Optimization of a real-time PCR assay to quantitate airborne fungi collected on a gelatin filter

Journal of Bioscience and Bioengineering

Volumn 109, Issue 1, 2010, Pages 83-88

  Yamamoto, Naomichi ^a,b   Kimura, Minoru ^c   Matsuki, Hideaki ^a   Yanagisawa, Yukio ^d  

^a TOKAI UNIVERSITY   (Japan)

^b JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

^c School of Medicine   (Japan)

^d Graduate School of Frontier Sciences   (Japan)

Author keywords

Fungal spore; Fungi; Gelatin filter; qPCR; Quantification

Indexed keywords

AIRBORNE FUNGAL CONCENTRATION; AIRBORNE FUNGI; CULTURE-BASED METHODS; DETECTION SENSITIVITY; DETECTION TECHNIQUE; DILUTION RATIO; DNA EXTRACTION; DNA EXTRACTS; DNA YIELDS; FUNGAL CELLS; FUNGAL DNA; FUNGAL SPORE; FUNGAL SPORES; GELATIN FILTERS; LIMIT OF QUANTIFICATIONS; OPTIMIZED CONDITIONS; ORDERS OF MAGNITUDE; OUTDOOR ENVIRONMENT; REAL-TIME PCR; REAL-TIME PCR ASSAY;

AMPLIFICATION; CELL CULTURE; DNA; FUNGI; GENES; OPTIMIZATION;

NUCLEIC ACIDS;

FUNGAL DNA;

AIRBORNE FUNGUS; ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; GENE AMPLIFICATION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

AIR MICROBIOLOGY; AIR POLLUTION, INDOOR; ASPERGILLUS NIGER; DNA, FUNGAL; ENVIRONMENTAL MONITORING; FILTRATION; FUNGI; GELATIN; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 18S; SPORES, FUNGAL;

FUNGI;

EID: 72249108153     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.06.015     Document Type: Article

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