High-throughput RNAi screening to dissect cellular pathways: A how-to guide

Biotechnology Journal

Volumn 5, Issue 4, 2010, Pages 368-376

  Falschlehner, Christina ^a   Steinbrink, Sandra ^a   Erdmann, Gerrit ^a   Boutros, Michael ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Cellular signaling; High throughput; Methods; RNA interference; Screening

Indexed keywords

CELLULAR PATHWAY; CELLULAR SIGNALING; DATA ANALYSIS; DESIGN AND APPLICATION; GENE FUNCTION; GENETIC SCREENING; HIGH-THROUGHPUT; HIGH-THROUGHPUT METHOD; KEY COMPONENT; MAMMALIAN CELLS; METHODS; MODEL ORGANISMS; QUANTITATIVE ASSAY; RNA INTERFERENCE; VALIDATION STRATEGIES;

MAMMALS; RNA; SIGNALING;

THROUGHPUT;

B LYMPHOCYTE RECEPTOR; COMPLEMENTARY RNA; DOUBLE STRANDED RNA; MESSENGER RNA; SMALL INTERFERING RNA; T LYMPHOCYTE RECEPTOR;

CELL LINE; CELL STRUCTURE; CELL VIABILITY; CHEMOLUMINESCENCE; DATA ANALYSIS; DROSOPHILA; GENE FUNCTION; GENE LIBRARY; GENE SILENCING; GENETIC SCREENING; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; MAMMAL CELL; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA INTERFERENCE; RNA SEQUENCE; SIGNAL TRANSDUCTION;

BETA CATENIN; GENE TARGETING; GENETIC TESTING; RNA INTERFERENCE; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION;

MAMMALIA;

EID: 77950668281     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200900277     Document Type: Review

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