Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells

Protein Expression and Purification

Volumn 67, Issue 2, 2009, Pages 182-189

  Zhao, Yonghong ^a   Gutshall, Lester ^a   Jiang, Haiyan ^a   Baker, Audrey ^a   Beil, Eric ^a   Obmolova, Galina ^a   Carton, Jill ^a   Taudte, Susann ^a   Amegadzie, Bernard ^a  

^a CENTOCOR INC   (United States)

Author keywords

Antibody fragments; Fab; Papain cleavage; Transient expression

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; OLIGOPEPTIDE; PAPAIN;

ARTICLE; BIOSYNTHESIS; CELL LINE; CHEMISTRY; CIRCULAR DICHROISM; COMPARATIVE STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIGHT; METABOLISM; RADIATION SCATTERING; X RAY DIFFRACTION;

ANTIBODIES, MONOCLONAL; CELL LINE; CIRCULAR DICHROISM; HISTIDINE; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; LIGHT; OLIGOPEPTIDES; PAPAIN; RECOMBINANT FUSION PROTEINS; SCATTERING, RADIATION; X-RAY DIFFRACTION;

MAMMALIA;

EID: 67650167709     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.04.012     Document Type: Article

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