Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphoimaging analyses

Phytopathology

Volumn 92, Issue 8, 2002, Pages 870-876

  Qi, Min ^a   Yang, Yinong ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

Author keywords

Disease resistance; Oryza sativa; Pyricularia grisea; Virulence

Indexed keywords

FUNGI; MAGNAPORTHE; MAGNAPORTHE GRISEA; ORYZA SATIVA; PYRICULARIA;

EID: 0036328098     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.2002.92.8.870     Document Type: Article

References (46)

1. Using GUS expression in a nonpathogenic Fusarium oxysporum strain to measure fungal biomass
2. Scorpion ARMS primers for SNP real-time PCR detection and quantification of Pyrenophora teres
3. The application of real time PCR to the identification, detection, and quantification of Pyrenophora species in barley seed
4. Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea
5. A critical assessment of the validity of ergosterol as an indicator of fungal biomass
6. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citriola in their respective host plant
7. The GUS gene fusion system (Escherichia coli β-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum
8. Use of monoclonal antibodies to detect, quantify, and visualize fungi in soil
9. Identification and differentiation of Tilletia indica and T. walkeri using the polymerase chain reaction
10. Ergosterol determinationin activated sludge and its application as a biochemical marker for monitoring fungal biomass
11. The use of ergosterol as a quantitative measure of the resistance of cultured tissues of Brassica napus ssp. Oleifera to Leptosphaeria maculans
12. PCR assay based on a microsatellite-containing for detection and quantification of Epichoe endophytes in grass tissue
13. Recovery and characterization of spontaneous, selenate-resistant mutants of Magnaporthe grisea, the rice blast pathogen
14. Estimation of amounts of Phytophthora infestans mycelium in leaf tissue by enzyme-linked immunosorbent assay
15. Real time quantitative PCR
16. The polymerase chain reaction and plant disease diagnosis
17. Penetration of hard substrates by a fungus employing enormous turgor pressures
18. Quantification of Verticillium biomass in wilt disease development
19. Enhanced polymerase chain reaction methods for detecting and quantifying Phytophthora infestans in plants
20. Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing β-glucuronidase
21. Use of monoclonal antibodies to determine biomass of Cladosporium fulvum in infected tomato leaves
22. Transformation of Bipolaris sorokiniana with the GUS gene and use for studying fungal colonization of barley roots
23. Fungal- and Plant-specific gene markers to follow the bean anthracnose infection process and normalize a bean chitinase mRNA induction
24. A competitive polymerase chain reaction to quantify DNA of Leptosphaeria maculans during blackleg development in oilseed rape
25. Yeasts have a four-fold variation in ribosomal DNA copy number
26. An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas
27. Impaired fungicide activity in plants blocked in disease resistance signal transduction
28. Detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay
29. Technology for rapid detection, identification, and quantification of rice blast fungus Pyricularia grisea
30. An enzyme-linked immunosorbent assay for quantifying mildew biomass
31. Use of fungal transformants expressing beta-glucuronidase activity to detect infection and measure hyphal biomass in infected plant tissues
32. Measurement of the extraradical mycelium of a vescular-arbuscular mycorrhizal fungus in soil by chitin determination
33. Estimation of mycelia growth of basidiomycetes by means of chin determination
34. Real time PCR fluorescent detection of tomato spotted wilt virus
35. Molecular probes for diagnosis of fungal infections
36. Detection of Clavibacter michiganensis sbusp. Sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system
37. Induced systemic resistance in wounded rice plants
38. Pseudomonas syringae pv. syringae induces systenic resistance to Pyricularia oryzae in rice
39. Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina
40. Relationship of soil ergosterol concentration and fungal biomass
41. Dynamics of fungal pathogens in host plant tissues
42. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea
43. Disturbed correlation between fungal biomass and β-glucuronidase activity in infections of Arabidopsis thaliana with transgenic Alternaria brassicicola
44. A tool for monitoring Trichoderma harzianum: I. Transformation with the GUS gene by protoplast technology
45. Applications of PCR in fungal-plant interactions
46. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay