Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting

Biotechnology and Bioengineering

Volumn 108, Issue 2, 2011, Pages 386-394

  Pichler, Johannes ^a   Galosy, Sybille ^b   Mott, John ^b   Borth, Nicole ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Cell sorting; CHO cells; Host cell optimization; Secretion assay; Transient transfection

Indexed keywords

CELL SORTING; CHO CELL; HOST CELLS; SECRETION ASSAY; TRANSIENT TRANSFECTION;

ANTIBODIES; CELL CULTURE; CELLULAR NEURAL NETWORKS; GENE EXPRESSION; MACHINERY; OPTIMIZATION; PRODUCTIVITY;

CELLS;

PLASMID DNA;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CLONE; CELL SELECTION; CONTROLLED STUDY; ELECTROPORATION; EPIGENETICS; GENE AMPLIFICATION; GENE MUTATION; GENETIC TRANSFECTION; GENETIC VARIABILITY; HOST CELL; NONHUMAN; REPRODUCIBILITY;

ANIMALS; ANTIBODIES; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPORATION; FLOW CYTOMETRY; GENE EXPRESSION; PLASMIDS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 78650204558     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22946     Document Type: Article

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