Degradation of an Fc-fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

Biotechnology and Bioengineering

Volumn 104, Issue 6, 2009, Pages 1132-1141

  Robert, Flavie ^a   Bierau, Horst ^b   Rossi, Mara ^b   Agugiaro, David ^b   Soranzo, Thomas ^c   Broly, Hervé ^a   Mitchell Logean, Christine ^a  

^a MERCK SERONO S A   (Switzerland)

^b Merck Serono SpA   (Italy)

^c UNIV GRENOBLE ALPES   (France)

Author keywords

Cathepsin D; CHO cell culture; Fc fusion; Fed batch; Fragmentation; Protease

Indexed keywords

CATHEPSIN D; CHO CELL CULTURE; FC-FUSION; FED-BATCH; FRAGMENTATION; PROTEASE;

CELL CULTURE; CELL MEMBRANES; DEGRADATION; ENZYMES;

BATCH CELL CULTURE;

CATHEPSIN D; METALLOPROTEINASE; PROTEINASE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME PURIFICATION; HOST CELL; NONHUMAN; PH MEASUREMENT; PROTEIN ANALYSIS; SCREENING;

ANIMALS; CATHEPSIN D; CHO CELLS; CRICETINAE; CRICETULUS; ENZYME INHIBITORS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

EID: 70350491149     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22494     Document Type: Article

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