An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

Nature

Volumn 404, Issue 6775, 2000, Pages 293-296

  Hammond, Scott M ^a   Bernstein, Emily ^b,d   Beach, David ^a,c   Hannon, Gregory J ^d  

^a Genetica Inc   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^d Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE;

ARTICLE; DROSOPHILA; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE EXPRESSION REGULATION; GENE SILENCING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ANIMALS; ANTIGENS, CD8; CELL CYCLE; CELLS, CULTURED; CYCLIN E; DROSOPHILA; ENDORIBONUCLEASES; GENE SILENCING; HUMANS; LAC OPERON; RNA, DOUBLE-STRANDED; RNA, MESSENGER; SUBSTRATE SPECIFICITY; TRANSCRIPTION, GENETIC; TRANSFECTION;

CAENORHABDITIS ELEGANS; FUNGI; HYDRA; INVERTEBRATA; PLANARIA;

EID: 0034673638     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35005107     Document Type: Article

References (23)

1. Sharp, P. A. RNAi and double-strand RNA. Genes Dev. 13, 139-141 (1999).
2. Sanchez-Alvarado, A. & Newmark, P. A double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc. Natl Acad. Sci. USA 96, 5049-5054 (1999).
3. Lohmann, J. U., Endl, I. & Bosch, T. C. Silencing of developmental genes in Hydra. Dev. Biol. 214, 211-214 (1999).
4. Cogoni, C. & Macino, G. Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase. Nature 399, 166-169 (1999).
5. Waterhouse, P. M., Graham, M. W. & Wang, M. B. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl Acad. Sci. USA 95, 13959-13964 (1998).
6. Montgomery, M. K. & Fire, A. Double-stranded RNA as a mediator in sequence-specific genetic silencing and co-suppression. Trends Genet. 14, 225-228 (1998).
7. Ngo, H., I'schudi, C., Gull, K. & Ullu, E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucci. Proc. Natl Acad. Sci. USA 95, 14687-14692 (1998).
8. Tabara, H. et al. The rdc-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123-132 (1999).
9. Ketting, R. F., Haverkamp, T. H. A., van Luenen, H. G. A. M. & Plasterk, R. H. A. mut-7 of C. elegans, required tor transposon silencing and RNA interference, is a homolog of Werner Syndrome helicase and RnaseD. Cell 99, 133-141 (1999).
10. Ratcliff, F., Harrison, B. D. & Baulcombe, D. C. A similarity between viral defense and gene silencing in plants. Science 276, 1558-1560 (1997).
11. Kennerdell, J. R. & Carthew, R. W. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95, 1017-1026 (1998).
12. Misquitta, L. & Paterson, B. M. Targeted disruption of gene function in Drosophila by RNA interference: a role for nautilus in embryonic somatic muscle formation. Proc. Natl Acad. Sci. USA 96, 1451-1456 (1999).
13. Kaleita, R. F., Brideau, A. D., Banfield, B. W. & Beavis, A. J. An integral membrane green fluorescent protein market, Us9-GFP, is quantitatively retained in cells during propidium iodine-based cell cycle analysis by flow cytometry. Exp. Cell. Res. 248, 322-328 (1999).
14. Wolf, D. A. & Jackson, P. K. Cell cycle: oiling the gears of anaphase. Curr. Biol. 8, R637-R639 (1998).
15. Kramer, E. R., Gieffers, C., Holz, G., Hengstschlager, M. & Peters, J. M. Activation of the human anaphase-promoting complex by proteins of the CDC20/fizzy family. Curr. Biol. 8, 1207-1210 (1998).
16. Shuttleworth, J. & Colman, A. Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs. EMBO J. 7, 427-434 (1988).
17. Tabara, H., Grishok, A. & Mello, C. C. RNAi in C. elegans: soaking in the genome sequence. Science 282, 430-432 (1998).
18. Bosher, I. M., Dufourcq, P., Sookhareea, S. & Labouesse, M. RNA interference can target pre-mRNA. Consequences for gene expression in a Caenorhabditis elegans operon. Genetics 153, 1245-1256 (1999).
19. Hamilton, J. A. & Baulcombe, D. C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286, 950-952 (1999).
20. Jones, L. A., Thomas, C. L. & Maule, A. J. De novo methylation and co-suppression induced by a cytoplasmically replicating plant RNA virus. EMBO J. 17, 6385-6393 (1998).
21. Jones, L. A. et al. RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing. Plant Cell 11, 2291-2301 (1999).
22. Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morpho. 27, 353-365 (1972).
23. Di Nocera, P. P. & Dawid, I. B. Transient expression of genes introduced into cultured cells of Drosophila. Proc. Natl Acad. Sci. USA 80, 7095-7098 (1983).