Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells

Journal of Microbiological Methods

Volumn 60, Issue 3, 2005, Pages 315-323

  Wolffs, Petra ^a,b   Norling, Börje ^c   Rådström, Peter ^a  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF GUELPH   (Canada)

^c Quintessence Research AB   (Sweden)

Author keywords

Dead cells; False positive results; Floatation; Flotation; Real time PCR; Yersinia enterocolitica

Indexed keywords

ANALYTICAL ERROR; ARTICLE; CELL COUNT; CELL DEATH; CELL VIABILITY; CHICKEN; DNA DEGRADATION; DNA DETERMINATION; FOOD ANALYSIS; LABORATORY DIAGNOSIS; MEAT; MICROORGANISM DETECTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RISK ASSESSMENT;

ANIMALS; CELL DEATH; CENTRIFUGATION, DENSITY GRADIENT; CHICKENS; COLONY COUNT, MICROBIAL; DNA; DNA, BACTERIAL; FALSE POSITIVE REACTIONS; FOOD MICROBIOLOGY; MEAT; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; SWINE; YERSINIA ENTEROCOLITICA; YERSINIA INFECTIONS;

GALLUS GALLUS; YERSINIA; YERSINIA ENTEROCOLITICA;

EID: 12144272204     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.10.003     Document Type: Article

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