SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

Crop Protection

Volumn 33, Issue , 2012, Pages 1-6

  López Mondéjar, Rubén ^a   Beaulieu, Robert ^a   Ros, Margarita ^a   Pascual, José Antonio ^a  

^a RESEARCH GROUP ON QUALITY   (Spain)

Author keywords

Fusarium oxysporum; Fusarium wilt; Greenhouse nursery; Real time TaqMan PCR; SCAR marker

Indexed keywords

DETECTION METHOD; FUNGUS; GENETIC MARKER; NURSERY GROUND; PATHOGENICITY; POLYMERASE CHAIN REACTION; SEEDLING; SENSITIVITY ANALYSIS;

CUCUMIS; FUNGI; FUSARIUM; FUSARIUM OXYSPORUM;

EID: 83055163666     PISSN: 02612194     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cropro.2011.10.009     Document Type: Article

References (36)

1. Al-Sadi A.M., Al-Said F.A., Al-Jabri A.H., Al-Mahmooli I.H., Al-Hinai A.H., de Cock A.W.A.M. Occurrence and characterization of fungi and oomycetes transmitted via potting mixtures and organic manures. Crop Prot. 2011, 30:38-44.
2. Atkins S.D., Clark I.M., Sosnowska D., Hirsch P.R., Kerry B.R. Detection and quantification of Plectosphaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media and baiting. Appl. Environ. Microbiol. 2003, 69:4788-4793.
3. Baayen R.P. Diagnosis and detection of host-specific forms of Fusarium oxysporum. EPPO Bull. 2000, 30:489-491.
4. Castle A., Speranzini D., Rghei N., Alm G., Rinker D., Bisset J. Morphological and molecular identification of Trichoderma isolates on North American Mushrooms Farms. Appl. Environ. Microbiol. 1998, 64:133-137.
5. Castrillo L.A., Vandenberg J.D., Wraight S.P. Strain-specific detection of introduced Beauveria bastiana in agricultural fields by use of sequence-characterized amplified region marker. J.Invertebr. Pathol. 2003, 82:75-83.
6. Cordier C., Edel-Ilermann V., Martin-Laurent F., Blal B., Steinberg C., Alabouvette C. SCAR-based real-time PCR to identify a biocontrol strain (T1) of Trichoderma atroviride and study its population dynamics in soils. J.Microbiol. Methods 2007, 68:60-68.
7. Dalzoto P.R., Glienke-Blanco C., Kava-Cordeiro V., Araujo W.L., Azevedo J.L. RAPD analyses of recombination processes in the entomopathogenic fungus Beauveria bassiana. Mycol. Res. 2003, 107:1069-1074.
8. Di Pietro A., Madrid M.P., Caracuel Z., Delgado-Jarana J., Roncero M.I.G. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Mol. Plant Pathol. 2003, 4:315-326.
9. Doyle J.J., Doyle J.L. Arapid DNA isolation procedure for small quantities of fresh leaf tissues. Phytochem. Bull. 1987, 19:11-15.
10. Filion M., St-Arnaud M., Jabaji-Hare S.H. Direct quantification of fungal DNA from soil substrate using real-time PCR. J.Microbiol. Methods 2003, 53:67-76.
11. Fravel D., Olivain C., Alabouvette C. Fusarium oxysporum and its biocontrol. New Phytol. 2002, 157:493-502.
12. Fredricks D.N., Smith C., Meier A. Comparison of Six DNA extraction methods for Recovery of fungal DNA as assessed by quantitative PCR. J. Clin. Microbiol. 2005, 43:5122-5128.
13. Friesen T.L., Stukenbrock E.H., Liu Z., Meinhardt S., Ling H., Faris J.D. Emergence of a new disease as a result of interspecific virulence gene transfer. Nat. Genet. 2006, 38:953-956.
14. Gómez J., Tello J.C. Presencia de la raza 1-2 de Fusarium oxysporum f. sp. melonis en Almería. Bol. San. Veg. Plagas 2000, 26:27-33.
15. Gordon T.R., Okamoto D., Jacobson D.J. Colonization of muskmelon and nonsusceptible crops by Fusarium oxysporum f. sp. melonis and other species of Fusarium. Phytopathology 1989, 79:1095-1100.
16. James R.L., Dumroese R.K., Wenny D.L. Fusarium disease of conifer seedlings. Proceedings of the First Meeting of IUFRO Working Party S2.07-09 (Diseases and Insects in Forest Nurseries). Forestry Canada, Pacific and Yukon Region 1991, 181-190. Information Report BC-X-331. J.R. Sutherland, S.G. Glover (Eds.).
17. Jiménez-Gascó M.M., Jiménez-Díaz R.M. Development of a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5 and 6. Phytopathology 2003, 93:200-209.
18. Joosten M.H.A.J., Cozijinsen T.J., de Wit P.J.G.M. Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulance gene. Nature 1994, 367:384-386.
19. Komada H. Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Rev. Plant Prot. Res. 1975, 8:114-125.
20. López-Mondéjar R., Antón A., Raidl S., Ros M., Pascual J.A. Quantification of the biocontrol agent Trichoderma harzianum with real-time TaqMan PCR and its potential extrapolation to the hyphal biomass. Bioresour. Technol. 2010, 110:2888-2891.
21. Lievens B., Thomma B.P.H.J. Recent developments in pathogen detection arrays: implications for fungal plant pathogens and use in practice. Phytopathology 2005, 95:1374-1380.
22. Lievens B., Rep M., Thomma B.P.H.J. Recent development in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Manag. Sci. 2008, 64:781-788.
23. Lin Y.H., Chen K.S., Chang J.Y., Wan Y.L., Hsu C.C., Huang J.W., Chang P.F.L. Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan. New Biotechnol. 2010, 27:409-418.
24. McCartney H.A., Foster S.J., Fraaije B.A., Ward E. Molecular diagnostics for fungal plant pathogens. Pest Manag. Sci. 2003, 59:129-142.
25. Nash S., Snyder W.C. Quantitative estimations by plate count of propagules of the bean root Fusarium in field soil. Phytopathology 1962, 52:567-572.
26. Pasquali M., Piatti P., Gullino M.L., Garibaldi A. Development of a real-time polymerase chain reaction for the detection of Fusarium oxysporum f. sp. basilici from Basil seed and roots. J.Phytopathol. 2006, 154:632-636.
27. Schäfer C., Wöstemeyer J. Random-primer-dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Leptophaeria maculans). J.Phytopathol. 1992, 136:124-136.
28. Schena L., Nigro F., Ippolito A., Gallitelli D. Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi. Eur. J. Plant Pathol. 2004, 110:893-908.
29. Suárez-Estrella F., Vargas-García M.C., López M.J., Moreno J. Survival of Fusarium oxysporum f. sp. melonis on plant waste. Crop Prot. 2004, 23:127-133.
30. Vakalounakis D.J., Doulis A.G., Klironomou E. Characterization of Fusarium oxysporum f. sp. radicis-cucumerinum attacking melon under natural conditions in Greece. Plant Pathol. 2005, 54:339-346.
31. Validov S.Z., Kamilova F.D., Lugtenberg B.J.J. Monitoring of pathogenic and non-pathogenic Fusarium oxysporum strains during tomato plant infection. Microb. Biotechnol. 2011, 4:82-88.
32. Voigt K., Schleier S., Wöstemeyer J. RAPD-based molecular probes for the blackleg fungus Leptosphaeria maculans (Phoma lingam): evidences for pathogenicity group-specific sequences in the fungal genomes. J.Phytopathol. 1998, 146:567-576.
33. Waller P., Thornton C.R., Farley D., Groenhof A.C. Pathogens and other fungi in growing media constituents. Acta Hortic. 2008, 779:361-365.
34. Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 1990, 18:6531-6535.
35. Wilson I.G. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 1997, 63:3741-3751.
36. Zhang Z., Zhang J., Wang Y., Zheng X. Molecular detection of Fusarium oxysporum f. sp. niveum and Mycosphaerella melonis in infected plant tissues and soil. FEMS Microbiol. Lett. 2005, 248:39-47.