From tubes to drops: Droplet-based microfluidics for ultrahigh-throughput biology

Journal of Physics D: Applied Physics

Volumn 46, Issue 11, 2013, Pages

  Tran, T M ^a,b   Lan, F ^a,b   Thompson, C S ^a,b   Abate, A R ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b San Francisco   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BASIC ELEMENTS; BIOLOGICAL ANALYSIS; DROPLET MANIPULATION; DROPLET-BASED MICROFLUIDICS; ITS APPLICATIONS; MICROFLUIDIC COMPONENTS;

DROPS; THROUGHPUT;

MICROFLUIDICS;

EID: 84874488132     PISSN: 00223727     EISSN: 13616463     Source Type: Journal    
DOI: 10.1088/0022-3727/46/11/114004     Document Type: Article

References (205)

1. Wang W, Walker J R, Wang X, Tremblay M S, Lee J W, Wu X and Schultz P G 2009 Proc. Natl Acad. Sci. USA 106 1427-32
2. Burbaum J J 1998 Drug Discovery Today 3 313-22
3. Major J 1998 J. Biomol. Screen 3 13-7
4. Aharoni A, Griffiths A D and Tawfik D S 2005 Curr. Opin. Chem. Biol. 9 210-6
5. Dalby P A 2003 Curr. Opin Struct. Biol. 13 500-5
6. Wittrup K D 2001 Curr. Opin. Biotechnol. 12 395-9
7. Liebeton K, Zonta A, Schimossek K, Nardini M, Lang D, Dijkstra B W, Reetz M T and Jaeger K-E 2000 Chem. Biol. 7 709-18
8. Daugherty P S, Iverson B L and Georgiou G 2000 J. Immunol. Methods 243 211-27
9. Aebersold R and Mann M 2003 Nature 422 198-207
10. Boder E T and Wittrup K D 1997 Nature Biotechnol. 15 553-7
11. Fernandez-Gacio A, Uguen M and Fastrez J 2003 Trends Biotechnol. 21 408-14
12. Forrer P, Jung S and Plückthun A 1999 Curr. Opin Struct. Biol. 9 514-20
13. Amstutz P, Forrer P, Zahnd C and Plückthun A 2001 Curr. Opin. Biotechnol. 12 400-5
14. Glieder A, Farinas E T and Arnold F H 2002 Nature Biotechnol. 20 1135-9
15. Jaeger K-E and Eggert T 2002 Curr. Opin. Biotechnol. 13 390-7
16. Turner N J 2003 Trends Biotechnol. 21 474-8
17. Williams G J, Zhang C and Thorson J S 2007 Nature Chem. Biol. 3 657-62
18. Otten L G, Schaffer M L, Villiers B R M, Stachelhaus T and Hollfelder F 2007 Biotechnol. J. 2 232-40
19. Polizzi K M, Parikh M, Spencer C U, Matsumura I, Lee J H, Realff M J and Bommarius A S 2006 Biotechnol. Prog. 22 961-7
20. Tee K L and Schwaneberg U 2006 Angew. Chem. Int. Edn Engl. 45 5380-3
21. Teh S-Y, Lin R, Hung L-H and Lee A P 2008 Lab Chip 8 198-220
22. Mukhopadhyay R 2007 Anal. Chem. 79 3248-53
23. Zeng Y, Novak R, Shuga J, Smith M T and Mathies R A 2010 Anal. Chem. 82 3183-90
24. Wu N, Zhu Y, Brown S, Oakeshott J, Peat T S, Surjadi R, Easton C, Leech P W and Sexton B A 2009 Lab Chip 9 3391-8
25. Lorenz H D 1997 J. Micromech. Microeng. 7 121-4
26. McDonald J C, Duffy D C, Anderson J R, Chiu D T, Wu H, Schueller O J A and Whitesides G M 2000 Electrophoresis 21 27-40
27. Rotem A, Abate A R, Utada A S, Steijn V V and Weitz D A 2012 Lab Chip 12 4263-8
28. Haubert K, Drier T and Beebe D 2006 Lab Chip 6 1548-9
29. Fritz J L and Owen M J 1995 J. Adhes. 54 33-45
30. Dangla R, Gallaire F and Baroud C N 2010 Lab Chip 10 2972
31. He M, Edgar J S, Jeffries G D M, Lorenz R M, Shelby J P and Chiu D T 2005 Anal. Chem. 77 1539-44
32. Lee J N, Park C and Whitesides G M 2003 Anal. Chem. 75 6544-54
33. Courtois F, Olguin L F, Whyte G, Bratton D, Huck W T S, Abell C and Hollfelder F 2008 ChemBioChem 9 439-46
34. Kumaresan P, Yang C J, Cronier S A, Blazej R G and Mathies R A 2008 Anal. Chem. 80 3522-9
35. Chen F, Zhan Y, Geng T, Lian H, Xu P and Lu C 2011 Anal. Chem. 83 8816-20
36. Studer A 1997 Science 275 823-6
37. Curran D P 1998 Angew. Chem. Int. Edn Engl. 37 1174-96
38. Lowe K C, Davey M R and Power J B 1998 Trends Biotechnol. 16 272-7
39. Schmitz C H J, Rowat A C, Köster S and Weitz D A 2009 Lab Chip 9 44
40. Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison J B, Rothberg J M, Link D R, Perrimon N and Samuels M L 2009 Proc. Natl Acad. Sci. USA 106 14195-200
41. Pan J, Stephenson A L, Kazamia E, Huck W T, Dennis J S, Smith A G and Abell C 2011 Integr. Biol. (Camb.) 3 1043-51
42. Clausell-Tormos J et al 2008 Chem. Biol. 15 427-37
43. Sadtler V M, Krafft M P and Riess J G 1996 Angew. Chem. Int. Edn Engl. 35 1976-8
44. Guo M T, Rotem A, Heyman J A and Weitz D A 2012 Lab Chip 12 2146
45. Bai Y, He X, Liu D, Patil S N, Bratton D, Huebner A, Hollfelder F, Abell C and Huck W T S 2010 Lab Chip 10 1281
46. Baret J-C 2012 Lab Chip 12 422
47. Holtze C et al 2008 Lab Chip 8 1632
48. Roach L S, Song H and Ismagilov R F 2005 Anal. Chem 77 785-96
49. Sadtler V M, Jeanneaux F, Pierre Krafft M, Rábai J and Riess J G 1998 New J. Chem. 22 609-13
50. Liau A, Karnik R, Majumdar A and Cate J H D 2005 Anal. Chem. 77 7618-25
51. Courtois F, Olguin L F, Whyte G, Theberge A B, Huck W T S, Hollfelder F and Abell C 2009 Anal. Chem. 81 3008-16
52. Kaltenbach M, Devenish S R A and Hollfelder F 2012 Lab Chip 12 4185
53. Paegel B M and Joyce G F 2010 Chem. Biol. 17 717-24
54. Christopher G F and Anna S L 2007 J. Phys. D. Appl. Phys. 40 R319-R336
55. Vladisavljeviæ G, Kobayashi I and Nakajima M 2012 Microfluid. Nanofluid. 13 151-78
56. Seemann R, Brinkmann M, Pfohl T and Herminghaus S 2012 Rep. Prog. Phys. 75 016601
57. Utada A S, Fernandez-Nieves A, Stone H A and Weitz D A 2007 Phys. Rev. Lett. 99 094502
58. Utada A S, Fernandez-Nieves A, Gordillo J M and Weitz D A 2008 Phys. Rev. Lett. 100 014502
59. Cramer C, Fischer P and Windhab E J 2004 Chem. Eng. Sci. 59 3045-58
60. Guillot P, Colin A and Ajdari A 2008 Phys. Rev. E 78 016307
61. Thorsen T, Roberts R W, Arnold F H and Quake S R 2001 Phys. Rev. Lett. 86 4163-6
62. Garstecki P, Fuerstman M J, Stone H A and Whitesides G M 2006 Lab Chip 6 437
63. Anna S L, Bontoux N and Stone H A 2003 Appl. Phys. Lett. 82 364
64. Abate A R, Poitzsch A, Hwang Y, Lee J, Czerwinska J and Weitz D A 2009 Phys. Rev. E 80 026310
65. De Menech M, Garstecki P, Jousse F and Stone H A 2008 J. Fluid Mech. 595 141-61
66. Nie Z, Seo M, Xu S, Lewis P, Mok M, Kumacheva E, Whitesides G, Garstecki P and Stone H 2008 Microfluid. Nanofluid. 5 585-94
67. Link D R, Anna S L, Weitz D A and Stone H A 2004 Phys. Rev. Lett. 92 054503
68. Abate A R and Weitz D A 2011 Lab Chip 11 1911
69. Abate A R and Weitz D A 2011 Lab Chip 11 1713
70. Nisisako T, Torii T, Takahashi T and Takizawa Y 2006 Adv. Mater. 18 1152-6
71. Nisisako T and Torii T 2008 Lab Chip 8 287
72. Zeng Y, Novak R, Shuga J, Smith M T and Mathies R A 2010 Anal. Chem. 82 3183-90
73. Abate A R, Romanowsky M B, Agresti J J and Weitz D A 2009 Appl. Phys. Lett. 94 023503
74. Priest C, Herminghaus S and Seemann R 2006 Appl. Phys. Lett. 88 024106
75. Chokkalingam V, Herminghaus S and Seemann R 2008 Appl. Phys. Lett. 93 254101
76. Köster S et al 2008 Lab Chip 8 1110
77. Huebner A, Srisa-Art M, Holt D, Abell C, Hollfelder F, deMello A J and Edel J B 2007 Chem. Commun. 1218
78. Kemna E W M, Schoeman R M, Wolbers F, Vermes I, Weitz D A and Berg A van den 2012 Lab Chip 12 2881-7
79. Abate A R, Chen C-H, Agresti J J and Weitz D A 2009 Lab Chip 9 2628
80. Chabert M and Viovy J-L 2008 Proc. Natl Acad. Sci. 105 3191-6
81. Um E, Lee S-G and Park J-K 2010 Appl. Phys. Lett. 97 153703
82. Tawfik D S and Griffiths A D 1998 Nature Biotechnol. 16 652-6
83. Dressman D, Yan H, Traverso G, Kinzler K W and Vogelstein B 2003 Proc. Natl Acad. Sci. 100 8817-22
84. Nakano M, Komatsu J, Matsuura S, Takashima K, Katsura S and Mizuno A 2003 J. Biotechnol. 102 117-24
85. Bremond N, Thiam A R and Bibette J 2008 Phys. Rev. Lett. 100 024501
86. Mazutis L, Baret J-C and Griffiths A D 2009 Lab Chip 9 2665
87. Mazutis L and Griffiths A D 2012 Lab Chip 12 1800
88. Fidalgo L M, Abell C and Huck W T S 2007 Lab Chip 7 984
89. Chabert M, Dorfman K D and Viovy J-L 2005 Electrophoresis 26 3706-15
90. Baroud C N, Robert de Saint Vincent M and Delville J-P 2007 Lab Chip 7 1029
91. Link D R, Grasland-Mongrain E, Duri A, Sarrazin F, Cheng Z, Cristobal G, Marquez M and Weitz D A 2006 Angew. Chem. Int. Edn Engl. 45 2556-60
92. Tewhey R et al 2009 Nature Biotechnol. 27 1025-31
93. Thiam A R, Bremond N and Bibette J 2009 Phys. Rev. Lett. 102 188304
94. Ahn K, Agresti J, Chong H, Marquez M and Weitz D A 2006 Appl. Phys. Lett. 88 264105
95. Mary P, Chen A, Chen I, Abate A R and Weitz D A 2011 Lab Chip 11 2066
96. Fallah-Araghi A, Baret J-C, Ryckelynck M and Griffiths A D 2012 Lab Chip 12 882
97. Tan W-H and Takeuchi S 2006 Lab Chip 6 757
98. Zagnoni M and Cooper J M 2009 Lab Chip 9 2652
99. Zagnoni M, Le Lain G and Cooper J M 2010 Langmuir 26 14443-9
100. Abate A R, Hung T, Mary P, Agresti J J and Weitz D A 2010 Proc. Natl Acad. Sci. USA 107 19163-6
101. Handique K and Burns M A 2001 J. Micromech. Microeng. 11 548-54
102. Tice J D, Song H, Lyon A D and Ismagilov R F 2003 Langmuir 19 9127-33
103. Bringer M R, Gerdts C J, Song H, Tice J D and Ismagilov R F 2004 Phil. Trans. R. Soc. Lond. A 362 1087-104
104. Jiang L, Zeng Y, Zhou H, Qu J Y and Yao S 2012 Biomicrofluidics 6 012810
105. Song H, Tice J D and Ismagilov R F 2003 Angew Chem. Int. Edn Engl. 115 792-6
106. Liau A, Karnik R, Majumdar A and Cate J H D 2005 Anal. Chem. 77 7618-25
107. Kiss M M, Ortoleva-Donnelly L, Beer N R, Warner J, Bailey C G, Colston B W, Rothberg J M, Link D R and Leamon J H 2008 Anal. Chem. 80 8975-81
108. Theberge A B, Mayot E, El Harrak A, Kleinschmidt F, Huck W T S and Griffiths A D 2012 Lab Chip 12 1320
109. Leng X, Zhang W, Wang C, Cui L and Yang C J 2010 Lab Chip 10 2841
110. Novak R, Zeng Y, Shuga J, Venugopalan G, Fletcher D A, Smith M T and Mathies R A 2011 Angew. Chem. Int. Edn Engl. 123 410-5
111. Sepp A, Tawfik D S and Griffiths A D 2002 FEBS Lett. 532 455-8
112. Levy M, Griswold K E and Ellington A D 2005 RNA 11 1555-62
113. Diehl F, Li M, He Y, Kinzler K W, Vogelstein B and Dressman D 2006 Nature Methods 3 551-9
114. Margulies M et al 2005 Nature 437 376-80
115. Fidalgo L M, Whyte G, Bratton D, Kaminski C F, Abell C and Huck W T S 2008 Angew. Chem. Int. Edn Engl. 47 2042-5
116. Pan X, Zeng S, Zhang Q, Lin B and Qin J 2011 Electrophoresis 32 3399-405
117. Adamson D N, Mustafi D, Zhang J X J, Zheng B and Ismagilov R F 2006 Lab Chip 6 1178
118. Liu W, Kim H J, Lucchetta E M, Du W and Ismagilov R F 2009 Lab Chip 9 2153
119. Clausell-Tormos J, Griffiths A D and Merten C A 2010 Lab Chip 10 1302
120. Sgro A E, Allen P B and Chiu D T 2007 Anal. Chem. 79 4845-51
121. Stan C A, Schneider G F, Shevkoplyas S S, Hashimoto M, Ibanescu M, Wiley B J and Whitesides G M 2009 Lab Chip 9 2293
122. Song H and Ismagilov R F 2003 J. Am. Chem. Soc. 125 14613-9
123. Chanasakulniyom M, Martino C, Paterson D, Horsfall L, Rosser S and Cooper J M 2012 Analyst 137 2939
124. Frenz L, Blank K, Brouzes E and Griffiths A D 2009 Lab Chip 9 1344
125. Hofmann T W, Hänselmann S, Janiesch J-W, Rademacher A and Böhm C H J 2012 Lab Chip 12 916
126. Edd J F, Humphry K J, Irimia D, Weitz D A and Toner M 2009 Lab Chip 9 1859
127. Hatch A C, Fisher J S, Tovar A R, Hsieh A T, Lin R, Pentoney S L, Yang D L and Lee A P 2011 Lab Chip 11 3838
128. Huebner A, Bratton D, Whyte G, Yang M, deMello A J, Abell C and Hollfelder F 2009 Lab Chip 9 692
129. Laval P, Lisai N, Salmon J-B and Joanicot M 2007 Lab Chip 7 829
130. Um E, Rha E, Choi S-L, Lee S-G and Park J-K 2012 Lab Chip 12 1594
131. Boukellal H, Selimoviæ Š, Jia Y, Cristobal G and Fraden S 2009 Lab Chip 9 331
132. Lau B T C, Baitz C A, Dong X P and Hansen C L 2007 J. Am. Chem. Soc. 129 454-5
133. Joensson H N, Samuels M L, Brouzes E R, Medkova M, Uhlén M, Link D R and Andersson-Svahn H 2009 Angew. Chem. Int. Edn Engl. 121 2556-9
134. Pekin D et al 2011 Lab Chip 11 2156-66
135. Mazutis L, Baret J-C, Treacy P, Skhiri Y, Araghi A F, Ryckelynck M, Taly V and Griffiths A D 2009 Lab Chip 9 2902
136. Hatakeyama T, Chen D L and Ismagilov R F 2006 J. Am. Chem. Soc. 128 2518-9
137. Zheng B and Ismagilov R F 2005 Angew. Chem. Int. Edn Engl. 44 2520-3
138. Mazutis L and Griffiths A D 2009 Appl. Phys. Lett. 95 204103
139. Zhang K, Liang Q, Ma S, Mu X, Hu P, Wang Y and Luo G 2009 Lab Chip 9 2992
140. Al-Hetlani E, Hatt O J, Vojtiìsěk M, Tarn M D and Pamme N 2010 14th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (Groningen, The Netherlands,) pp 167-75
141. Abate A R, Agresti J J and Weitz D A 2010 Appl. Phys. Lett. 96 203509
142. Franke T, Abate A R, Weitz D A and Wixforth A 2009 Lab Chip 9 2625
143. Ahn K, Kerbage C, Hunt T P, Westervelt R M, Link D R and Weitz D A 2006 Appl. Phys. Lett. 88 024104
144. Baret J-C et al 2009 Lab Chip 9 1850
145. Agresti J J, Antipov E, Abate A R, Ahn K, Rowat A C, Baret J-C, Marquez M, Klibanov A M, Griffiths A D and Weitz D A 2010 Proc. Natl Acad. Sci. 107 4004-9
146. Bernath K, Hai M, Mastrobattista E, Griffiths A D, Magdassi S and Tawfik D S 2004 Anal. Biochem. 325 151-7
147. Mastrobattista E, Taly V, Chanudet E, Treacy P, Kelly B T and Griffiths A D 2005 Chem. Biol. 12 1291-300
148. Miller O J et al 2012 Proc. Natl Acad. Sci. USA 109 378-83
149. Zheng B, Roach L S and Ismagilov R F 2003 J. Am. Chem. Soc. 125 11170-1
150. Kintses B, van Vliet L D, Devenish S R A and Hollfelder F 2010 Curr. Opin. Chem. Biol. 14 548-55
151. Pompano R R, Liu W, Du W and Ismagilov R F 2011 Annu. Rev. Anal. Chem. 4 59-81
152. Chen D L and Ismagilov R F 2006 Curr. Opin. Chem. Biol. 10 226-31
153. Schonbrun E, Abate A R, Steinvurzel P E, Weitz D A and Crozier K B 2010 Lab Chip 10 852
154. He M, Edgar J S, Jeffries G D M, Lorenz R M, Shelby J P and Chiu D T 2005 Anal. Chem. 77 1539-44
155. Srisa-Art M, deMello A J and Edel J B 2007 Anal. Chem. 79 6682-9
156. Zheng B, Tice J D and Ismagilov R F 2004 Anal. Chem. 76 4977-82
157. Baret J-C, Beck Y, Billas-Massobrio I, Moras D and Griffiths A D 2010 Chem. Biol. 17 528-36
158. Melkko S, Zhang Y, Dumelin C E, Scheuermann J and Neri D 2007 Angew. Chem. Int. Edn Engl. 46 4671-4
159. Portney N G, Wu Y, Quezada L K, Lonardi S and Ozkan M 2008 Langmuir 24 1613-6
160. Lorenceau E, Utada A S, Link D R, Cristobal G, Joanicot M and Weitz D A 2005 Langmuir 21 9183-6
161. Shah R K, Kim J-W, Agresti J J, Weitz D A and Chu L-Y 2008 Soft Matter 4 2303
162. Ji X-H, Zhang N-G, Cheng W, Guo F, Liu W, Guo S-S, He Z-K and Zhao X-Z 2011 J. Mater Chem. 21 13380
163. Theilacker N, Roller E E, Barbee K D, Franzreb M and Huang X 2011 J. R. Soc. Interface 8 1104-13
164. Zhang P, He Y, Ruan Z, Chen F F and Yang J 2012 J. Colloid Interface Sci. 385 8-14
165. Miller M B and Tang Y-W 2009 Clin. Microbiol. Rev. 22 611-33
166. Niu X Z, Zhang B, Marszalek R T, Ces O, Edel J B, Klug D R and deMello A J 2009 Chem. Commun. 6159-61
167. Ji J, Nie L, Qiao L, Li Y, Guo L, Liu B, Yang P and Girault H H 2012 Lab Chip 12 2625
168. Liu S, Gu Y, Le Roux R B, Matthews S M, Bratton D, Yunus K, Fisher A C and Huck W T S 2008 Lab Chip 8 1937
169. Luo C, Yang X, Fu Q, Sun M, Ouyang Q, Chen Y and Ji H 2006 Electrophoresis 27 1977-83
170. Cristobal G, Arbouet L, Sarrazin F, Talaga D, Bruneel J-L, Joanicot M and Servant L 2006 Lab Chip 6 1140
171. Strehle K R, Cialla D, Rösch P, Henkel T, Köhler M and Popp J 2007 Anal. Chem. 79 1542-7
172. Wang G, Lim C, Chen L, Chon H, Choo J, Hong J and deMello A J 2009 Anal. Bioanal. Chem. 394 1827-32
173. Ackermann K R, Henkel T and Popp J 2007 ChemPhysChem 8 2665-70
174. März A, Ackermann K R, Malsch D, Bocklitz T, Henkel T and Popp J 2009 J. Biophoton. 2 232-42
175. Cecchini M P, Hong J, Lim C, Choo J, Albrecht T, deMello A J and Edel J B 2011 Anal. Chem. 83 3076-81
176. Choi J-W, Kang D-K, Park H, deMello A J and Chang S-I 2012 Anal. Chem. 84 3849-54
177. Granieri L, Baret J C, Griffiths A D and Merten C A 2010 Chem. Biol. 17 229-35
178. Nguyen N-T, Lassemono S and Chollet F A 2006 Sensors Actuators B 117 431-6
179. Srisa-Art M, Dyson E C, deMello A J and Edel J B 2008 Anal. Chem. 80 7063-7
180. Srisa-Art M, Kang D-K, Hong J, Park H, Leatherbarrow R J, Edel J B, Chang S-I and deMello A J 2009 ChemBioChem 10 1605-11
181. Srisa-Art M, deMello A and Edel J 2008 Phys. Rev. Lett. 101 014502
182. Srisa-Art M, deMello A J and Edel J B 2009 Chem. Commun. 6548
183. Niu X, Gielen F, Edel J B and deMello A J 2011 Nature Chem. 3 437-42
184. Srisa-Art M, Bonzani I C, Williams A, Stevens M M, deMello A J and Edel J B 2009 Analyst 134 2239
185. Wang J, Jiang P, Han Z, Qiu L, Wang C, Zheng B and Xia J 2012 Langmuir 28 7962-6
186. Casadevall i Solvas X, Srisa-Art M, deMello A J and Edel J B 2010 Anal. Chem. 82 3950-6
187. Cherry J R and Fidantsef A L 2003 Curr. Opin. Biotechnol. 14 438-43
188. Dove A 1999 Nature Biotechnol. 17 859-63
189. Gong Z, Zhao H, Zhang T, Nie F, Pathak P, Cui K, Wang Z, Wong S and Que L 2011 Biomed. Microdevices 13 215-9
190. Zhan Y, Wang J, Bao N and Lu C 2009 Anal. Chem. 81 2027-31
191. Rotman B 1961 Proc. Natl Acad. Sci. USA 47 1981-91
192. Hindson B J et al 2011 Anal. Chem. 83 8604-10
193. Zhang H, Jenkins G, Zou Y, Zhu Z and Yang C J 2012 Anal. Chem. 84 3599-606
194. Boedicker J Q, Li L, Kline T R and Ismagilov R F 2008 Lab Chip 8 1265-72
195. Chao W C, Collins J, Wang S W, Li G P, Bachman M and Lee A P 2004 Conf. Proc. IEEE Eng. Med. Biol. Soc. 4 2623-6
196. Maeki M, Yoshizuka S, Yamaguchi H, Kawamoto M, Yamashita K, Nakamura H, Miyazaki M and Maeda H 2012 Anal. Sci. 28 65
197. Chayen N E and Saridakis E 2008 Nature Methods 5 147-53
198. Zhang W Y, Zhang W, Liu Z, Li C, Zhu Z and Yang C J 2011 Anal. Chem. 84 350-5
199. Konry T, Dominguez-Villar M, Baecher-Allan C, Hafler D A and Yarmush M L 2011 Biosensors Bioelectron. 26 2707-10
200. Zhong Q, Bhattacharya S, Kotsopoulos S, Olson J, Taly V, Griffiths A D, Link D R and Larson J W 2011 Lab Chip 11 2167-74
201. Mazutis L et al 2009 Anal. Chem. 81 4813-21
202. Whale A S, Huggett J F, Cowen S, Speirs V, Shaw J, Ellison S, Foy C A and Scott D J 2012 Nucl. Acids Res. 40 e82
203. Heyries K A, Tropini C, Vaninsberghe M, Doolin C, Petriv O I, Singhal A, Leung K, Hughesman C B and Hansen C L 2011 Nature Methods 8 649-51
204. Hussein S M et al 2011 Nature 471 58-62
205. Zhu Z, Jenkins G, Zhang W, Zhang M, Guan Z and Yang C J 2012 Anal. Bioanal. Chem. 403 2127-43