Practical protocols for production of very high yields of recombinant proteins using Escherichia coli

Protein Science

Volumn 18, Issue 5, 2009, Pages 936-948

  Sivashanmugam, Arun ^a   Murray, Victoria ^a   Cui, Chunxian ^a   Zhang, Yonghong ^a   Wang, Jianjun ^a,b   Li, Qianqian ^a,b  

^a WAYNE STATE UNIVERSITY   (United States)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bacterial expression; Escherichia coli; Expression condition and media; High cell density; Recombinant protein

Indexed keywords

APOLIPOPROTEIN A1; APOLIPOPROTEIN E; BACTERIAL DNA; RAP PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL DENSITY; ESCHERICHIA COLI; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

APOLIPOPROTEIN A-I; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; ISOPROPYL THIOGALACTOSIDE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TEMPERATURE; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA;

EID: 65649135871     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.102     Document Type: Article

References (29)

1. Swartz JR (2001) Advances in Escherichia coli production of therapeutic proteins. Curr Opin Biotechnol 12:195-201.
2. Hewitt L, McDonnell JM (2004) Screening and optimizing protein production in E. coli methods. Mol Biol 278:1-16.
3. Jana S, Deb JK (2005) Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol 67:289-298. (Pubitemid 40684730)
4. Peti W, Page R (2006).
5. Structural Genomics Consortium, et al. (2008) Protein production and purification. Nat Methods 5:135-146.
6. Riek R, Fiaux J, Bertelsen EB, Horwich AL, Wuthrich K (2002) Solution NMR techniques for large molecular and supramolecular structures, J Am Chem Soc 124:12144-12153. (Pubitemid 35154881)
7. Tugarinov V, Hwang PM, Kay LE (2004) Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins. Annu Rev Biochem 73:107-146. (Pubitemid 39050365)
8. Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 10:411-421. (Pubitemid 29457264)
9. Studier FW (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41:207-234.
10. Tyler RC, Sreenath HK, Singh S, Aceti DJ, Bingman CA, Markley JL, Fox BG (2005) Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination. Protein Expr Purif 40:268-278. (Pubitemid 40335909)
11. Sreenath HK, Bingman CA, Buchan BW, Seder KD, Burns BT, Geetha HV, Jeon WB, Vojtik FC, Aceti DJ, Frederick RO, Phillips GN, Jr, Fox BG (2005). Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. Protein Expr Purif 40:256-267.
12. Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 72:211-222. (Pubitemid 44273045)
13. Miroux B, Walker JE (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260:289-298. (Pubitemid 26254109)
14. Sahdev S, Khattar SK, Saini KS (2008) Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies. Mol Cell Biochem 307:249-264. (Pubitemid 50006245)
15. Gschaedler A, Robas N, Boudrant J, Branlant C (1999) Effects of pulse addition of carbon sources on continuous cultivation of Escherichia coli containing a recombinant E. coli gapA gene. Biotechnol Bioeng 63:712-720. (Pubitemid 29200406)
16. Chen HC, Hwang CF, Mou DG (1992) High-density Escherichia coli cultivation process for hyperexpression of recombinant porcine growth hormone. Enzyme Microb Technol 14:321-326. (Pubitemid 23638482)
17. Hannig G, Makrides SC (1998) Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol 16:54-60. (Pubitemid 28055472)
18. Cai M, Huang Y, Sakaguchi K, Clore GM, Gronenborn AM, Craigie R (1998) An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli. J Biomol NMR 11:97-102. (Pubitemid 128510229)
19. Marley J, Lu M, Bracken C (2001) A method for efficient isotopic labeling of recombinant proteins. J Biomol NMR 20:71-75. (Pubitemid 32519656)
20. Zhang Y, Chen J, Wang JA (2008) Complete backbone spectral assignment of human apolipoprotein E. Biomol NMR Assign 2:207-210.
21. Yang Y, Wang J (2007) A complete NMR spectral assignment of lipid-free mouse apolipoprotein A-I C-terminal truncation mutant, apoAI(1-216). Biomol NMR Assign 1:109-111.
22. Ren X, Yang Y, Neville T, Sparks D, Wang J (2007) A complete backbone spectral assignment of human apoAI/preβHDL particle. Biomol NMR Assign 1:69-71.
23. Sivashanmugam A, Wang J (in press) NMR structure of the complete receptor-binding domain of human apolipoprotein E. J Biol Chem.
24. Fisher CA, Wang J, Francis GA, Sykes BD, Kay CM, Ryan RO (1997) Bacterial overexpression, isotope enrichment, and NMR analysis of the N-terminal domain of human apolipoprotein E. Biochem Cell Biol 75:45-53. (Pubitemid 127733127)
25. Ren X, Zhao L, Sivashanmugam A, Miao Y, Korando L, Yang Z, Reardon CA, Getz GS, Brouillette CG, Jerome WG, Wang J (2005) Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determination. Biochemistry 44:14907-14919. (Pubitemid 41612270)
26. Cui C, Chen J, Zhao W, Wang J, Li Q (2006) Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems. Protein Expr Purif 50:74-81. (Pubitemid 44648744)
27. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277-293.
28. Johnson BA, Blevins RA (1994) NMR view: a computer program for the visualization and analysis of NMR data. J Biomol NMR 4:603-614.
29. Peti W, Page R (2007) Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Protein Expr Purif 51:1-10. (Pubitemid 46636328)