Microvolume spectrophotometric and fluorometric determination of protein concentration

Current Protocols in Protein Science

Volumn , Issue SUPPL. 55, 2009, Pages

  Desjardins, Philippe ^a   Hansen, Joel B ^a   Allen, Michael ^a  

^a Thermo Fisher Scientific   (United States)

Author keywords

Microsample; Microsample analysis; Microvolume; Microvolume analysis; Microvolume fluorospectrometer; Microvolume spectrophotometer; Microvolume spectroscopy; Protein concentration

Indexed keywords

CLINICAL PROTOCOL; COLORIMETRY; COMPUTER PROGRAM; FLUOROMETRY; HYDROPHILICITY; HYDROPHOBICITY; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; QUALITY CONTROL; REVIEW; SENSITIVITY ANALYSIS; SPECTROPHOTOMETRY; VOLUMETRY; ARTICLE; FLUORESCENCE ANALYSIS; MICROVOLUME SPECTROPHOTOMETRY; MOLECULAR WEIGHT; SURFACE TENSION;

FLUOROMETRY; PROTEINS; SAMPLE SIZE; SPECTROPHOTOMETRY;

BOVINE SERUM ALBUMIN; IMMUNOGLOBULIN G;

EID: 61349139504     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0310s55     Document Type: Review

References (8)

1. Aitken, A. and Learmonth, M. 1996. Protein determination by UV absorption. In Protein Protocols Handbook (J.M. Walker, ed). Springer, Secaucus, N.J.
2. Bradford,M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Lowry, O.H., Rosebrough, N.J., Farr, A., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
4. Ohnishi, S.T. and Barr, J.K. 1978. A simplified method of quantitating protein using the biuret and phenol reagents. Anal. Biochem. 86:193- 200.
5. Reisner, A.H., Nemes, P., and Bucholtz, C. 1975. Use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels. Anal. Biochem. 64:509-516.
6. Simonian,M.H. and Smith, J.A. 2006. Spectrophotometric and colorimetric determination of protein concentration. Curr. Protoc. Mol. Biol. 10:1A.17.
7. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
8. Stoscheck, C.M. 1990. Quantitation of protein. Meth. Enzymol. 182:50-68.