Quantification of conidial density of aspergillus flavus and a parasiticus in soil from almond orchards using real-time PCR

Journal of Applied Microbiology

Volumn 106, Issue 5, 2009, Pages 1649-1660

  Luo, Y ^a   Gao, W ^b   Doster, M ^a   Michailides, T J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b INSTITUTE OF MEDICINAL PLANT DEVELOPMENT   (China)

Author keywords

Aflatoxin; Fungi; Internal transcribed spacer; Quantification; Real time PCR; Soil

Indexed keywords

ASPERGILLUS; DNA SEQUENCES; POLYMERASE CHAIN REACTION; SOILS; TRANSCRIPTION;

ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; DNA EXTRACTION; FUNGAL SPECIES; INTERNAL TRANSCRIBED SPACERS; PARASITICUS; QUANTIFICATION; REAL-TIME PCR; REAL-TIME PCR ASSAY; SPECIFIC PRIMERS;

AFLATOXINS;

INTERNAL TRANSCRIBED SPACER; PRIMER DNA;

BIOASSAY; DNA; EXTRACTION; FUNGUS; ORCHARD; PATHOGEN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS;

ALMOND; ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; CONIDIUM; DENSITY; DNA EXTRACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; ORCHARD; REAL TIME POLYMERASE CHAIN REACTION; SOIL;

ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; FUNGI; PRUNUS DULCIS;

EID: 64149091561     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04132.x     Document Type: Article

References (32)

1. Angle, J.S., Dunn, K.A. Wagner, G.H. (1982) Effect of cultural practices on the soil population of Aspergillus flavus and Aspergillus parasiticus. Soil Sci Soc Am J 46, 301 304.
2. Chu, F.S. (1991) Mycotoxins: food contamination, mechanisms, carcinogenic potential and preventative measures. Mutat Res 259, 291 306.
3. Cotty, P.J. (1994) Influence of field application of an atoxigenic strain of Aspergillus flavus on the populations of A. flavus infecting cotton bolls and on the aflatoxin content of cottonseed. Phytopathology 84, 1270 1277.
4. Cotty, P.J. (1997) Aflatoxin-producing potential of communities of Aspergillus section Flavi from cotton producing areas in the United States. Mycol Res 101, 698 704.
5. Cullen, D.W., Lees, A.K., Toth, I.K. Duncan, J.M. (2001) Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in soil and on potato tubers. Eur J Plant Pathol 107, 387 398.
6. Cullen, D.W., Toth, I.K., Pitkin, Y., Boonham, N., Walsh, K., Barker, I. Lees, A.K. (2005) Use of quantitative molecular diagnostic assays to investigate Fusarium dry rot in potato stocks and soil. Phytopathology 95, 1462 1471.
7. Das, M.K., Ehrlich, K.C. Cotty, P.J. (2008) Use of pyrosequencing to quantify incidence of a specific Aspergillus flavus strain within complex fungal communities associated with commercial cotton crops. Phytopathology 98, 282 288.
8. Dorner, J.W. Horn, B.W. (2007) Separate and combined applications of nontoxigenic Aspergillus flavus and A. parasiticus for biocontrol of aflatoxin in peanuts. Mycopathologia 163, 215 223.
9. Dorner, J.W. Horner, B.W. (2004) Effect of nontoxigenic strains of Aspergillus flavus and A. parasiticus applied separately and in combination on preharvest aflatoxin contamination of peanuts. Mycopathologia 157, 450.
10. Doster, M.A. Michailides, T.J. (1994) Aspergillus molds and aflatoxins in pistachio nuts in California. Phytopathology 84, 583 590.
11. Ehrlich, K.C. Cotty, P.C. (2004) An isolate of Aspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene. Appl Microb Biotech 65, 473 478.
12. Ehrlich, K.C., Yu, J. Cotty, P.J. (2005) Aflatoxin biosynthesis gene clusters and flanking regions. J Appl Microb 99, 518 527.
13. Filion, M., St-Arnaud, M. Jabaji-Hare, S.H. (2003a) Direct quantification of fungal DNA from soil substrate using realtime PCR. J Microbiol Methods 53, 67 76.
14. Filion, M., St-Arnaud, M. Jabaji-Hare, S.H. (2003b) Quantification of Fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. Phytopathology 93, 229 235.
15. Frostegard, A., Courtois, S., Ramisse, V., Clerc, S., Bernillon, D., LeGall, F., Jeannin, P., Nesme, X. et al. (1999) Quantification of bias related to the extraction of DNA directly from soils. Appl Environ Microbiol 65, 5409 5420.
16. Horn, B.W. (2007) Biodiversity of Aspergillus section Flavi in the United States: a review. Food Addit Contam 24, 1088 1101.
17. Horn, B.W., Greene, R.L. Dorner, J.W. (1995) Effect of corn and peanut cultivation on soil populations of Aspergillus flavus and A. parasiticus in southwestern Georgia. Appl Environ Microbiol 61, 2472 2475.
18. Jaime-Garcia, R. Cotty, P.J. (2004) Aspergillus flavus in soils and corncobs in South Texas: implications for management of aflatoxins in corn-cotton rotations. Plant Dis 88, 1366 1371.
19. Kanbe, T., Yamaki, K. Kikuchi, A. (2002) Identification of the pathogenic Aspergillus species by nested PCR using a mixture of specific primers to DNA topoisomerase II gene. Microbiol Immunol 46, 841 848.
20. Luo, Y., Ma, Z. Michailides, T.J. (2007a) Quantification of allele E198A conferring high benzimidazole-resistance in Monilinia fructicola populations from California stone fruit orchards using real-time PCR. Pest Manag Sci 63, 1178 1184.
21. Luo, Y., Ma, Z., Reyes, H.C., Morgan, D.P. Michailides, T.J. (2007b) Using real-time PCR to survey frequency of azoxystrobin-resistant allele A143 in Alternaria populations from almond and pistachio orchards in California. Pestic Biochem Physiol 88, 328 336.
22. Ophel-Keller, K., Mckay, A., Hartley, D., Herdina Curran, J. (2008) Development of a routine DNA-based testing service for soilborne diseases in Australia. Australas Plant Pathol 37, 243 253.
23. Pavón, C.F., Babadoost, M. Lambert, K.N. (2008) Quantification of Phytophthora capsici oospores in soil by sieving-centrifugation and real-time polymerase chain reaction. Plant Dis 92, 143 149.
24. Peterson, S.W., Horn, B.W., Ito, Y. Goto, T. (2000) Genetic variation and aflatoxin production in Aspergillus tamarii and A. caelatus. In Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification ed. Samson, R.A. Pitt, J.I. pp. 447 458 Amsterdam : Harwood Academic.
25. Seifert, K.A. Lévesque, C.A. (2004) Phylogeny and molecular diagnosis of mycotoxigenic fungi. Eur J Plant Pathol 110, 449 471.
26. Steffan, R.J., Goksoyr, J., Bej, A.K. Atlas, R.M. (1988) Recovery of DNA from soils and sediments. Appl Environ Microbiol 54, 2908 2915.
27. Stults, J.R., Snoeyenbos-West, O., Methe, B., Lovley, D. Chandler, D.P. (2001) Application of the 50 fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments. Appl Environ Microbiol 67, 2781 2789.
28. Van de Graaf, P., Lees, A.K., Cullen, D.W. Duncan, J.M. (2003) Detection and quantification of Spongospora subterranea f. sp. subterranean in soil, water and plant tissue samples using real-time PCR. Eur J Plant Pathol 109, 589 597.
29. Wang, Y., Zhang, W., Wang, Y. Zheng, X. (2006) Rapid and sensitive detection of Phytophthora sojae in soil and infected soybeans by species-specific polymerase chain reaction assays. Phytopathology 96, 1315 1321.
30. Watson, R.J. Blackwell, B. (2000) Purification and characterization of a common soil component which inhibits the polymerase chain reaction. Can J Microb 46, 633 642.
31. White, T.J., Bruns, T.D., Lee, S. Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In PCR Protocols ed. Innis, M.A., Gelfrand, D.H., Sninsky, J.J. White, T.J. pp. 315 322. San Diego, CA : Academic Press.
32. Yabe, K. Nakajima, H. (2004) Enzyme reactions and genes in aflatoxin biosynthesis. Appl Microbiol Biotechnol 64, 745 755.