Control of concentration and volume gradients in microfluidic droplet arrays for protein crystallization screening

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

Volumn 26 IV, Issue , 2004, Pages 2623-2626

  Chao, Wen Chi ^a   Collins, John ^b   Wang, Szu Wen ^b   Li, G P ^a,b   Bachman, Mark ^a,b   Lee, Abraham P ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of California at Irvine   (United States)

Author keywords

Droplet arrays; Microfluidics; Screenings

Indexed keywords

DROPLET ARRAYS; MICROFLUIDICS; SCREENINGS; ZERO-DEAD-VOLUME VALVES;

CAMERAS; CHARGE COUPLED DEVICES; CRYSTALLIZATION; FLUORESCENCE; GRADIENT METHODS; MICROPROCESSOR CHIPS; OPTIMIZATION; PHASE DIAGRAMS; POLYMERIZATION; PROTEINS;

SCREENING;

EID: 11144294820     PISSN: 05891019     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (10)

1. Yung-Chieh Ten, Vittorio Cristini, and Abraham P. Lee, "Design of Microfluidic Channel Geometries for Droplet Emulsion ", submitted to Lab on a Chip 2004
2. C.L. Hansen, E. Skordalakes, J.M. Berger, and S.R. Quake, "A Robust and Scalable Microfluidic Metering Method that Allows Protein Crystal Growth by Free Interface Diffusion", Proc. Nat'l Acad. Sci., vol. 99, 16531-16536, Dec. 2002.
3. Bo Zheng, L. Spencer Roach, and Rustem F. Ismagilov, "Screening of Protein Crystallization Conditions on a Microfluidic Chip Using Nanoliter-Size Droplets", J. American Chemical Society 2003, 125, 11170-11171
4. Todd Thorsen, R.W. Roberts, F.H. Arnold, and Stephen R. Quake, "Dynamic Pattern Formation in a Vesicle-Generating Microfluidic Device", PhysRevLett., vol. 86, no. 18, 4163-4166, Apr. 2001.
5. Joshua D. Tice, Adam D. Lyon, Rustem F. Ismagilov, "Effects of viscosity on droplet formation and mixing in microfluidic channels", Analytica Chimica Acta 507, 73-77, 2004
6. Mikol V. and Giege R. (1989), "Phase diagram of a crystalline protein: Determination of the solubility of concanavalin A by a microquantitation assay", J. Crystal Growth 97, 324-332.
7. Wen-Chi Chao, John Collins, Mark Bachman, G.P. Li, and Abraham P. Lee, "Droplet Arrays in Microfluidic Channels for Combinatorial Screening Assays", Hilton Head 2004: A Solid State Sensor, Actuator and Microsystems Workshop.
8. Amir Y. Mirarefi, Charles, F. Zukoski, "Gradient diffusion and protein solubility: use of dynamic light scattering to localize crystallization conditions ", J. Crystal Growth, accepted Jan. 2004
9. Casay G., Wilson W., "Laser scattering in a hanging drop vapor diffusion apparatus for protein crystal growth in a microgravity environment", J. Cryst. Growth, 122, 95-101, 1992
10. Bergfors TM (ed)., "Protein Crystallization", International University Line, La Jolla, CA, 1999