A potential of microRNAs for high-content screening

Journal of Nucleic Acids

Volumn 2011, Issue , 2011, Pages

  Serva, Andrius ^a   Claas, Christoph ^a   Starkuviene, Vytaute ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; MICRORNA; RNA PRECURSOR; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; SMALL UNTRANSLATED RNA;

ASSAY; BIOGENESIS; FLUORESCENCE MICROSCOPY; FUNCTIONAL ASSESSMENT; GENE CONTROL; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE LOSS; GENE OVEREXPRESSION; GENE SILENCING; GENETIC DISORDER; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; HUMAN; MOLECULAR BIOLOGY; MOLECULAR GENETICS; MOLECULAR INTERACTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA ANALYSIS; RNA BINDING; RNA DEGRADATION; RNA INTERFERENCE; RNA PROCESSING;

EID: 84860426329     PISSN: 20900201     EISSN: 2090021X     Source Type: Journal    
DOI: 10.4061/2011/870903     Document Type: Review

References (162)

1. Flynt A. S., Lai E. C., Biological principles of microRNA-mediated regulation: shared themes amid diversity. Nature Reviews Genetics 2008 9 11 831 842 2-s2.0-54149095553 10.1038/nrg2455
2. Carthew R. W., Sontheimer E. J., Origins and mechanisms of miRNAs and siRNAs. Cell 2009 136 4 642 655 2-s2.0-60149088848 10.1016/j.cell.2009.01.035
3. Friedman R. C., Farh K. K., Burge C. B., Bartel D. P., Most mammalian mRNAs are conserved targets of microRNAs. Genome Research 2009 19 1 92 105 2-s2.0-60149095444 10.1101/gr.082701.108
4. Lewis B. P., Burge C. B., Bartel D. P., Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005 120 1 15 20 2-s2.0-11844278458 10.1016/j.cell.2004.12.035 (Pubitemid 40094598)
5. Brennecke J., Hipfner D. R., Stark A., Russell R. B., Cohen S. M., bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 2003 113 1 25 36 2-s2.0-0037418839 10.1016/S0092-8674(03)00231-9 (Pubitemid 36411957)
6. Hackl M., Brunner S., Fortschegger K., Schreiner C., Micutkova L., Mück C., Laschober G. T., Lepperdinger G., Sampson N., Berger P., Herndler-Brandstetter D., Wieser M., Kühnel H., Strasser A., Rinnerthaler M., Breitenbach M., Mildner M., Eckhart L., Tschachler E., Trost A., Bauer J. W., Papak C., Trajanoski Z., Scheideler M., Grillari-Voglauer R., Grubeck-Loebenstein B., Jansen-ürr P., Grillari J., miR-17, miR-19b, miR-20a, and miR-106a are down-regulated in human aging. Aging Cell 2010 9 2 291 296 2-s2.0-77952392153 10.1111/j.1474-9726.2010.00549.x
7. Huang X., Ding L., Bennewith K. L., Tong R. T., Welford S. M., Ang K. K., Story M., Le Q. T., Giaccia A. J., Hypoxia-inducible mir-210 regulates normoxic gene expression involved in tumor initiation. Molecular Cell 2009 35 6 856 867 2-s2.0-70349168446 10.1016/j.molcel.2009.09.006
8. Johnson C. D., Esquela-Kerscher A., Stefani G., Byrom M., Kelnar K., Ovcharenko D., Wilson M., Wang X., Shelton J., Shingara J., Chin L., Brown D., Slack F. J., The let-7 microRNA represses cell proliferation pathways in human cells. Cancer Research 2007 67 16 7713 7722 2-s2.0-34548012848 10.1158/0008-5472.CAN-07-1083 (Pubitemid 47281364)
9. Yu Z., Wang C., Wang M., Li Z., Casimiro M. C., Liu M., Wu K., Whittle J., Ju X., Hyslop T., McCue P., Pestell R. G., A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation. Journal of Cell Biology 2008 182 3 509 517 2-s2.0-49749086530 10.1083/jcb.200801079
10. Hu W., Chan C. S., Wu R., Zhang C., Sun Y., Song J. S., Tang L. H., Levine A. J., Feng Z., Negative regulation of tumor suppressor p53 by microRNA miR-504. Molecular Cell 2010 38 5 689 699 2-s2.0-77953096272 10.1016/j.molcel.2010.05.027
11. Nakano H., Miyazawa T., Kinoshita K., Yamada Y., Yoshida T., Functional screening identifies a microRNA, miR-491 that induces apoptosis by targeting Bcl-X(L)in colorectal cancer cells. International Journal of Cancer 2010 127 5 1072 1080 2-s2.0-77955492383 10.1002/ijc.25143
12. Pandey A. K., Agarwal P., Kaur K., Datta M., MicroRNAs in diabetes: tiny players in big disease. Cellular Physiology and Biochemistry 2009 23 4-6 221 232 2-s2.0-66749146650 10.1159/000218169
13. Ling H. Y., Ou H. S., Feng S. D., Zhang X. Y., Tuo Q. H., Chen L. X., Zhu B. Y., Gao Z. P., Tang C. K., Yin W. D., Zhang L., Liao D. F., Changes in microrna (mir) profile and effects of mir-320 in insulin-resistant 3t3-l1 adipocytes. Clinical and Experimental Pharmacology and Physiology 2009 36 9 e32 e39 2-s2.0-69949099167 10.1111/j.1440-1681.2009.05207.x
14. Tang X., Tang G., Özcan S., Role of microRNAs in diabetes. Biochimica et Biophysica Acta 2008 1779 11 697 701 10.1016/j.bbagrm.2008.06.010
15. Croce C. M., Causes and consequences of microRNA dysregulation in cancer. Nature Reviews Genetics 2009 10 10 704 714 2-s2.0-70349320158 10.1038/nrg2634
16. Myatt S. S., Wang J., Monteiro L. J., Christian M., Ho K. K., Fusi L., Dina R. E., Brosens J. J., Ghaem-Maghami S., Lam E. W., Definition of microRNAs that repress expression of the tumor suppressor gene FOXO1 in endometrial cancer. Cancer Research 2010 70 1 367 377 2-s2.0-75149112667 10.1158/0008-5472.CAN-09-1891
17. Jopling C. L., Yi M., Lancaster A. M., Lemon S. M., Sarnow P., Molecular biology: modulation of hepatitis C virus RNA abundance by a liver-specific microRNA. Science 2005 309 5740 1577 1581 2-s2.0-24644483623 10.1126/science.1113329 (Pubitemid 41266486)
18. Chang S., Wen S., Chen D., Jin P., Small regulatory RNAs in neurodevelopmental disorders. Human Molecular Genetics 2009 18 1 R18 R26 2-s2.0-63149101534 10.1093/hmg/ddp072
19. Xu Y., Li F., Zhang B., Zhang K., Zhang F., Huang X., Sun N., Ren Y., Sui M., Liu P., MicroRNAs and target site screening reveals a pre-microRNA-30e variant associated with schizophrenia. Schizophrenia Research 2010 119 1-3 219 227 2-s2.0-77952670366 10.1016/j.schres.2010.02.1070
20. Takeshita F., Patrawala L., Osaki M., Takahashi R. U., Yamamoto Y., Kosaka N., Kawamata M., Kelnar K., Bader A. G., Brown D., Ochiya T., Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genes. Molecular Therapy 2010 18 1 181 187 2-s2.0-74149083873 10.1038/mt.2009.207
21. Kota J., Chivukula R. R., O'Donnell K. A., Wentzel E. A., Montgomery C. L., Hwang H. W., Chang T. C., Vivekanandan P., Torbenson M., Clark K. R., Mendell J. R., Mendell J. T., Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell 2009 137 6 1005 1017 2-s2.0-66449136951 10.1016/j.cell.2009.04.021
22. Volinia S., Calin G. A., Liu C. G., Ambs S., Cimmino A., Petrocca F., Visone R., Iorio M., Roldo C., Ferracin M., Prueitt R. L., Yanaihara N., Lanza G., Scarpa A., Vecchione A., Negrini M., Harris C. C., Croce C. M., A microRNA expression signature of human solid tumors defines cancer gene targets. Proceedings of the National Academy of Sciences of the United States of America 2006 103 7 2257 2261 2-s2.0-33144490646 10.1073/pnas.0510565103 (Pubitemid 43271657)
23. Toffanin S., Hoshida Y., Lachenmayer A., Villanueva A., Cabellos L., Minguez B., Savic R., Ward S. C., Thung S., Chiang D. Y., Alsinet C., Tovar V., Roayaie S., Schwartz M., Bruix J., Waxman S., Friedman S. L., Golub T., Mazzaferro V., Llovet J. M., MicroRNA-based classification of hepatocellular carcinoma and oncogenic role of miR-517a. Gastroenterology 2011 140 5 1618 1628.e16 10.1053/j.gastro.2011.02.009
24. Huntzinger E., Izaurralde E., Gene silencing by microRNAs: contributions of translational repression and mRNA decay. Nature Reviews Genetics 2011 12 2 99 110 10.1038/nrg2936
25. Krol J., Loedige I., Filipowicz W., The widespread regulation of microRNA biogenesis, function and decay. Nature Reviews Genetics 2010 11 9 597 610 2-s2.0-77955902024 10.1038/nrg2843
26. Denli A. M., Tops B. B., Plasterk R. H., Ketting R. F., Hannon G. J., Processing of primary microRNAs by the microprocessor complex. Nature 2004 432 7014 231 235 2-s2.0-9144224451 10.1038/nature03049 (Pubitemid 39545854)
27. Yi R., Qin Y., Macara I. G., Cullen B. R., Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes and Development 2003 17 24 3011 3016 2-s2.0-0347361541 10.1101/gad.1158803 (Pubitemid 38040764)
28. Lee Y., Hur I., Park S.-Y., Kim Y.-K., Suh M. R., Kim V. N., The role of PACT in the RNA silencing pathway. EMBO Journal 2006 25 3 522 532 10.1038/sj.emboj.7600942 (Pubitemid 43237659)
29. Chendrimada T. P., Gregory R. I., Kumaraswamy E., Norman J., Cooch N., Nishikura K., Shiekhattar R., TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature 2005 436 7051 740 744 2-s2.0-23644433363 10.1038/nature03868 (Pubitemid 41117280)
30. Salzman D. W., Shubert-Coleman J., Furneaux H., P68 RNA helicase unwinds the human let-7 microRNA precursor duplex and is required for let-7-directed silencing of gene expression. The Journal of Biological Chemistry 2007 282 45 32773 32779 2-s2.0-36348979175 10.1074/jbc.M705054200 (Pubitemid 350159271)
31. Robb G. B., Rana T. M., RNA helicase a interacts with RISC in human cells and functions in RISC loading. Molecular Cell 2007 26 4 523 537 2-s2.0-34248576287 10.1016/j.molcel.2007.04.016 (Pubitemid 46765183)
32. Yang J. S., Phillips M. D., Betel D., Mu P., Ventura A., Siepel A. C., Chen K. C., Lai E. C., Widespread regulatory activity of vertebrate microRNA species. RNA 2011 17 2 312 326 10.1261/rna.2537911
33. Meister G., Landthaler M., Patkaniowska A., Dorsett Y., Teng G., Tuschl T., Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Molecular Cell 2004 15 2 185 197 2-s2.0-3242736704 10.1016/j.molcel.2004.07.007 (Pubitemid 38950966)
34. Eulalio A., Huntzinger E., Izaurralde E., GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nature Structural and Molecular Biology 2008 15 4 346 353 2-s2.0-41649115420 10.1038/nsmb.1405 (Pubitemid 351483394)
35. Winter J., Jung S., Keller S., Gregory R. I., Diederichs S., Many roads to maturity: microRNA biogenesis pathways and their regulation. Nature Cell Biology 2009 11 3 228 234 2-s2.0-61849137222 10.1038/ncb0309-228
36. Cheloufi S., Dos Santos C. O., Chong M. M., Hannon G. J., A dicer-independent miRNA biogenesis pathway that requires Ago catalysis. Nature 2010 465 7298 584 589 2-s2.0-77953183812 10.1038/nature09092
37. Cifuentes D., Xue H., Taylor D. W., Patnode H., Mishima Y., Cheloufi S., Ma E., Mane S., Hannon G. J., Lawson N. D., Wolfe S. A., Giraldez A. J., A novel miRNA processing pathway independent of dicer requires Argonaute2 catalytic activity. Science 2010 328 5986 1694 1698 2-s2.0-77953897182 10.1126/science.1190809
38. Park S. Y., Lee J. H., Ha M., Nam J. W., Kim V. N., miR-29 miRNAs activate p53 by targeting p85 α and CDC42. Nature Structural and Molecular Biology 2009 16 1 23 29 2-s2.0-58149215802 10.1038/nsmb.1533
39. Tian S., Huang S., Wu S., Guo W., Li J., He X., MicroRNA-1285 inhibits the expression of p53 by directly targeting its 3' untranslated region. Biochemical and Biophysical Research Communications 2010 396 2 435 439 2-s2.0-77952875310 10.1016/j.bbrc.2010.04.112
40. Voorhoeve P. M., le Sage C., Schrier M., Gillis A. J. M., Stoop H., Nagel R., Liu Y. P., van Duijse J., Drost J., Griekspoor A., Zlotorynski E., Yabuta N., De Vita G., Nojima H., Looijenga L. H. J., Agami R., A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 2006 124 6 1169 1181 2-s2.0-33646056975 10.1016/j.cell.2006.02.037
41. Baek D., Villén J., Shin C., Camargo F. D., Gygi S. P., Bartel D. P., The impact of microRNAs on protein output. Nature 2008 455 7209 64 71 2-s2.0-49949116902 10.1038/nature07242
42. Selbach M., Schwanhäusser B., Thierfelder N., Fang Z., Khanin R., Rajewsky N., Widespread changes in protein synthesis induced by microRNAs. Nature 2008 455 7209 58 63 2-s2.0-49949117302 10.1038/nature07228
43. Bushati N., Cohen S. M., MicroRNA functions. Annual Review of Cell and Developmental Biology 2007 23 175 205 2-s2.0-34547960488 10.1146/annurev. cellbio.23.090506.123406
44. Du L., Schageman J. J., Subauste M. C., Saber B., Hammond S. M., Prudkin L., Wistuba I. I., Ji L., Roth J. A., Minna J. D., Pertsemlidis A., MiR-93, miR-98, and miR-197 regulate expression of tumor suppressor gene FUS1. Molecular Cancer Research 2009 7 8 1234 1243 2-s2.0-69449105719 10.1158/1541-7786.MCR-08- 0507
45. Kip1 tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation. The EMBO Journal 2007 26 15 3699 3708 2-s2.0-34547791273 10.1038/sj.emboj.7601790 (Pubitemid 47236875)
46. Wu S., Huang S., Ding J., Zhao Y., Liang L., Liu T., Zhan R., He X., Multiple microRNAs modulate p21Cip1/Waf1 expression by directly targeting its 3′ untranslated region. Oncogene 2010 29 15 2302 2308 2-s2.0-77951092028 10.1038/onc.2010.34
47. Pillai R. S., MicroRNA function: multiple mechanisms for a tiny RNA? RNA 2005 11 12 1753 1761 2-s2.0-28344440823 10.1261/rna.2248605 (Pubitemid 41720024)
48. Lee I., Ajay S. S., Yook J. I., Hyun S. K., Kim H. S., Hong S. H., Kim N. H., Dhanasekaran S. M., Chinnaiyan A., Athey B. D., New class of microRNA targets containing simultaneous 5′ -UTR and 3′ -UTR interaction sites. Genome Research 2009 19 7 1175 1183 2-s2.0-67650087599 10.1101/gr.089367.108
49. Elcheva I., Goswami S., Noubissi F. K., Spiegelman V. S., CRD-BP protects the coding region of β TrCP1 mRNA from miR-183-mediated degradation. Molecular Cell 2009 35 2 240 246 2-s2.0-67651083537 10.1016/j.molcel.2009.06.007
50. Lee R. C., Feinbaum R. L., Ambros V., The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 1993 75 5 843 854 2-s2.0-0027751663 10.1016/0092-8674(93)90529-Y (Pubitemid 24014542)
51. Fire A., Xu S., Montgomery M. K., Kostas S. A., Driver S. E., Mello C. C., Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 1998 391 6669 806 811 2-s2.0-0032545933 10.1038/35888 (Pubitemid 28099681)
52. Meister G., Tuschl T., Mechanisms of gene silencing by double-stranded RNA. Nature 2004 431 7006 343 349 2-s2.0-4644223259 10.1038/nature02873 (Pubitemid 39265674)
53. Mello C. C., Conte D. Jr., Revealing the world of RNA interference. Nature 2004 431 7006 338 342 2-s2.0-4644336023 10.1038/nature02872 (Pubitemid 39265673)
54. Tomari Y., Zamore P. D., Perspective: machines for RNAi. Genes and Development 2005 19 5 517 529 2-s2.0-14644393684 10.1101/gad.1284105 (Pubitemid 40314984)
55. Hammond S. M., Bernstein E., Beach D., Hannon G. J., An RNA-directed nuclease mediates post-transcriptional gene silencing in cells. Nature 2000 404 6775 293 296 2-s2.0-0034673638 10.1038/35005107 (Pubitemid 30163547)
56. Zeng Y., Yi R., Cullen B. R., MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proceedings of the National Academy of Sciences of the United States of America 2003 100 17 9779 9784 2-s2.0-0041691111 10.1073/pnas.1630797100 (Pubitemid 37087018)
57. Filipowicz W., Bhattacharyya S. N., Sonenberg N., Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature Reviews Genetics 2008 9 2 102 114 2-s2.0-38349169664 10.1038/nrg2290
58. Collinet C., Stöter M., Bradshaw C. R., Samusik N., Rink J. C., Kenski D., Habermann B., Buchholz F., Henschel R., Mueller M. S., Nagel W. E., Fava E., Kalaidzidis Y., Zerial M., Systems survey of endocytosis by multiparametric image analysis. Nature 2010 464 7286 243 249 2-s2.0-77949424084 10.1038/nature08779
59. Neumann B., Walter T., Hériché J. K., Bulkescher J., Erfle H., Conrad C., Rogers P., Poser I., Held M., Liebel U., Cetin C., Sieckmann F., Pau G., Kabbe R., Wünsche A., Satagopam V., Schmitz M. H. A., Chapuis C., Gerlich D. W., Schneider R., Eils R., Huber W., Peters J. M., Hyman A. A., Durbin R., Pepperkok R., Ellenberg J., Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 2010 464 7289 721 727 2-s2.0-77950387346 10.1038/nature08869
60. Boutros M., Ahringer J., The art and design of genetic screens: RNA interference. Nature Reviews Genetics 2008 9 7 554 566 2-s2.0-45549089565 10.1038/nrg2364 (Pubitemid 351861541)
61. Timmons L., Court D. L., Fire A., Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 2001 263 1-2 103 112 2-s2.0-0035941485 10.1016/S0378-1119(00) 00579-5 (Pubitemid 32167059)
62. Timmons L., Fire A., Specific interference by ingested dsRNA. Nature 1998 395 6705 854 2-s2.0-0032578911 10.1038/27579 (Pubitemid 28503418)
63. Clemens J. C., Worby C. A., Simonson-Leff N., Muda M., Maehama T., Hemmings B. A., Dixon J. E., Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proceedings of the National Academy of Sciences of the United States of America 2000 97 12 6499 6503 2-s2.0-0034612276 10.1073/pnas.110149597 (Pubitemid 30412742)
64. Elbashir S. M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T., Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001 411 6836 494 498 2-s2.0-0035942736 10.1038/35078107 (Pubitemid 32494397)
65. Kittler R., Pelletier L., Heninger A. K., Slabicki M., Theis M., Miroslaw L., Poser I., Lawo S., Grabner H., Kozak K., Wagner J., Surendranath V., Richter C., Bowen W., Jackson A. L., Habermann B., Hyman A. A., Buchholz F., Genome-scale RNAi profiling of cell division in human tissue culture cells. Nature Cell Biology 2007 9 12 1401 1412 2-s2.0-36749016685 10.1038/ncb1659 (Pubitemid 350201753)
66. König R., Chiang C. Y., Tu B. P., Yan S. F., DeJesus P. D., Romero A., Bergauer T., Orth A., Krueger U., Zhou Y., Chanda S. K., A probability-based approach for the analysis of large-scale RNAi screens. Nature Methods 2007 4 10 847 849 2-s2.0-35848929692 10.1038/nmeth1089 (Pubitemid 350055583)
67. Paddison P. J., Silva J. M., Conklin D. S., Schlabach M., Li M., Aruleba S., Balija V., O'Shaughnessy A., Gnoj L., Scoble K., Chang K., Westbrook T., Cleary M., Sachidanandam R., McComble W. R., Elledge S. J., Hannon G. J., A resource for large-scale RNA-interference-based screens in mammals. Nature 2004 428 6981 427 431 2-s2.0-12144288006 10.1038/nature02370 (Pubitemid 38419688)
68. Berns K., Hijmans E. M., Mullenders J., Brummelkamp T. R., Velds A., Heimerikx M., Kerkhoven R. M., Madlredjo M., Nijkamp W., Weigelt B., Agami R., Ge W., Cavet G., Linsley P. S., Beijersbergen R. L., Bernards R., A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 2004 428 6981 431 437 2-s2.0-12144289681 10.1038/nature02371 (Pubitemid 38419689)
69. Moffat J., Grueneberg D. A., Yang X., Kim S. Y., Kloepfer A. M., Hinkle G., Piqani B., Eisenhaure T. M., Luo B., Grenier J. K., Carpenter A. E., Foo S. Y., Stewart S. A., Stockwell B. R., Hacohen N., Hahn W. C., Lander E. S., Sabatini D. M., Root D. E., A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 2006 124 6 1283 1298 2-s2.0-33646033137 10.1016/j.cell.2006.01.040
70. Silva J. M., Li M. Z., Chang K., Ge W., Golding M. C., Rickles R. J., Siolas D., Hu G., Paddison P. J., Schlabach M. R., Sheth N., Bradshaw J., Burchard J., Kulkarni A., Cavet G., Sachidanandam R., McCombie W. R., Cleary M. A., Elledge S. J., Hannon G. J., Second-generation shRNA libraries covering the mouse and human genomes. Nature Genetics 2005 37 11 1281 1288 2-s2.0-27644436787 10.1038/ng1650 (Pubitemid 41568714)
71. Garofalo M., Croce C. M., MicroRNAs: master regulators as potential therapeutics in cancer. Annual Review of Pharmacology and Toxicology 2011 51 25 43 10.1146/annurev-pharmtox-010510-100517
72. Mavrakis K. J., Wolfe A. L., Oricchio E., Palomero T., de Keersmaecker K., McJunkin K., Zuber J., James T., Chang K., Khan A. A., Leslie C. S., Parker J. S., Paddison P. J., Tam W., Ferrando A., Wendel H. G., Genome-wide RNA-mediated interference screen identifies miR-19 targets in notch-induced T-cell acute lymphoblastic leukaemia. Nature Cell Biology 2010 12 4 372 379 2-s2.0-77950518606 10.1038/ncb2037
73. Bartel D. P., MicroRNAs: target recognition and regulatory functions. Cell 2009 136 2 215 233 2-s2.0-58249088751 10.1016/j.cell.2009.01.002
74. Krek A., Grün D., Poy M. N., Wolf R., Rosenberg L., Epstein E. J., MacMenamin P., da Piedade I., Gunsalus K. C., Stoffel M., Rajewsky N., Combinatorial microRNA target predictions. Nature Genetics 2005 37 5 495 500 2-s2.0-20944450160 10.1038/ng1536 (Pubitemid 40617277)
75. John B., Enright A. J., Aravin A., Tuschl T., Sander C., Marks D. S., Human microRNA targets. PLoS Biology 2004 2 11, article e363 2-s2.0-14044251458 10.1371/journal.pbio.0020363 (Pubitemid 40280484)
76. Xie X., Lu J., Kulbokas E. J., Golub T. R., Mootha V., Lindblad-Toh K., Lander E. S., Kellis M., Systematic discovery of regulatory motifs in human promoters and 3' UTRs by comparison of several mammals. Nature 2005 434 7031 338 345 2-s2.0-15244358503 10.1038/nature03441 (Pubitemid 40469170)
77. Capecchi M. R., Generating mice with targeted mutations. Nature Medicine 2001 7 10 1086 1090 2-s2.0-0034770472 10.1038/nm1001-1086 (Pubitemid 33010007)
78. Tong C., Li P., Wu N. L., Yan Y., Ying Q. L., Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 2010 467 7312 211 213 2-s2.0-77955289253 10.1038/nature09368
79. Urnov F. D., Rebar E. J., Holmes M. C., Zhang H. S., Gregory P. D., Genome editing with engineered zinc finger nucleases. Nature Reviews Genetics 2010 11 9 636 646 2-s2.0-77955867185 10.1038/nrg2842
80. Zamecnik P. C., Stephenson M. L., Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide. Proceedings of the National Academy of Sciences of the United States of America 1978 75 1 280 284 2-s2.0-0041582974 (Pubitemid 8290783)
81. Kurreck J., Antisense technologies: improvement through novel chemical modifications. European Journal of Biochemistry 2003 270 8 1628 1644 2-s2.0-0037733977 10.1046/j.1432-1033.2003.03555.x (Pubitemid 36532522)
82. Vickers T. A., Koo S., Bennett C. F., Crooke S. T., Dean N. M., Baker B. F., Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis. The Journal of Biological Chemistry 2003 278 9 7108 7118 2-s2.0-0037470145 10.1074/jbc.M210326200 (Pubitemid 36800708)
83. Krützfeldt J., Kuwajima S., Braich R., Rajeev K. G., Pena J., Tuschl T., Manoharan M., Stoffel M., Specificity, duplex degradation and subcellular localization of antagomirs. Nucleic Acids Research 2007 35 9 2885 2892 2-s2.0-34250679405 10.1093/nar/gkm024 (Pubitemid 47073627)
84. Elmén J., Lindow M., Silahtaroglu A., Bak M., Christensen M., Lind-Thomsen A., Hedtjärn M., Hansen J. B., Hansen H. F., Straarup E. M., McCullagh K., Kearney P., Kauppinen S., Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver. Nucleic Acids Research 2008 36 4 1153 1162 2-s2.0-40249106014 10.1093/nar/gkm1113 (Pubitemid 351330933)
85. Davis S., Propp S., Freier S. M., Jones L. E., Serra M. J., Kinberger G., Bhat B., Swayze E. E., Bennett C. F., Esau C., Potent inhibition of microRNA in vivo without degradation. Nucleic Acids Research 2009 37 1 70 77 2-s2.0-58549109007 10.1093/nar/gkn904
86. Lu Y., Xiao J., Lin H., Bai Y., Luo X., Wang Z., Yang B., A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for microRNA interference. Nucleic Acids Research 2009 37 3, article e24 2-s2.0-63349085394 10.1093/nar/gkn1053
87. Esau C. C., Inhibition of microRNA with antisense oligonucleotides. Methods 2008 44 1 55 60 2-s2.0-37349113774 10.1016/j.ymeth.2007.11.001 (Pubitemid 350297990)
88. Kloosterman W. P., Lagendijk A. K., Ketting R. F., Moulton J. D., Plasterk R. H., Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biology 2007 5 8, article e203 2-s2.0-37349055535 10.1371/journal.pbio.0050203 (Pubitemid 47328277)
89. Lee Y. S., Kim H. K., Chung S., Kim K. S., Dutta A., Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation. The Journal of Biological Chemistry 2005 280 17 16635 16641 2-s2.0-20444440706 10.1074/jbc.M412247200 (Pubitemid 41389120)
90. Eisen J. S., Smith J. C., Controlling morpholino experiments: don't stop making antisense. Development 2008 135 10 1735 1743 2-s2.0-49949086671 10.1242/dev.001115
91. Ebert M. S., Neilson J. R., Sharp P. A., MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nature Methods 2007 4 9 721 726 2-s2.0-34548316982 10.1038/nmeth1079 (Pubitemid 47338166)
92. Loya C. M., Lu C. S., Van Vactor D., Fulga T. A., Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. Nature Methods 2009 6 12 897 903 2-s2.0-73349122330 10.1038/nmeth.1402
93. Tsuda N., Ishiyama S., Li Y., Ioannides C. G., Abbruzzese J. L., Chang D. Z., Synthetic microRNA designed to target glioma-associated antigen 1 transcription factor inhibits division and induces late apoptosis in pancreatic tumor cells. Clinical Cancer Research 2006 12 21 6557 6564 2-s2.0-33751264968 10.1158/1078-0432.CCR-06-0588 (Pubitemid 44799731)
94. Garzon R., Heaphy C. E., Havelange V., Fabbri M., Volinia S., Tsao T., Zanesi N., Kornblau S. M., Marcucci G., Calin G. A., Andreeff M., Croce C. M., MicroRNA 29b functions in acute myeloid leukemia. Blood 2009 114 26 5331 5341 2-s2.0-73949118737 10.1182/blood-2009-03-211938
95. Chung K. H., Hart C. C., Al-Bassam S., Avery A., Taylor J., Patel P. D., Vojtek A. B., Turner D. L., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Research 2006 34 7 e53 2-s2.0-33645804365 10.1093/nar/gkl143 (Pubitemid 44314213)
96. Kumar D., An C. I., Yokobayashi Y., Conditional RNA interference mediated by allosteric ribozyme. Journal of the American Chemical Society 2009 131 39 13906 13907 2-s2.0-70349631634 10.1021/ja905596t
97. Schmidt-Supprian M., Rajewsky K., Vagaries of conditional gene targeting. Nature Immunology 2007 8 7 665 668 2-s2.0-34250694086 10.1038/ni0707-665 (Pubitemid 46965339)
98. Choi W. Y., Giraldez A. J., Schier A. F., Target protectors reveal dampening and balancing of nodal agonist and antagonist by miR-430. Science 2007 318 5848 271 274 2-s2.0-35348914716 10.1126/science.1147535 (Pubitemid 47574854)
99. Lam L. T., Lu X., Zhang H., Lesniewski R. R., Rosenberg S. H., Semizarov D., A microRNA screen to identify modulators of sensitivity to BCL2 inhibitor ABT-263 (navitoclax). Molecular Cancer Therapeutics 2010 9 11 2943 2950 2-s2.0-78649663246 10.1158/1535-7163.MCT-10-0427
100. Cheng A. M., Byrom M. W., Shelton J., Ford L. P., Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Research 2005 33 4 1290 1297 2-s2.0-14844364472 10.1093/nar/gki200 (Pubitemid 41439922)
101. Huang Q., Gumireddy K., Schrier M., le Sage C., Nagel R., Nair S., Egan D. A., Li A., Huang G., Klein-Szanto A. J., Gimotty P. A., Katsaros D., Coukos G., Zhang L., Puré E., Agami R., The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis. Nature Cell Biology 2008 10 2 202 210 2-s2.0-38849107424 10.1038/ncb1681 (Pubitemid 351194050)
102. Nagel R., Clijsters L., Agami R., The miRNA-192/194 cluster regulates the Period gene family and the circadian clock. FEBS Journal 2009 276 19 5447 5455 2-s2.0-70349245117 10.1111/j.1742-4658.2009.07229.x
103. Subramanian T., Chinnadurai G., Temperature-sensitive replication-competent adenovirus shRNA vectors to study cellular genes in virus-induced apoptosis. Methods in Molecular Medicine 2007 130 125 134 2-s2.0-34547474547
104. Ovcharenko D., Kelnar K., Johnson C., Leng N., Brown D., Genome-scale microRNA and small interfering RNA screens identify small RNA modulators of TRAIL-induced apoptosis pathway. Cancer Research 2007 67 22 10782 10788 2-s2.0-36349012607 10.1158/0008-5472.CAN-07-1484 (Pubitemid 350145906)
105. Whittaker R., Loy P. A., Sisman E., Suyama E., Aza-Blanc P., Ingermanson R. S., Price J. H., McDonough P. M., Identification of microRNAs that control lipid droplet formation and growth in hepatocytes via high-content screening. Journal of Biomolecular Screening 2010 15 7 798 805 2-s2.0-77956054428 10.1177/1087057110374991
106. Wightman B., Ha I., Ruvkun G., Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 1993 75 5 855 862 2-s2.0-0027730383 10.1016/0092-8674(93)90530-4 (Pubitemid 24014543)
107. Reinhart B. J., Slack F. J., Basson M., Pasquienelll A. E., Bettlnger J. C., Rougvle A. E., Horvitz H. R., Ruvkun G., The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 2000 403 6772 901 906 10.1038/35002607 (Pubitemid 30130993)
108. Pasquinelli A. E., Reinhart B. J., Slack F., Martindale M. Q., Kuroda M. I., Maller B., Hayward D. C., Ball E. E., Degnan B., Müller P., Spring J., Srinivasan A., Fishman M., Finnerty J., Corbo J., Levine M., Leahy P., Davidson E., Ruvkun G., Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 2000 408 6808 86 89 2-s2.0-0034597777 10.1038/35040556
109. Aza-Blanc P., Cooper C. L., Wagner K., Batalov S., Deveraux Q. L., Cooke M. P., Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Molecular Cell 2003 12 3 627 637 2-s2.0-0141750434 10.1016/S1097-2765(03)00348-4 (Pubitemid 37222485)
110. Silver S. J., Hagen J. W., Okamura K., Perrimon N., Lai E. C., Functional screening identifies miR-315 as a potent activator of Wingless signaling. Proceedings of the National Academy of Sciences of the United States of America 2007 104 46 18151 18156 2-s2.0-36749036712 10.1073/pnas.0706673104 (Pubitemid 350210836)
111. Cole K. A., Attiyeh E. F., Mosse Y. P., Laquaglia M. J., Diskin S. J., Brodeur G. M., Maris J. M., A functional screen identifies miR-34a as a candidate neuroblastoma tumor suppressor gene. Molecular Cancer Research 2008 6 5 735 742 2-s2.0-49749150980 10.1158/1541-7786.MCR-07-2102
112. Sirotkin A. V., Ovcharenko D., Grossmann R., Lauková M., Mlynek M., Identification of microRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen. Journal of Cellular Physiology 2009 219 2 415 420 10.1002/jcp.21689
113. Sirotkin A. V., Lauková M., Ovcharenko D., Brenaut P., Mlynek M., Identification of microRNAs controlling human ovarian cell proliferation and apoptosis. Journal of Cellular Physiology 2010 223 1 49 56 10.1002/jcp.21999
114. Borgdorff V., Lleonart M. E., Bishop C. L., Fessart D., Bergin A. H., Overhoff M. G., Beach D. H., Multiple microRNAs rescue from Ras-induced senescence by inhibiting p 21 Waf1/Cip1. Oncogene 2010 29 15 2262 2271 2-s2.0-77951054607 10.1038/onc.2009.497
115. Izumiya M., Okamoto K., Tsuchiya N., Nakagama H., Functional screening using a microRNA virus library and microarrays: a new high-throughput assay to identify tumor-suppressive microRNAs. Carcinogenesis 2010 31 8 1354 1359 2-s2.0-77955342492 10.1093/carcin/bgq112
116. Serva A., Knapp B., Claas C., MiR-17 family regulates cargo trafficking. in review
117. Sylvestre Y., De Guire V., Querido E., Mukhopadhyay U. K., Bourdeau V., Major F., Ferbeyre G., Chartrand P., An E2F/miR-20a autoregulatory feedback loop. The Journal of Biological Chemistry 2007 282 4 2135 2143 2-s2.0-34047267329 10.1074/jbc.M608939200 (Pubitemid 47076740)
118. Kip1 The Journal of Biological Chemistry 2007 282 32 23716 23724 2-s2.0-34548168073 10.1074/jbc.M701805200 (Pubitemid 47311902)
119. Akao Y., Nakagawa Y., Naoe T., let-7 functions as a potential growth suppressor in human colon cancer cells. Biological and Pharmaceutical Bulletin 2006 29 5 903 906 10.1248/bpb.29.903
120. Felli N., Fontana L., Pelosi E., Botta R., Bonci D., Facchiano F., Liuzzi F., Lulli V., Morsilli O., Santoro S., Valtieri M., Calin G. A., Liu C. -G., Sorrentino A., Croce C. M., Peschle C., MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation. Proceedings of the National Academy of Sciences of the United States of America 2005 102 50 18081 18086 10.1073/pnas.0506216102 (Pubitemid 41798505)
121. Esau C., Kang X., Peralta E., Hanson E., Marcusson E. G., Ravichandran L. V., Sun Y., Koo S., Perera R. J., Jain R., Dean N. M., Freier S. M., Bennett C. F., Lollo B., Griffey R., MicroRNA-143 regulates adipocyte differentiation. The Journal of Biological Chemistry 2004 279 50 52361 52365 2-s2.0-10344243662 10.1074/jbc.C400438200 (Pubitemid 39656612)
122. Chan J. A., Krichevsky A. M., Kosik K. S., MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Research 2005 65 14 6029 6033 2-s2.0-22244467087 10.1158/0008-5472.CAN-05-0137 (Pubitemid 40994386)
123. Chen J. F., Mandel E. M., Thomson J. M., Wu Q., Callis T. E., Hammond S. M., Conlon F. L., Wang D. Z., The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature Genetics 2006 38 2 228 233 2-s2.0-31744432337 10.1038/ng1725 (Pubitemid 43177239)
124. Hayashita Y., Osada H., Tatematsu Y., Yamada H., Yanagisawa K., Tomida S., Yatabe Y., Kawahara K., Sekido Y., Takahashi T., A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Research 2005 65 21 9628 9632 2-s2.0-27544495514 10.1158/0008-5472.CAN-05-2352 (Pubitemid 41541436)
125. Lu Y., Thomson J. M., Wong H. Y., Hammond S. M., Hogan B. L., Transgenic over-expression of the microRNA miR-17-92 cluster promotes proliferation and inhibits differentiation of lung epithelial progenitor cells. Developmental Biology 2007 310 2 442 453 2-s2.0-34748900267 10.1016/j.ydbio.2007.08.007 (Pubitemid 47483685)
126. Manni I., Artuso S., Careccia S., Rizzo M. G., Baserga R., Piaggio G., Sacchi A., The microRNA miR-92 increases proliferation of myeloid cells and by targeting p63 modulates the abundance of its isoforms. FASEB Journal 2009 23 11 3957 3966 2-s2.0-70350560746 10.1096/fj.09-131847
127. Pickering M. T., Stadler B. M., Kowalik T. F., MiR-17 and miR-20a temper an E2F1-induced G1 checkpoint to regulate cell cycle progression. Oncogene 2009 28 1 140 145 2-s2.0-58149328931 10.1038/onc.2008.372
128. O'Donnell K. A., Wentzel E. A., Zeller K. I., Dang C. V., Mendell J. T., c-Myc-regulated microRNAs modulate E2F1 expression. Nature 2005 435 7043 839 843 2-s2.0-20444479428 10.1038/nature03677 (Pubitemid 40839736)
129. Manion M. K., Hockenbery D. M., Targeting BCL-2-related proteins in cancer therapy. Cancer Biology Therapy 2003 2 4 S105 S114 2-s2.0-1542436707
130. Esquela-Kerscher A., Trang P., Wiggins J. F., Patrawala L., Cheng A., Ford L., Weidhaas J. B., Brown D., Bader A. G., Slack F. J., The let-7 microRNA reduces tumor growth in mouse models of lung cancer. Cell Cycle 2008 7 6 759 764 2-s2.0-42049103216 (Pubitemid 351520040)
131. Jiang L., Huang Q., Zhang S., Zhang Q., Chang J., Qiu X., Wang E., Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells. BMC Cancer 2010 10, article 318 2-s2.0-77953652759 10.1186/1471-2407-10-318
132. Meister G., Landthaler M., Dorsett Y., Tuschl T., Sequence-specific inhibition of microRNA-and siRNA-induced RNA silencing. RNA 2004 10 3 544 550 10.1261/rna.5235104 (Pubitemid 38241013)
133. Pepperkok R., Ellenberg J., High-throughput fluorescence microscopy for systems biology. Nature Reviews Molecular Cell Biology 2006 7 9 690 696 2-s2.0-33747624926 10.1038/nrm1979 (Pubitemid 44268214)
134. Sacher R., Stergiou L., Pelkmans L., Lessons from genetics: interpreting complex phenotypes in RNAi screens. Current Opinion in Cell Biology 2008 20 4 483 489 2-s2.0-47149099717 10.1016/j.ceb.2008.06.002
135. Rajewsky N., MicroRNA target predictions in animals. Nature Genetics 2006 38 1, supplement S8 S13 2-s2.0-33745248430 10.1038/ng1798
136. Martin M. M., Lee E. J., Buckenberger J. A., Schmittgen T. D., Elton T. S., MicroRNA-155 regulates human angiotensin II type 1 receptor expression in fibroblast. The Journal of Biological Chemistry 2006 281 27 18277 18284 2-s2.0-33745826614 10.1074/jbc.M601496200 (Pubitemid 44035484)
137. Brodersen P., Voinnet O., Revisiting the principles of microRNA target recognition and mode of action. Nature Reviews Molecular Cell Biology 2009 10 2 141 148 2-s2.0-58849093262 10.1038/nrm2619
138. Beitzinger M., Peters L., Zhu J. Y., Kremmer E., Meister G., Identification of human microRNA targets from isolated argonaute protein complexes. RNA Biology 2007 4 2 76 84 2-s2.0-37649021046
139. Easow G., Teleman A. A., Cohen S. M., Isolation of microRNA targets by miRNP immunopurification. RNA 2007 13 8 1198 1204 2-s2.0-34447521718 10.1261/rna.563707 (Pubitemid 47080465)
140. Karginov F. V., Conaco C., Xuan Z., Schmidt B. H., Parker J. S., Mandel G., Hannon G. J., A biochemical approach to identifying microRNA targets. Proceedings of the National Academy of Sciences of the United States of America 2007 104 49 19291 19296 2-s2.0-37648999735 10.1073/pnas.0709971104
141. Fraser A. G., Kamath R. S., Zipperlen P., Martinez-Campos M., Sohrmann M., Ahringer J., Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 2000 408 6810 325 330 2-s2.0-0034676457 10.1038/35042517
142. Kamath R. S., Fraser A. G., Dong Y., Poulin G., Durbin R., Gotta M., Kanapin A., Le Bot N., Moreno S., Sohrmann M., Welchman D. P., Zipperien P., Ahringer J., Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 2003 421 6920 231 237 2-s2.0-0037448540 10.1038/nature01278 (Pubitemid 36139121)
143. Zhou H., Xu M., Huang Q., Gates A. T., Zhang X. D., Castle J. C., Stec E., Ferrer M., Strulovici B., Hazuda D. J., Espeseth A. S., Genome-scale RNAi screen for host factors required for HIV replication. Cell Host and Microbe 2008 4 5 495 504 2-s2.0-55249088255 10.1016/j.chom.2008.10.004
144. Carpenter A. E., Sabatini D. M., Systematic genome-wide screens of gene function. Nature Reviews Genetics 2004 5 1 11 22 2-s2.0-0346728473 10.1038/nrg1248 (Pubitemid 38055332)
145. Falschlehner C., Steinbrink S., Erdmann G., Boutros M., High-throughput RNAi screening to dissect cellular pathways: a how-to guide. Biotechnology Journal 2010 5 4 368 376 2-s2.0-77950668281 10.1002/biot.200900277
146. Horn T., Sandmann T., Boutros M., Design and evaluation of genome-wide libraries for RNA interference screens. Genome Biology 2010 11 6, article R61 2-s2.0-78049437928
147. Marques J. T., Williams B. R., Activation of the mammalian immune system by siRNAs. Nature Biotechnology 2005 23 11 1399 1405 2-s2.0-27944459341 10.1038/nbt1161 (Pubitemid 41679392)
148. Judge A. D., Sood V., Shaw J. R., Fang D., McClintock K., MacLachlan I., Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nature Biotechnology 2005 23 4 457 462 2-s2.0-22344454846 10.1038/nbt1081 (Pubitemid 41724921)
149. Elmén J., Thonberg H., Ljungberg K., Frieden M., Westergaard M., Xu Y., Wahren B., Liang Z., Orum H., Koch T., Wahlestedt C., Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality. Nucleic Acids Research 2005 33 1 439 447 2-s2.0-13744250159 10.1093/nar/gki193 (Pubitemid 40276964)
150. Jackson A. L., Burchard J., Schelter J., Chau B. N., Cleary M., Lim L., Linsley P. S., Widespread siRNA off-target transcript silencing mediated by seed region sequence complementarity. RNA 2006 12 7 1179 1187 2-s2.0-33745612464 10.1261/rna.25706 (Pubitemid 43990543)
151. Echeverri C. J., Beachy P. A., Baum B., Boutros M., Buchholz F., Chanda S. K., Downward J., Ellenberg J., Fraser A. G., Hacohen N., Hahn W. C., Jackson A. L., Kiger A., Linsley P. S., Lum L., Ma Y., Mathey-Prévôt B., Root D. E., Sabatini D. M., Taipale J., Perrimon N., Bernards R., Minimizing the risk of reporting false positives in large-scale RNAi screens. Nature Methods 2006 3 10 777 779 2-s2.0-33749023011 10.1038/nmeth1006-777 (Pubitemid 44445821)
152. Echeverri C. J., Perrimon N., High-throughput RNAi screening in cultured cells: a user's guide. Nature Reviews Genetics 2006 7 5 373 384 2-s2.0-33646187810 10.1038/nrg1836
153. Grimm D., Streetz K. L., Jopling C. L., Storm T. A., Pandey K., Davis C. R., Marion P., Salazar F., Kay M. A., Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 2006 441 7092 537 541 2-s2.0-33745299723 10.1038/nature04791 (Pubitemid 44050158)
154. Jackson A. L., Linsley P. S., Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nature Reviews Drug Discovery 2010 9 1 57 67 2-s2.0-74049124186 10.1038/nrd3010
155. Stegmeier F., Hu G., Rickles R. J., Hannon G. J., Elledge S. J., A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America 2005 102 37 13212 13217 2-s2.0-24944560377 10.1073/pnas.0506306102 (Pubitemid 41325003)
156. Grimm D., Kay M. A., Therapeutic short hairpin RNA expression in the liver: viral targets and vectors. Gene Therapy 2006 13 6 563 575 2-s2.0-33645136607 10.1038/sj.gt.3302727
157. Diederichs S., Jung S., Rothenberg S. M., Smolen G. A., Mlody B. G., Haber D. A., Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites. Proceedings of the National Academy of Sciences of the United States of America 2008 105 27 9284 9289 2-s2.0-48249097174 10.1073/pnas.0800803105
158. Bartel D. P., Chen C. Z., Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs. Nature Reviews Genetics 2004 5 5 396 400 2-s2.0-1942435249 (Pubitemid 38529411)
159. Del Nery E., Miserey-Lenkei S., Falguières T., Nizak C., Johannes L., Perez F., Goud B., Rab6A and Rab6A′ GTPases play non-overlapping roles in membrane trafficking. Traffic 2006 7 4 394 407 2-s2.0-33644870151 10.1111/j.1600-0854.2006.00395.x (Pubitemid 43372749)
160. Ambros V., MicroRNAs: genetically sensitized worms reveal new secrets. Current Biology 2010 20 14 R598 R600 2-s2.0-78049522849 10.1016/j.cub.2010.05. 054
161. Miska E. A., Alvarez-Saavedra E., Abbott A. L., Lau N. C., Hellman A. B., McGonagle S. M., Bartel D. P., Ambros V. R., Horvitz H. R., Most Caenorhabditis elegans microRNAs are individually not essential for development or viability. PLoS genetics 2007 3 12, article e215 2-s2.0-44449166547 10.1371/journal.pgen. 0030215
162. Brenner J. L., Jasiewicz K. L., Fahley A. F., Kemp B. J., Abbott A. L., Loss of individual microRNAs causes mutant phenotypes in sensitized genetic backgrounds in C. elegans. Current Biology 2010 20 14 1321 1325 2-s2.0-77957756295 10.1016/j.cub.2010.05.062