The optimization of in vitro high-throughput chemical lysis of Escherichia coli. Application to ACP domain of the polyketide synthase ppsC from Mycobacterium tuberculosis

Journal of Structural and Functional Genomics

Volumn 11, Issue 1, 2010, Pages 41-49

  Listwan, Pawel ^a   Pédelacq, Jean Denis ^a,b,c   Lockard, Meghan ^a   Bell, Carolyn ^a   Terwilliger, Thomas C ^a   Waldo, Geoffrey S ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITÉ PAUL SABATIER   (France)

Author keywords

Automation; Cell lysis; High throughput screening; Protein solubility; Robotics; Split GFP

Indexed keywords

ACYL CARRIER PROTEIN; ADJUVANT; GREEN FLUORESCENT PROTEIN; LYSOZYME; POLYKETIDE SYNTHASE;

ACCURACY; ARTICLE; BACTERIOLYSIS; CELL CULTURE; CELL DISRUPTION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOLYSIS; ESCHERICHIA COLI; FREEZE THAWING; GENE SEQUENCE; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; METHODOLOGY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; ROBOTICS; TEMPERATURE; ULTRASOUND;

ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; MYCOBACTERIUM TUBERCULOSIS; POLYKETIDE SYNTHASES; PROTEINS; SOLUBILITY; TEMPERATURE; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS;

EID: 77951296511     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10969-009-9077-8     Document Type: Article

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