A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins

Gene

Volumn 186, Issue 1, 1997, Pages 55-60

  Zheng, Chao Feng ^a   Simcox, Timothy ^a   Xu, Li ^a   Vaillancourt, Peter ^a  

^a Stratagene   (United States)

Author keywords

Affinity purification; cAMP dependent protein kinase A; Expression vector; Recombinant fusion protein

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALMODULIN BINDING PROTEIN; CYCLIC AMP; EGTAZIC ACID; HYBRID PROTEIN; MYOSIN LIGHT CHAIN KINASE; PHOSPHORUS 32; PHOSPHOTRANSFERASE; THROMBIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CELL LYSATE; ESCHERICHIA COLI; EXPRESSION VECTOR; ISOTOPE LABELING; MOLECULAR CLONING; NONHUMAN; ONCOGENE C JUN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CALMODULIN-BINDING PROTEINS; CLONING, MOLECULAR; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ESCHERICHIA COLI; GENETIC VECTORS; HISTONES; ISOTOPE LABELING; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PHOSPHORUS RADIOISOTOPES; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; THROMBIN;

ESCHERICHIA COLI;

EID: 0031579473     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00680-4     Document Type: Article

References (20)

1. Blanar, M.A. and Rutter, W.J. (1992) Interaction cloning: identification of a helix-loop-helix leucine zipper protein that interacts with c-fos. Science 256, 1014-1017.
2. Derijard, B., Hibi, M., Wu, I.-H., Barrett, T., Su, B., Deng, T., Karin, M. and Davis, R.J. (1994) JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76, 1025-1037.
3. Gray, M.R., Colot, H.V., Guarente, L. and Rosbash, M. (1982) Open reading frame cloning: identification, cloning and expression of open reading frame DNA. Proc. Natl. Acad. Sci. USA 79, 6598-6692.
4. Guan, C., Li, P., Riggs, P.D. and Inouye, H. (1988) Vectors that facilitate the expression and purification of foregn peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67, 21-30.
5. Guan, K.L. and Dixon, J.E. (1991) Eukaryotic protein expression in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192, 262-267.
6. Haystead, T.A.J., Haystead, C.M.M., Hu, C., Lin, T.A. and Lawrence, J.C. Jr. (1994) Phosphorylation of PHAS-I by mitogen-activated protein (MAP) kinase. J. Biol. Chem. 269, 23185-23191.
7. Hochuli, E. (1990) Purification of recombinant proteins with metal chelate adsorbant. In: Setlow, J.K. (Ed.), Genetic Engineering, Principle and Methods, Vol. 12. Plenum Press, New York, NY, pp 87-98.
8. Hu, C., Pang, S., Kong, X., Velleca, M., and Lawrence, J.C. Jr. (1994) Molecular cloning and tissue distribution of PHAS-I, an intracellular target for insulin and growth factor. Proc. Natl. Acad. Sci. USA 91, 3730-3734.
9. Kyriakis, J.M., Banerjee, P., Nikolakaki, E., Dai, T., Rubie, E.A., Ahmad, M.F., A.J. and Woodgett, J.R. (1994) The stress-activated protein kinase subfamily of c-Jun kinases. Nature 369, 156-160.
10. Lin, A., Minden, A., Martinetto, H., Claret, F.-X., Lange-Carter, C., Mercurio, F., Johnson, G.L. and Karin, M. (1995) Identification of a dual specificity kinase thatactivates the Jun kinase and p38-Mpk2. Sceince 268, 286-290.
11. Means, A.R., Bafchi, I.C., Van Berkum, M.F.A. and Rassmussen, C.D. (1991) Calmodulin. In: McCormack, J.G. and Cobbold, J.H. (Eds.), Cellular Calcium, A Practical Approach. IRL Press, Oxford, pp. 205-245.
12. Nilsson, B., Abrahmsen, L. and Uhlen, M. (1985) Immobilization and purification of enzymes with staphylococal protein A. gene fusion vectors. EMBO J. 4, 1075-1080.
13. Pause, A., Belsham, G.J., Gingras, A.-C., Donze, O., Lin, T.-A., Lawrence, J.C. Jr. and Sonenberg, N. (1994) Insulin-dependent stimulation of protein synthesis by phosphorylation of regulator of 5′-cap function. Nature 371, 762-767.
14. Robbins, D.J., Zhen, E., Owaki, H., Vanderbilt, C.A., Ebert, D., Geppert, T.D. and Cobb, M.H. (1993) Regulation and properties of extracellular signal-regulated protein kinase 1 and 2 in vitro. J. Biol. Chem. 268, 5097-5106.
15. Sassenfeld, H.M. and Brewer, S.J. (1984) A polypeptide fusion designed for the purification of recombinant proteins. BioTechnology 2, 76-81.
16. Smith, D.B. and Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
17. Stofko-Hahn, R.E., Carr, D.W., and Scott, J.D. (1992) A single step purification for recombinant proteins. FEBS Lett. 302, 274-302.
18. Studier, F.W. and Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
19. Takio, K., Blumenthal, D.K., Walsh, K.A., Titani, K. and Krebs, K.G. Amino acid sequence of rabbit skeletal muscle myosin light chain kinase. Biochemistry 25 (1986) 8049-8057.
20. Zheng, C.F. and Weiner, H. (1993) Attempts to increase the expression of rat liver mitochondrial aldehyde dehydrogenase in E. coli by altering the mRNA. Adv. Exp. Med. Biol. 284, 73-79.