An optimized ATP/PPi-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis

Biotechnology Journal

Volumn 2, Issue 2, 2007, Pages 232-240

  Otten, Linda G ^a   Schaffer, Michelle L ^a   Villiers, Benoit R M ^a   Stachelhaus, Torsten ^b   Hollfelder, Florian ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

ATP PPi exchange assay; Combinatorial biosynthesis; High throughput screening; Non ribosomal peptide synthetases; TycA

Indexed keywords

ADENOSINE TRIPHOSPHATE; NATURAL PRODUCT; PEPTIDE DERIVATIVE; PEPTIDE SYNTHASE; PHOSPHORUS 32; PYROPHOSPHORIC ACID DERIVATIVE; TYROCIDINE;

ACCURACY; ADENYLATION; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; QUALITY CONTROL; QUANTITATIVE ANALYSIS; SCINTILLATION COUNTING;

ADENOSINE TRIPHOSPHATE; BINDING SITES; BIOTECHNOLOGY; CATALYTIC DOMAIN; COMBINATORIAL CHEMISTRY TECHNIQUES; DIPHOSPHATES; KINETICS; MODELS, CHEMICAL; MOLECULAR STRUCTURE; PEPTIDE SYNTHASES; REPRODUCIBILITY OF RESULTS; SUBSTRATE SPECIFICITY; TYROCIDINE;

EID: 34248379555     PISSN: 18606768     EISSN: None     Source Type: Journal    
DOI: 10.1002/biot.200600220     Document Type: Article

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