashi DY, Bunting TE, Hillman BI, Belanger FC. 1999. Fungal proteinase expression in the interaction of the plant pathogen Magnaporthe poae with its host. Gene 235:121-129
34. Taylor E, Bates J, Kenyon D, Maccaferri M, Thomas J. 2001. Modern molecular methods for characterization and diagnosis of seedborne fungal pathogens. J Phytopathol 83:75-81.
35. Thompson DC, Clarke BB, Kobayashi DY. 1998. Suppression of summer patch by rhizosphere competent bacteria and their establishment on Kentucky bluegrass. Soil Biol Biochem 30:257-263
36. Thompson JD, Gibson TJ, Higgins DG. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882
37. Tredway LP. 2005. First report of summer patch of creeping bentgrass caused by Magnaporthe poae in North Carolina. Plant Dis 89:204
38. Tredway LP. 2006. Genetic relationships among Magnaporthe poae isolates from turfgrass hosts and relative susceptibility of 'Penncross' and 'Penn A-4' creeping bentgrass. Plant Dis 90:1531-1538
39. Vandemark GJ, Grünwald NJ. 2005. Use of real-time PCR to examine the relationship between disease severity in pea and Aphanomyces euteiches DNA content in roots. Eur J Plant Pathol 111:309-316
40. Ward E. 1995. Improved polymerase chain reaction (PCR) detection of Gaeumannomyces graminis including a safeguard against false negatives. Eur J Plant Pathol 101:561-566
41. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR protocols: a guide to methods and applications. San Diego: Academic Press. p 315-322.
42. Winton LM, Stone JK, Watrud LS, Hansen EM. 2002. Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction. Phytopathology 92:112-116