An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization

Protein Expression and Purification

Volumn 51, Issue 1, 2007, Pages 102-109

  Fang, Lei ^a   Jia, Kun Zhi ^a   Tang, Ya Lan ^a   Ma, Ding Yuan ^a   Yu, Mei ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Fusion protein cleavage; High yield and solubility; His6 TEV; Lower temperature; Molecular chaperones

Indexed keywords

CHAPERONE; CHAPERONIN; ESCHERICHIA COLI PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; OLIGOPEPTIDE; PEPTIDYLPROLYL ISOMERASE; PROTEINASE; TEV PROTEASE; TRIGGER FACTOR, E COLI;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME INDUCTION; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; SOLUBILITY; TEMPERATURE;

CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENDOPEPTIDASES; ENZYME INDUCTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GROEL PROTEIN; GROES PROTEIN; HISTIDINE; ISOPROPYL THIOGALACTOSIDE; MOLECULAR CHAPERONES; OLIGOPEPTIDES; PEPTIDYLPROLYL ISOMERASE; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TEMPERATURE;

ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 33751408438     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.003     Document Type: Article

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