Microfluidic droplets: New integrated workflows for biological experiments

Current Opinion in Chemical Biology

Volumn 14, Issue 5, 2010, Pages 548-555

  Kintses, Balint ^a   van Vliet, Liisa D ^a   Devenish, Sean R A ^a   Hollfelder, Florian ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; CYTOTOXIN; DNA; ENZYME; FLUORESCENT DYE; OIL; PROTEIN; QUANTUM DOT; WATER;

BIOLOGY; CELL ENCAPSULATION; CELL GROWTH; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME METABOLISM; EXPERIMENTATION; GENE AMPLIFICATION; HIGH THROUGHPUT SCREENING; HUMAN; MICROFLUIDIC DROPLET; MICROFLUIDICS; MOLECULAR EVOLUTION; NANOENCAPSULATION; NONHUMAN; ORGANISM; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; REVIEW;

ANIMALS; BIOLOGY; DIAGNOSIS; DIRECTED MOLECULAR EVOLUTION; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 77957755250     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2010.08.013     Document Type: Review

References (61)

1. Margulies M., Egholm M., Altman W.E., Attiya S., Bader J.S., Bemben L.A., Berka J., Braverman M.S., Chen Y., Chen Z., et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature 2005, 437:376-380.
2. Schaerli Y., Hollfelder F. The potential of microfluidic water-in-oil droplets in experimental biology. Mol Biosyst 2009, 5:1392-1404.
3. Kelly B.T., Baret J.C., Taly V., Griffiths A.D. Miniaturizing chemistry and biology in microdroplets. Chem Commun 2007, 1773-1788.
4. Agresti J.J., Antipov E., Abate A.R., Ahn K., Rowat A.C., Baret J.C., Marquez M., Klibanov A.M., Griffiths A.D., Weitz D.A. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc Natl Acad Sci U S A 2010, 107:4004-4009.
5. Huebner A., Sharma S., Srisa-Art M., Hollfelder F., Edel J.B., deMello A.J. Microdroplets: a sea of applications?. Lab Chip 2008, 8:1244-1254.
6. Theberge A.B., Courtois F., Schaerli Y., Fischlechner M., Abell C., Hollfelder F., Huck W.T.S. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed 2010, 49:5846-5868.
7. Link D.R., Grasland-Mongrain E., Duri A., Sarrazin F., Cheng Z., Cristobal G., Marquez M., Weitz D.A. Electric control of droplets in microfluidic devices. Angew Chem Int Ed 2006, 45:2556-2560.
8. Priest C., Herminghaus S., Seemann R. Controlled electrocoalescence in microfluidics: targeting a single lamella. Appl Phys Lett 2006, 89:134101.
9. Zagnoni M., Baroud C.N., Cooper J.M. Electrically initiated upstream coalescence cascade of droplets in a microfluidic flow. Phys Rev E Stat Nonlin Soft Matter Phys 2009, 80:046303.
10. Niu X., Gielen F., deMello A.J., Edel J.B. Electro-coalescence of digitally controlled droplets. Anal Chem 2009, 81:7321-7325.
11. Baroud C.N., de Saint Vincent M.R., Delville J.P. An optical toolbox for total control of droplet microfluidics. Lab Chip 2007, 7:1029-1033.
12. Verneuil E., Cordero M., Gallaire F., Baroud C.N. Laser-induced force on a microfluidic drop: origin and magnitude. Langmuir 2009, 25:5127-5134.
13. Niu X., Gulati S., Edel J.B., deMello A.J. Pillar-induced droplet merging in microfluidic circuits. Lab Chip 2008, 8:1837-1841.
14. Mazutis L., Baret J.C., Griffiths A.D. A fast and efficient microfluidic system for highly selective one-to-one droplet fusion. Lab Chip 2009, 9:2665-2672.
15. Frenz L., Blank K., Brouzes E., Griffiths A.D. Reliable microfluidic on-chip incubation of droplets in delay-lines. Lab Chip 2009, 9:1344-1348.
16. Courtois F., Olguin L.F., Whyte G., Theberge A.B., Huck W.T.S., Hollfelder F., Abell C. Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Anal Chem 2009, 81:3008-3016.
17. Huebner A., Bratton D., Whyte G., Yang M., deMello A.J., Abell C., Hollfelder F. Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab Chip 2009, 9:692-698.
18. Köster S., Angile F.E., Duan H., Agresti J.J., Wintner A., Schmitz C., Rowat A.C., Merten C.A., Pisignano D., Griffiths A.D., et al. Drop-based microfluidic devices for encapsulation of single cells. Lab Chip 2008, 8:1110-1115.
19. Shim J.U., Olguin L.F., Whyte G., Scott D., Babtie A., Abell C., Huck W.T.S., Hollfelder F. Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc 2009, 131:15251-15256.
20. Schmitz C.H.J., Rowat A.C., Köster S., Weitz D.A. Dropspots: a picoliter array in a microfluidic device. Lab Chip 2009, 9:44-49.
21. Clausell-Tormos J., Lieber D., Baret J.C., El-Harrak A., Miller O.J., Frenz L., Blouwolff J., Humphry K.J., Köster S., Duan H., et al. Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms. Chem Biol 2008, 15:427-437.
22. Mazutis L., Baret J.C., Treacy P., Skhiri Y., Araghi A.F., Ryckelynck M., Taly V., Griffiths A.D. Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme. Lab Chip 2009, 9:2902-2908.
23. Niu X.Z., Zhang B., Marszalek R.T., Ces O., Edel J.B., Klug D.R., deMello A.J. Droplet-based compartmentalization of chemically separated components in two-dimensional separations. Chem Commun 2009, 6159-6161.
24. Song H., Chen D.L., Ismagilov R.F. Reactions in droplets in microfluidic channels. Angew Chem Int Ed 2006, 45:7336-7356.
25. Joensson H.N., Samuels M.L., Brouzes E.R., Medkova M., Uhlen M., Link D.R., Andersson-Svahn H. Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets. Angew Chem Int Ed 2009, 48:2518-25121.
26. Srisa-Art M., deMello A.J., Edel J.B. High-throughput confinement and detection of single DNA molecules in aqueous microdroplets. Chem Commun 2009, 6548-6550.
27. Schaerli Y., Wootton R.C., Robinson T., Stein V., Dunsby C., Neil M.A., French P.M., deMello A.J., Abell C., Hollfelder F. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal Chem 2009, 81:302-306.
28. Huebner A., Olguin L.F., Bratton D., Whyte G., Huck W.T.S., deMello A.J., Edel J.B., Abell C., Hollfelder F. Development of quantitative cell-based enzyme assays in microdroplets. Anal Chem 2008, 80:3890-3896.
29. Robinson T., Schaerli Y., Wootton R.C., Hollfelder F., Dunsby C., Baldwin G.S., Neil M.A., French P.M., deMello A.J. Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip 2009, 9:3437-3441.
30. Baroud C.N., Delville J.P., Gallaire F., Wunenburger R. Thermocapillary valve for droplet production and sorting. Phys Rev E Stat Nonlin Soft Matter Phys 2007, 75:046302.
31. Franke T., Abate A.R., Weitz D.A., Wixforth A. Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab Chip 2009, 9:2625-2627.
32. Baret J.C., Miller O.J., Taly V., Ryckelynck M., El-Harrak A., Frenz L., Rick C., Samuels M.L., Hutchison J.B., Agresti J.J., et al. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 2009, 9:1850-1858.
33. Granieri L., Baret J.C., Griffiths A.D., Merten C.A. High-throughput screening of enzymes by retroviral display using droplet-based microfluidics. Chem Biol 2010, 17:229-235.
34. Roach L.S., Song H., Ismagilov R.F. Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. Anal Chem 2005, 77:785-796.
35. Holtze C., Rowat A.C., Agresti J.J., Hutchison J.B., Angilè F.E., Schmitz C.H.J., Köster S., Duan H., Humphry K.J., Scanga R.A., et al. Biocompatible surfactants for water-in-fluorocarbon emulsions. Lab Chip 2008, 8:1632.
36. Chabert M., Viovy J. Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells. Proc Natl Acad Sci U S A 2008, 105:3191-3196.
37. Edd J.F., Carlo D.D., Humphry K.J., Köster S., Irimia D., Weitz D.A., Toner M. Controlled encapsulation of single-cells into monodisperse picolitre drops. Lab Chip 2008, 8:1262-1264.
38. Abate A.R., Chen C.H., Agresti J.J., Weitz D.A. Beating Poisson encapsulation statistics using close-packed ordering. Lab Chip 2009, 9:2628-2631.
39. Kiss M.M., Ortoleva-Donnelly L., Beer N.R., Warner J., Bailey C.G., Colston B.W., Rothberg J.M., Link D.R., Leamon J.H. High-throughput quantitative polymerase chain reaction in picoliter droplets. Anal Chem 2008, 80:8975-8981.
40. Beer N.R., Hindson B.J., Wheeler E.K., Hall S.B., Rose K.A., Kennedy I.M., Colston B.W. On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. Anal Chem 2007, 79:8471-8475.
41. Beer N.R., Wheeler E.K., Lee-Houghton L., Watkins N., Nasarabadi S., Hebert N., Leung P., Arnold D.W., Bailey C.G., Colston B.W. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets. Anal Chem 2008, 80:1854-1858.
42. Tewhey R., Warner J.B., Nakano M., Libby B., Medkova M., David P.H., Kotsopoulos S.K., Samuels M.L., Hutchison J.B., Larson J.W., et al. Microdroplet-based PCR enrichment for large-scale targeted sequencing. Nat Biotechnol 2009, 27:1025-1031.
43. Zhang Y., Bailey V., Puleo C.M., Easwaran H., Griffiths E., Herman J.G., Baylin S.B., Wang T. DNA methylation analysis on a droplet-in-oil PCR array. Lab Chip 2009, 9:1059-1064.
44. Zeng Y., Novak R., Shuga J., Smith M.T., Mathies R.A. High-performance single cell genetic analysis using microfluidic emulsion generator arrays. Anal Chem 2010, 82:3183-3190.
45. Mazutis L., Araghi A.F., Miller O.J., Baret J.C., Frenz L., Janoshazi A., Taly V., Miller B.J., Hutchison J.B., Link D., et al. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. Anal Chem 2009, 81:4813-4821.
46. Courtois F., Olguin L.F., Whyte G., Bratton D., Huck W.T.S., Abell C., Hollfelder F. An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. ChemBioChem 2008, 9:439-446.
47. Huebner A., Srisa-Art M., Holt D., Abell C., Hollfelder F., deMello A.J., Edel J.B. Quantitative detection of protein expression in single cells using droplet microfluidics. Chem Commun 2007, 1218-1220.
48. Hufnagel H., Huebner A., Gülch C., Güse K., Abell C., Hollfelder F. An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab Chip 2009, 9:1576-1582.
49. Brouzes E., Medkova M., Savenelli N., Marran D., Twardowski M., Hutchison J.B., Rothberg J.M., Link D.R., Perrimon N., Samuels M.L. Droplet microfluidic technology for single-cell high-throughput screening. Proc Natl Acad Sci U S A 2009, 106:14195-14200.
50. Shi W., Qin J., Ye N., Lin B. Droplet-based microfluidic system for individual Caenorhabditis elegans assay. Lab Chip 2008, 8:1432-1435.
51. Tawfik D.S., Griffiths A.D. Man-made cell-like compartments for molecular evolution. Nat Biotechnol 1998, 16:652-656.
52. Cohen H.M., Tawfik D.S., Griffiths A.D. Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. Prot Eng Des Sel 2004, 17:3-11.
53. d'Abbadie M., Hofreiter M., Vaisman A., Loakes D., Gasparutto D., Cadet J., Woodgate R., Pääbo S., Holliger P. Molecular breeding of polymerases for amplification of ancient DNA. Nat Biotechnol 2007, 25:939-943.
54. Williams R., Peisajovich S.G., Miller O.J., Magdassi S., Tawfik D.S., Griffiths A.D. Amplification of complex gene libraries by emulsion PCR. Nat Methods 2006, 3:545-550.
55. Clausell-Tormos J., Griffiths A.D., Merten C.A. An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries. Lab Chip 2010, 10:1302-1307.
56. Fournier-Bidoz S., Jennings T.L., Klostranec J.M., Fung W., Rhee A., Li D., Chan W.C.W. Facile and rapid one-step mass preparation of quantum-dot barcodes. Angew Chem Int Ed 2008, 120:5659-5663.
57. Clark M.A., Acharya R.A., Arico-Muendel C.C., Belyanskaya S.L., Benjamin D.R., Carlson N.R., Centrella P.A., Chiu C.H., Creaser S.P., Cuozzo J.W., et al. Design, synthesis and selection of DNA-encoded small-molecule libraries. Nat Chem Biol 2009, 5:647-654.
58. Fidalgo L.M., Whyte G., Ruotolo B.T., Benesch J.L.P., Stengel F., Abell C., Robinson C.V., Huck W.T.S. Coupling microdroplet microreactors with mass spectrometry: reading the contents of single droplets online. Angew Chem Int Ed 2009, 48:3665-3668.
59. Link D.R., Anna S.L., Weitz D.A., Stone H.A. Geometrically mediated breakup of drops in microfluidic devices. Phys Rev Lett 2004, 92:054503.
60. Utada A.S., Lorenceau E., Link D.R., Kaplan P.D., Stone H.A., Weitz D.A. Monodisperse double emulsions generated from a microcapillary device. Science 2005, 308:537-541.
61. Dittrich P.S., Jahnz M., Schwille P. A new embedded process for compartmentalized cell-free protein expression and on-line detection in microfluidic devices. ChemBioChem 2005, 6:811-814.