Improved real-time PCR assay for detection of the quarantine potato pathogen, Synchytrium endobioticum, in zonal centrifuge extracts from soil and in plants

European Journal of Plant Pathology

Volumn 126, Issue 1, 2009, Pages 129-133

  van Gent Pelzer, Marga P E ^a   Krijger, Marjon ^a   Bonants, Peter J M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Detection; Identification; Potato wart disease; TaqMan PCR

Indexed keywords

CYTOCHROME; DETECTION METHOD; FUNGAL DISEASE; GENE; IDENTIFICATION METHOD; MITOCHONDRIAL DNA; PATHOTYPE; SUBSTRATE; TUBER;

BACTERIA (MICROORGANISMS); SOLANUM TUBEROSUM; SYNCHYTRIUM ENDOBIOTICUM;

EID: 71149115482     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-009-9522-3     Document Type: Article

References (16)

1. Abdullahi, I., Koerbler, M., Stachewicz, H., & Winter, S. (2005). The 18S rDNA sequence of Synchytrium endobioticum and its utility in microarrays for the simultaneous detection of fungal and viral pathogens of potato. Applied Microbiology and Biotechnology, 68(3), 368-375.
2. Hampson, M. C. (1985). Sequence of events in the germination of the resting spore of Synchytrium endobioticum, European pathotype 2, the causal agent of potato wart disease. Canadian Journal of Botany, 64, 2144-2150.
3. Hampson, M. C. (1993). History, biology and control of potato wart disease in Canada. Canadian Journal of Plant Pathology, 15, 223-244.
4. Hampson, M. C., Yang, A. F., & Bal, A. K. (1994). Ultrastructure of Synchytrium endobioticum resting spores and enhancement of germination using snails. Mycologia, 86, 733-740.
5. Hendrickx, G. (1995). An automatic apparatus for extracting freeliving nematode stages from soil. Nematologica, 41, 308.
6. Klerks, M. M., Zijlstra, C., & van Bruggen, A. H. C. (2004). Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157: H7, and introduction of a general internal amplification control. Journal of Microbiological Methods, 59, 337-349.
7. Laidlaw, W. M. R. (1985). A method for the detection of the resting sporangia of potato wart disease (Synchytrium endobioticum) in the soil of old outbreak sites. Potato Research, 28, 223-232.
8. Levesque, C. A., de Jong, S. N., Ward, L. J., & DeBoer, S. H. (2001). Molecular phylogeny and detection of Synchytrium endobioticum, causal agent of potato wart. Canadian Journal Plant Pathology, 23, 200-201.
9. Levesque, C. A., Ward, L. J., de Jong, S. N., De Boer, S. N., van den Boogert, P. H. J. F., & Baayen, R. P. (2002). A new soil assay for Synchytrium endobioticum the causal agent of potato wart. Phytopathology, 92, S46.
10. Niepold, F., & Stachewicz, H. (2004). PCR-detection of Synchytrium endobioticum (Schilb.) Perc. Zeitschrift-fuer-Pflanzenkrankheiten-und-Pflanzenschutz. Journal of Plant Diseases and Protection, 111(4), 313-321.
11. Pratt, M. A. (1976). A wet-sieving and flotation technique for the detection of resting sporangia of Synchytrium endobioticum in soil. Annals of Applied Biology, 82, 21-29.
12. van den Boogert, P. H. J. F., van Gent-Pelzer, M. P. E., Bonants, P. J. M., de Boer, S. H., Wander, J. G. N., Levesque, C. A., et al. (2005). Development of PCR-based detection methods for the quarantine phytopathogen Synchytrium endobioticum, causal agent of potato wart disease. European Journal of Plant Pathology, 113(1), 47-57.
13. van Leeuwen, G. C. M., Wander, J. G. N., Lamers, J., Meffert, J. P., van den Boogert, P. H. J. F., & Baayen, R. P. (2005). Direct examination of soil for sporangia of Synchytrium endobioticum using chloroform, calcium chloride and zinc sulphate as extraction reagents. EPPO Bulletin, 35, 25-31.
14. Wander, J. G. N., van den Berg, W., van den Boogert, P. H. J. F., Lamers, J. G., van Leeuwen, G. C. M., Hendrickx, G., et al. (2007). Use of a novel technique, the Hendrickx centrifuge, for extracting winter sporangia of Synchytrium endobioticum from soil. European Journal of Plant Pathology, 119, 165-174.
15. Weller, S. A., Elphinstone, J. G., Smith, N. C., Boonham, N., & Stead, D. E. (2000). Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Applied Environmental Microbiology, 66, 2853-2858.
16. Zelya, A. G., & Melnik, P. A. (1998). Detection methods for Synchytrium endobioticum. Bulletin OEPP/EPPO Bulletin, 28(4), 543-544.