Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteins

Protein Expression and Purification

Volumn 50, Issue 1, 2006, Pages 68-73

  Kurz, Mareike ^a   Cowieson, Nathan P ^a   Robin, Gautier ^a   Hume, David A ^a   Martin, Jennifer L ^a   Kobe, Bostjan ^a   Listwan, Pawel ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

E. coli; Fusion protein; Histidine; Protein purification; Protein solubility; Proteolytic cleavage; Recombinant protein expression in E. coli; TEV (tobacco etch virus) protease

Indexed keywords

ANTIGEN; C21ORF19 LIKE PROTEIN, MOUSE; C21ORF19-LIKE PROTEIN, MOUSE; MJD PROTEIN, MOUSE; NUCLEAR PROTEIN; OSR1 PROTEIN, MOUSE; PC3.1 ANTIGEN; PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; SERF2 PROTEIN, MOUSE; TEV PROTEASE; TRANSCRIPTION ELONGATION FACTOR; TRANSCRIPTION FACTOR; UPSTREAM STIMULATORY FACTOR; USF1 PROTEIN, MOUSE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CODON; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POTYVIRUS; PROTEIN ENGINEERING; SOLUBILITY; TOBACCO; VIROLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS; CODON; ENDOPEPTIDASES; GENETIC VECTORS; MICE; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; POTYVIRUS; PROTEIN ENGINEERING; PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY; TOBACCO; TRANSCRIPTION FACTORS; TRANSCRIPTIONAL ELONGATION FACTORS; UPSTREAM STIMULATORY FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 33750489405     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.05.006     Document Type: Article

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