Mapping and partial characterization of proteases expressed by a CHO production cell line

Biotechnology and Bioengineering

Volumn 95, Issue 5, 2006, Pages 961-971

  Sandberg, H ^a   Lutkemeyer D ^b   Kuprin, S ^a   Wrangel, M ^a   Almstedt, A ^a   Persson, P ^a   Ek, V ^a   Mikaelsson, M ^c  

^a Biovitrum AB ^*  (Sweden)

^b BIELEFELD UNIVERSITY   (Germany)

^c Kabio HB   (Sweden)

Author keywords

Biotechnology; CHO cells; Factor VIIII; MMP; Proteases; Recombinant proteins

Indexed keywords

BIOASSAY; BIOTECHNOLOGY; COLLAGEN; ENZYME INHIBITION;

CHINESE HAMSTER OVARY (CHO); CHO CELLS; FACTOR VIIII; METALLOPROTEINASES (MMP); PROTEASES; RECOMBINANT PROTEINS;

CELL CULTURE;

1,10 PHENANTHROLINE; 3,4 DICHLOROISOCOUMARIN; 4 (2 AMINOETHYL)BENZENESULFONYL FLUORIDE; APROTININ; BESTATIN; CHELATING AGENT; COLLAGENASE; CYSTATIN; DIPROTIN A; DIPROTIN B; EDETIC ACID; GELATINASE; HISTIDINE; IMIDAZOLE; LEUPEPTIN; MACROPHAGE ELASTASE; METALLOPROTEINASE; METALLOPROTEINASE 10; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; PEPSTATIN; PROTEINASE; PROTEINASE INHIBITOR; RECOMBINANT BLOOD CLOTTING FACTOR 8; RECOMBINANT PROTEIN; STROMELYSIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL LINE; CHO CELL; CONTROLLED STUDY; DRUG EFFECT; DRUG INHIBITION; ENZYME ACTIVITY; ENZYME ASSAY; GEL PERMEATION CHROMATOGRAPHY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STABILITY;

ANIMALS; CHELATING AGENTS; CHO CELLS; CRICETINAE; CULTURE MEDIA, SERUM-FREE; FACTOR VIII; MATRIX METALLOPROTEINASES; PROTEASE INHIBITORS; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS;

EID: 33845271173     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21057     Document Type: Article

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