Production of Recombinant Disulfide-Rich Venom Peptides for Structural and Functional Analysis via Expression in the Periplasm of E. coli

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Klint, Julie K ^a   Senff, Sebastian ^a   Saez, Natalie J ^a   Seshadri, Radha ^a   Lau, Ho Yee ^a   Bende, Niraj S ^a   Undheim, Eivind A B ^a   Rash, Lachlan D ^a   Mobli, Mehdi ^a   King, Glenn F ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; CARBON 13; CENTIPEDE VENOM; DISULFIDE RICH VENOM PEPTIDE; HYBRID PROTEIN; NITROGEN 15; SCORPION VENOM; SEA ANEMONE TOXIN; SPIDER VENOM; UNCLASSIFIED DRUG; VENOM;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; DISULFIDE BOND; ESCHERICHIA COLI; EXPRESSION VECTOR; INTERMETHOD COMPARISON; ISOTOPE LABELING; MOLECULAR SIZE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PEPTIDE SYNTHESIS; PH; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INDUCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY; STRUCTURE ANALYSIS; TEMPERATURE;

ANIMALS; BUFFERS; DISULFIDES; ESCHERICHIA COLI; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; PEPTIDES; PERIPLASM; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE;

EID: 84877129926     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0063865     Document Type: Article

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