Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Biotechnology and Bioengineering

Volumn 101, Issue 5, 2008, Pages 937-945

  Chenuet, Sebastien ^a   Martinet, Danielle ^b   Besuchet Schmutz, Nathalie ^a   Wicht, Marianne ^b   Jaccard, Nicolas ^a   Bon, Anne Charlotte ^a   Derouazi, Madiha ^a,c   Hacker, David L ^a   Beckmann, Jacques S ^b   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c UNIV GRENOBLE ALPES   (France)

Author keywords

Calcium phosphate; CHO cells; Polyethylenimine; Recombinant protein; Specific productivity

Indexed keywords

ANTIBODIES; CALCIUM; CALCIUM ALLOYS; CALCIUM PHOSPHATE; CELL CULTURE; CELLS; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; LIGHT EMISSION; LUMINESCENCE; MAMMALS; MICROSCOPIC EXAMINATION; MONOCLONAL ANTIBODIES; ORGANIC ACIDS; PHOSPHATES; PRECIPITATION (CHEMICAL); PRODUCTIVITY; PROTEINS;

CELL LINES; CHINESE HAMSTER OVARIES; CHO CELLS; COPY NUMBERS; FLUORESCENCE IN SITU HYBRIDIZATIONS; GFP EXPRESSIONS; GREEN FLUORESCENT PROTEINS; IN CELLS; METAPHASE CHROMOSOMES; POLYETHYLENIMINE; POSITIVE CELLS; PROTEIN PRODUCTIONS; RECOMBINANT ANTIBODIES; RECOMBINANT CELLS; RECOMBINANT PROTEIN; REPORTER PROTEINS; SELECTIVE PRESSURES; SINGLE SITES; SPECIFIC PRODUCTIVITY; TRANSFECTION METHODS;

NUCLEIC ACIDS;

CALCIUM PHOSPHATE; GREEN FLUORESCENT PROTEIN; MONOCLONAL ANTIBODY; POLYETHYLENEIMINE; RECOMBINANT ANTIBODY;

ANIMAL CELL; ARTICLE; CHO CELL; CHROMOSOME; CONTROLLED STUDY; EXPRESSION VECTOR; FEASIBILITY STUDY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC TRANSFECTION; MAMMAL CELL; METAPHASE; NONHUMAN; PLASMID; PRECIPITATION; PROTEIN STABILITY;

ANIMALS; CALCIUM PHOSPHATES; CHO CELLS; CRICETINAE; CRICETULUS; DNA; FEMALE; FLOW CYTOMETRY; GENE DOSAGE; GENE EXPRESSION; GENE TARGETING; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; PLASMIDS; POLYETHYLENEIMINE; PRECIPITATION; RECOMBINANT PROTEINS; TRANSFECTION; TRANSGENES;

CRICETULUS GRISEUS; MAMMALIA;

EID: 56449091807     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21972     Document Type: Article

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