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Volumn 9, Issue 7, 2008, Pages 554-566

The art and design of genetic screens: RNA interference

Author keywords

[No Author keywords available]

Indexed keywords

SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

EID: 45549089565     PISSN: 14710056     EISSN: 14710064     Source Type: Journal    
DOI: 10.1038/nrg2364     Document Type: Review
Times cited : (374)

References (97)
  • 1
    • 0032545933 scopus 로고    scopus 로고
    • Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    • This is the landmark paper that started the field of RNAi
    • Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811 (1998). This is the landmark paper that started the field of RNAi.
    • (1998) Nature , vol.391 , pp. 806-811
    • Fire, A.1
  • 2
    • 35348996554 scopus 로고    scopus 로고
    • Specialization and evolution of endogenous small RNA pathways
    • Chapman, E. J. & Carrington, J. C. Specialization and evolution of endogenous small RNA pathways. Nature Rev. Genet. 8, 884-896 (2007).
    • (2007) Nature Rev. Genet , vol.8 , pp. 884-896
    • Chapman, E.J.1    Carrington, J.C.2
  • 3
    • 3242677639 scopus 로고    scopus 로고
    • Dorsett, Y. & Tuschl, T. siRNAs: applications in functional genomics and potential as therapeutics. Nature Rev. Drug Discov. 3, 318-329 (2004).
    • Dorsett, Y. & Tuschl, T. siRNAs: applications in functional genomics and potential as therapeutics. Nature Rev. Drug Discov. 3, 318-329 (2004).
  • 4
    • 0037062951 scopus 로고    scopus 로고
    • RNA interference
    • Hannon, G. J. RNA interference. Nature 418, 244-251 (2002).
    • (2002) Nature , vol.418 , pp. 244-251
    • Hannon, G.J.1
  • 5
    • 23944506654 scopus 로고    scopus 로고
    • The love-hate relationship between Ras and Notch
    • Sundaram, M. V. The love-hate relationship between Ras and Notch. Genes Dev. 19, 1825-1839 (2005).
    • (2005) Genes Dev , vol.19 , pp. 1825-1839
    • Sundaram, M.V.1
  • 8
    • 0035657098 scopus 로고    scopus 로고
    • The art and design of genetic screens: Zebrafish
    • Patton, E. E. & Zon, L. I. The art and design of genetic screens: zebrafish. Nature Rev. Genet. 2, 956-966 (2001).
    • (2001) Nature Rev. Genet , vol.2 , pp. 956-966
    • Patton, E.E.1    Zon, L.I.2
  • 9
    • 1142274548 scopus 로고    scopus 로고
    • Identification of the gene for vitamin K epoxide reductase
    • This paper demonstrates the highly effective use of a directed RNAi screen
    • Li, T. et al. Identification of the gene for vitamin K epoxide reductase. Nature 427, 541-544 (2004). This paper demonstrates the highly effective use of a directed RNAi screen.
    • (2004) Nature , vol.427 , pp. 541-544
    • Li, T.1
  • 10
    • 34548295310 scopus 로고    scopus 로고
    • Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic
    • Balklava, Z., Pant, S., Fares, H. & Grant, B. D. Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic. Nature Cell Biol. 9, 1066-1073 (2007).
    • (2007) Nature Cell Biol , vol.9 , pp. 1066-1073
    • Balklava, Z.1    Pant, S.2    Fares, H.3    Grant, B.D.4
  • 11
    • 0842309871 scopus 로고    scopus 로고
    • Boutros, M. et al. Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303, 832-835 (2004). This paper details the first genome-scale RNAi screen in Drosophila cells.
    • Boutros, M. et al. Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303, 832-835 (2004). This paper details the first genome-scale RNAi screen in Drosophila cells.
  • 12
    • 34147198467 scopus 로고    scopus 로고
    • Synthetic lethal screen identification of chemosensitizer loci in cancer cells
    • Whitehurst, A. W. et al. Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Nature 446, 815-819 (2007).
    • (2007) Nature , vol.446 , pp. 815-819
    • Whitehurst, A.W.1
  • 13
    • 33646057010 scopus 로고    scopus 로고
    • High-throughput RNAi screening by time-lapse imaging of live human cells
    • This paper uses a combination of RNAi and time-lapse imaging, which proves to be a powerful approach that allows dynamic processes to be investigated
    • Neumann, B. et al. High-throughput RNAi screening by time-lapse imaging of live human cells. Nature Methods 3, 385-390 (2006). This paper uses a combination of RNAi and time-lapse imaging, which proves to be a powerful approach that allows dynamic processes to be investigated.
    • (2006) Nature Methods , vol.3 , pp. 385-390
    • Neumann, B.1
  • 14
    • 33749023011 scopus 로고    scopus 로고
    • Minimizing the risk of reporting false positives in large-scale RNAi screens
    • Echeverri, C. J. et al. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nature Methods 3, 777-779 (2006).
    • (2006) Nature Methods , vol.3 , pp. 777-779
    • Echeverri, C.J.1
  • 16
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • Zhang, J. H., Chung, T. D. & Oldenburg, K. R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen 4, 67-73 (1999).
    • (1999) J. Biomol. Screen , vol.4 , pp. 67-73
    • Zhang, J.H.1    Chung, T.D.2    Oldenburg, K.R.3
  • 17
    • 33749381471 scopus 로고    scopus 로고
    • Analysis of cell-based RNAi screens
    • Boutros, M., Bras., L. P. & Huber, W. Analysis of cell-based RNAi screens. Genome Biol. 7, R66 (2006).
    • (2006) Genome Biol , vol.7
    • Boutros, M.1    Bras, L.P.2    Huber, W.3
  • 18
    • 35848929692 scopus 로고    scopus 로고
    • A probability-based approach for the analysis of large-scale RNAi screens
    • Konig, R. et al. A probability-based approach for the analysis of large-scale RNAi screens. Nature Methods 4, 847-849 (2007).
    • (2007) Nature Methods , vol.4 , pp. 847-849
    • Konig, R.1
  • 19
    • 33845792555 scopus 로고    scopus 로고
    • CellProfiler: Image analysis software for identifying and quantifying cell phenotypes
    • Carpenter, A. E. et al. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol. 7, R100 (2006).
    • (2006) Genome Biol , vol.7
    • Carpenter, A.E.1
  • 20
    • 38549146881 scopus 로고    scopus 로고
    • WormBase 2007
    • Rogers, A. et al. WormBase 2007. Nucleic Acids Res. 36, D612-D617 (2008).
    • (2008) Nucleic Acids Res , vol.36
    • Rogers, A.1
  • 21
    • 0347755544 scopus 로고    scopus 로고
    • RNAiDB and PhenoBlast: Web tools for genome-wide phenotypic mapping projects
    • Gunsalus, K. C., Yueh, W. C., MacMenamin, P. & Piano, F. RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects. Nucleic Acids Res. 32, D406-410 (2004).
    • (2004) Nucleic Acids Res , vol.32
    • Gunsalus, K.C.1    Yueh, W.C.2    MacMenamin, P.3    Piano, F.4
  • 22
    • 33644876926 scopus 로고    scopus 로고
    • FLIGHT: Database and tools for the integration and cross-correlation of large-scale RNAi phenotypic datasets
    • Sims, D., Bursteinas, B., Gao, Q., Zvelebil, M. & Baum, B. FLIGHT: database and tools for the integration and cross-correlation of large-scale RNAi phenotypic datasets. Nucleic Acids Res. 34, D479-483 (2006).
    • (2006) Nucleic Acids Res , vol.34
    • Sims, D.1    Bursteinas, B.2    Gao, Q.3    Zvelebil, M.4    Baum, B.5
  • 23
  • 24
    • 33644873679 scopus 로고    scopus 로고
    • FlyRNAi: The Drosophila RNAi screening center database
    • Flockhart, I. et al. FlyRNAi: the Drosophila RNAi screening center database. Nucleic Acids Res. 34, D489-D494 (2006).
    • (2006) Nucleic Acids Res , vol.34
    • Flockhart, I.1
  • 25
    • 0032538391 scopus 로고    scopus 로고
    • RNAi in C. elegans: Soaking in the genome sequence
    • Tabara, H., Grishok, A. & Mello, C. C. RNAi in C. elegans: soaking in the genome sequence. Science 282, 430-431 (1998).
    • (1998) Science , vol.282 , pp. 430-431
    • Tabara, H.1    Grishok, A.2    Mello, C.C.3
  • 26
    • 0032578911 scopus 로고    scopus 로고
    • Specific interference by ingested dsRNA
    • Timmons, L. & Fire, A. Specific interference by ingested dsRNA. Nature 395, 854. (1998).
    • (1998) Nature , vol.395 , pp. 854
    • Timmons, L.1    Fire, A.2
  • 27
    • 0032717319 scopus 로고    scopus 로고
    • The rde-1 gene, RNA interference, and transposon silencing in C. elegans
    • Tabara, H. et al. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99, 123-132 (1999).
    • (1999) Cell , vol.99 , pp. 123-132
    • Tabara, H.1
  • 28
    • 0033968037 scopus 로고    scopus 로고
    • Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes
    • Tavernarakis, N., Wang, S. L., Dorovkov, M., Ryazanov, A. & Driscoll, M. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nature Genet. 24, 180-183 (2000).
    • (2000) Nature Genet , vol.24 , pp. 180-183
    • Tavernarakis, N.1    Wang, S.L.2    Dorovkov, M.3    Ryazanov, A.4    Driscoll, M.5
  • 29
    • 0035941485 scopus 로고    scopus 로고
    • Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
    • Timmons, L., Court, D. L. & Fire, A. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263, 103-112 (2001).
    • (2001) Gene , vol.263 , pp. 103-112
    • Timmons, L.1    Court, D.L.2    Fire, A.3
  • 30
    • 0034676448 scopus 로고    scopus 로고
    • Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
    • Gonczy, P. et al. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331-336 (2000).
    • (2000) Nature , vol.408 , pp. 331-336
    • Gonczy, P.1
  • 31
    • 0035128859 scopus 로고    scopus 로고
    • Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi
    • Maeda, I., Kohara, Y., Yamamoto, M. & Sugimoto, A. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11, 171-176 (2001).
    • (2001) Curr. Biol , vol.11 , pp. 171-176
    • Maeda, I.1    Kohara, Y.2    Yamamoto, M.3    Sugimoto, A.4
  • 32
    • 15844431376 scopus 로고    scopus 로고
    • Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
    • Sonnichsen, B. et al. Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature 434, 462-469 (2005).
    • (2005) Nature , vol.434 , pp. 462-469
    • Sonnichsen, B.1
  • 33
    • 0034676457 scopus 로고    scopus 로고
    • Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
    • Fraser, A. G. et al. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325-330 (2000).
    • (2000) Nature , vol.408 , pp. 325-330
    • Fraser, A.G.1
  • 34
    • 0037448540 scopus 로고    scopus 로고
    • Kamath, R. S. et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237 (2003). References 33 and 34 describe the first genome-scale RNAi screen in C. elegans, which was also the first in any animal.
    • Kamath, R. S. et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237 (2003). References 33 and 34 describe the first genome-scale RNAi screen in C. elegans, which was also the first in any animal.
  • 35
    • 26444458292 scopus 로고    scopus 로고
    • Functional genomic analysis of C. elegans molting
    • Frand, A. R., Russel, S. & Ruvkun, G. Functional genomic analysis of C. elegans molting. PLoS Biol. 3, e312 (2005).
    • (2005) PLoS Biol , vol.3
    • Frand, A.R.1    Russel, S.2    Ruvkun, G.3
  • 36
    • 33745648779 scopus 로고    scopus 로고
    • Galphao/i and Galphas signaling function in parallel with the MSP/Eph receptor to control meiotic diapause in C. elegans
    • Govindan, J. A., Cheng, H., Harris, J. E. & Greenstein, D. Galphao/i and Galphas signaling function in parallel with the MSP/Eph receptor to control meiotic diapause in C. elegans. Curr. Biol. 16, 1257-1268 (2006).
    • (2006) Curr. Biol , vol.16 , pp. 1257-1268
    • Govindan, J.A.1    Cheng, H.2    Harris, J.E.3    Greenstein, D.4
  • 37
    • 0037443054 scopus 로고    scopus 로고
    • Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi
    • Pothof, J. et al. Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi. Genes Dev. 17, 443-448 (2003).
    • (2003) Genes Dev , vol.17 , pp. 443-448
    • Pothof, J.1
  • 38
    • 34247556560 scopus 로고    scopus 로고
    • Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway
    • Longman, D., Plasterk, R. H., Johnstone, I. L. & Caceres, J. F. Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway. Genes Dev. 21, 1075-1085 (2007).
    • (2007) Genes Dev , vol.21 , pp. 1075-1085
    • Longman, D.1    Plasterk, R.H.2    Johnstone, I.L.3    Caceres, J.F.4
  • 39
    • 33748908963 scopus 로고    scopus 로고
    • A genomewide screen for suppressors of par-2 uncovers potential regulators of PAR protein-dependent cell polarity in Caenorhabditis elegans
    • Labbe, J. C., Pacquelet, A., Marty, T. & Gotta, M. A genomewide screen for suppressors of par-2 uncovers potential regulators of PAR protein-dependent cell polarity in Caenorhabditis elegans. Genetics 174, 285-295 (2006).
    • (2006) Genetics , vol.174 , pp. 285-295
    • Labbe, J.C.1    Pacquelet, A.2    Marty, T.3    Gotta, M.4
  • 40
    • 34548387960 scopus 로고    scopus 로고
    • Dynein modifiers in C. elegans: Light chains suppress conditional heavy chain mutants
    • O'Rourke, S. M., Dorfman, M. D., Carter, J. C. & Bowerman, B. Dynein modifiers in C. elegans: light chains suppress conditional heavy chain mutants. PLoS Genet. 3, e128 (2007).
    • (2007) PLoS Genet , vol.3
    • O'Rourke, S.M.1    Dorfman, M.D.2    Carter, J.C.3    Bowerman, B.4
  • 41
    • 0031767247 scopus 로고    scopus 로고
    • Genetics of RAS signaling in C. elegans
    • Sternberg, P. W. & Han, M. Genetics of RAS signaling in C. elegans. Trends Genet. 14, 466-472 (1998).
    • (1998) Trends Genet , vol.14 , pp. 466-472
    • Sternberg, P.W.1    Han, M.2
  • 42
    • 23044477079 scopus 로고    scopus 로고
    • Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans
    • Poulin, G., Dong, Y., Fraser, A. G., Hopper, N. A. & Ahringer, J. Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans. EMBO J. 24, 2613-2623 (2005).
    • (2005) EMBO J , vol.24 , pp. 2613-2623
    • Poulin, G.1    Dong, Y.2    Fraser, A.G.3    Hopper, N.A.4    Ahringer, J.5
  • 43
    • 33646501892 scopus 로고    scopus 로고
    • Cui, M., Kim, E. B. & Han, M. Diverse chromatin remodeling genes antagonize the Rb-involved SynMuv pathways in C. elegans. PLoS Genet. 2, e74 (2006).
    • Cui, M., Kim, E. B. & Han, M. Diverse chromatin remodeling genes antagonize the Rb-involved SynMuv pathways in C. elegans. PLoS Genet. 2, e74 (2006).
  • 44
    • 33746525415 scopus 로고    scopus 로고
    • Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways
    • Lehner, B., Crombie, C., Tischler, J., Fortunato, A. & Fraser, A. G. Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways. Nature Genet. 38, 896-903 (2006).
    • (2006) Nature Genet , vol.38 , pp. 896-903
    • Lehner, B.1    Crombie, C.2    Tischler, J.3    Fortunato, A.4    Fraser, A.G.5
  • 45
    • 34848834757 scopus 로고    scopus 로고
    • Byrne, A. B. et al. A global analysis of genetic interactions in Caenorhabditis elegans. J. Biol. 6, 8 (2007). References 44 and 45 use RNAi to explore genetic interactions on a large scale, a powerful approach that is likely to be increasingly used in the future.
    • Byrne, A. B. et al. A global analysis of genetic interactions in Caenorhabditis elegans. J. Biol. 6, 8 (2007). References 44 and 45 use RNAi to explore genetic interactions on a large scale, a powerful approach that is likely to be increasingly used in the future.
  • 46
    • 33646127607 scopus 로고    scopus 로고
    • FGF negatively regulates muscle membrane extension in Caenorhabditis elegans
    • Dixon, S. J., Alexander, M., Fernandes, R., Ricker, N. & Roy, P. J. FGF negatively regulates muscle membrane extension in Caenorhabditis elegans. Development 133, 1263-1275 (2006).
    • (2006) Development , vol.133 , pp. 1263-1275
    • Dixon, S.J.1    Alexander, M.2    Fernandes, R.3    Ricker, N.4    Roy, P.J.5
  • 47
    • 33749182338 scopus 로고    scopus 로고
    • Oocyte signals derived from polyunsaturated fatty acids control sperm recruitment in vivo
    • Kubagawa, H. M. et al. Oocyte signals derived from polyunsaturated fatty acids control sperm recruitment in vivo. Nature Cell Biol. 8, 1143-1148 (2006).
    • (2006) Nature Cell Biol , vol.8 , pp. 1143-1148
    • Kubagawa, H.M.1
  • 48
    • 36549001859 scopus 로고    scopus 로고
    • Parry, D. H., Xu, J. & Ruvkun, G. A whole-genome RNAi screen for C. elegans miRNA pathway genes. Curr. Biol. (2007).
    • Parry, D. H., Xu, J. & Ruvkun, G. A whole-genome RNAi screen for C. elegans miRNA pathway genes. Curr. Biol. (2007).
  • 49
    • 33750124210 scopus 로고    scopus 로고
    • Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system
    • Pulak, R. Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system. Methods Mol. Biol. 351, 275-286 (2006).
    • (2006) Methods Mol. Biol , vol.351 , pp. 275-286
    • Pulak, R.1
  • 50
    • 0034612276 scopus 로고    scopus 로고
    • Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
    • Clemens, J. C. et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl Acad. Sci. USA 97, 6499-6503 (2000).
    • (2000) Proc. Natl Acad. Sci. USA , vol.97 , pp. 6499-6503
    • Clemens, J.C.1
  • 51
    • 85088687112 scopus 로고    scopus 로고
    • Drosophila cell lines as model systems and as an experimental tool
    • in the press
    • Baum, B. & Cherbas, L. Drosophila cell lines as model systems and as an experimental tool. Methods Mol. Biol. (in the press).
    • Methods Mol. Biol
    • Baum, B.1    Cherbas, L.2
  • 52
    • 33646123992 scopus 로고    scopus 로고
    • Secretion of Wnt ligands requires Evi, a conserved transmembrane protein
    • Bartscherer, K., Pelte, N., Ingelfinger, D. & Boutros, M. Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 125, 523-533 (2006).
    • (2006) Cell , vol.125 , pp. 523-533
    • Bartscherer, K.1    Pelte, N.2    Ingelfinger, D.3    Boutros, M.4
  • 53
    • 18244400410 scopus 로고    scopus 로고
    • Functional genomic analysis of the Wnt-wingless signaling pathway
    • DasGupta, R., Kaykas, A., Moon, R. T. & Perrimon, N. Functional genomic analysis of the Wnt-wingless signaling pathway. Science 308, 826-833 (2005).
    • (2005) Science , vol.308 , pp. 826-833
    • DasGupta, R.1    Kaykas, A.2    Moon, R.T.3    Perrimon, N.4
  • 54
    • 27144475536 scopus 로고    scopus 로고
    • Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway
    • Kleino, A. et al. Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway. EMBO J. 24, 3423-3434 (2005).
    • (2005) EMBO J , vol.24 , pp. 3423-3434
    • Kleino, A.1
  • 55
    • 23844483215 scopus 로고    scopus 로고
    • Identification of JAK/STAT signalling components by genome-wide RNA interference
    • Muller, P., Kuttenkeuler, D., Gesellchen, V., Zeidler, M. P. & Boutros, M. Identification of JAK/STAT signalling components by genome-wide RNA interference. Nature 436, 871-875 (2005).
    • (2005) Nature , vol.436 , pp. 871-875
    • Muller, P.1    Kuttenkeuler, D.2    Gesellchen, V.3    Zeidler, M.P.4    Boutros, M.5
  • 56
    • 38449084517 scopus 로고    scopus 로고
    • Design and implementation of high-throughput RNAi screens in cultured Drosophila cells
    • Ramadan, N., Flockhart, I., Booker, M., Perrimon, N. & Mathey-Prevot, B. Design and implementation of high-throughput RNAi screens in cultured Drosophila cells. Nature Protoc. 2, 2245-2264 (2007).
    • (2007) Nature Protoc , vol.2 , pp. 2245-2264
    • Ramadan, N.1    Flockhart, I.2    Booker, M.3    Perrimon, N.4    Mathey-Prevot, B.5
  • 57
    • 33645732587 scopus 로고    scopus 로고
    • Internalization is required for proper Wingless signaling in Drosophila melanogaster
    • Seto, E. S. & Bellen, H. J. Internalization is required for proper Wingless signaling in Drosophila melanogaster. J. Cell Biol. 173, 95-106 (2006).
    • (2006) J. Cell Biol , vol.173 , pp. 95-106
    • Seto, E.S.1    Bellen, H.J.2
  • 58
    • 33750887331 scopus 로고    scopus 로고
    • A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling
    • Friedman, A. & Perrimon, N. A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Nature 444, 230-234 (2006).
    • (2006) Nature , vol.444 , pp. 230-234
    • Friedman, A.1    Perrimon, N.2
  • 59
    • 33744479684 scopus 로고    scopus 로고
    • 2+ entry
    • 2+ entry. Science 312, 1220-1223 (2006).
    • (2006) Science , vol.312 , pp. 1220-1223
    • Vig, M.1
  • 60
    • 0033883114 scopus 로고    scopus 로고
    • Heritable gene silencing in Drosophila using double-stranded RNA
    • Kennerdell, J. R. & Carthew, R. W. Heritable gene silencing in Drosophila using double-stranded RNA. Nature Biotechnol. 18, 896-898 (2000).
    • (2000) Nature Biotechnol , vol.18 , pp. 896-898
    • Kennerdell, J.R.1    Carthew, R.W.2
  • 61
    • 34248400087 scopus 로고    scopus 로고
    • RNA interference screen to identify genes required for Drosophila embryonic nervous system development
    • Koizumi, K. et al. RNA interference screen to identify genes required for Drosophila embryonic nervous system development. Proc. Natl Acad. Sci. USA 104, 5626-5631 (2007).
    • (2007) Proc. Natl Acad. Sci. USA , vol.104 , pp. 5626-5631
    • Koizumi, K.1
  • 62
    • 33644993688 scopus 로고    scopus 로고
    • Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation
    • Pilot, F., Philippe, J. M., Lemmers, C., Chauvin, J. P. & Lecuit, T. Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation. Development 133, 711-723 (2006).
    • (2006) Development , vol.133 , pp. 711-723
    • Pilot, F.1    Philippe, J.M.2    Lemmers, C.3    Chauvin, J.P.4    Lecuit, T.5
  • 63
    • 34447530305 scopus 로고    scopus 로고
    • Dietzl, G. et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151-156 (2007). This paper and NIG-FLY describe the generation of libraries of transgenic Drosophila to allow whole-animal RNAi screens.
    • Dietzl, G. et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151-156 (2007). This paper and NIG-FLY describe the generation of libraries of transgenic Drosophila to allow whole-animal RNAi screens.
  • 64
    • 36448942505 scopus 로고    scopus 로고
    • SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth
    • This paper shows that primary Drosophila cells can be used for RNAi screening, paving the way for more diverse screens in the future
    • Bai, J., Hartwig, J. H. & Perrimon, N. SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth. Dev. Cell 13, 828-842 (2007). This paper shows that primary Drosophila cells can be used for RNAi screening, paving the way for more diverse screens in the future.
    • (2007) Dev. Cell , vol.13 , pp. 828-842
    • Bai, J.1    Hartwig, J.H.2    Perrimon, N.3
  • 65
    • 44449117370 scopus 로고    scopus 로고
    • RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance
    • Bai, J. et al. RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Development 135, 1439-1449 (2008).
    • (2008) Development , vol.135 , pp. 1439-1449
    • Bai, J.1
  • 66
    • 33847660443 scopus 로고    scopus 로고
    • An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases
    • Bischof, J., Maeda, R. K., Hediger, M., Karch, F. & Basler, K. An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc. Natl Acad. Sci. USA 104, 3312-3317 (2007).
    • (2007) Proc. Natl Acad. Sci. USA , vol.104 , pp. 3312-3317
    • Bischof, J.1    Maeda, R.K.2    Hediger, M.3    Karch, F.4    Basler, K.5
  • 67
    • 37749006118 scopus 로고    scopus 로고
    • Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
    • Ni, J. Q. et al. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nature Methods 5, 49-51 (2008).
    • (2008) Nature Methods , vol.5 , pp. 49-51
    • Ni, J.Q.1
  • 68
    • 33748939584 scopus 로고    scopus 로고
    • Prevalence of off-target effects in Drosophila RNA interference screens
    • Ma, Y., Creanga, A., Lum, L. & Beachy, P. A. Prevalence of off-target effects in Drosophila RNA interference screens. Nature 443, 359-363 (2006).
    • (2006) Nature , vol.443 , pp. 359-363
    • Ma, Y.1    Creanga, A.2    Lum, L.3    Beachy, P.A.4
  • 69
    • 33748994797 scopus 로고    scopus 로고
    • Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays
    • Kulkarni, M. M. et al. Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nature Methods 3, 833-838 (2006).
    • (2006) Nature Methods , vol.3 , pp. 833-838
    • Kulkarni, M.M.1
  • 70
    • 23144441141 scopus 로고    scopus 로고
    • Arziman, Z., Horn, T. & Boutros, M. E-RNAi: a web application to design optimized RNAi constructs. Nucleic Acids Res. 33, W582-588 (2005).
    • Arziman, Z., Horn, T. & Boutros, M. E-RNAi: a web application to design optimized RNAi constructs. Nucleic Acids Res. 33, W582-588 (2005).
  • 71
    • 33646187810 scopus 로고    scopus 로고
    • High-throughput RNAi screening in cultured cells: A user's guide
    • Echeverri, C. J. & Perrimon, N. High-throughput RNAi screening in cultured cells: a user's guide. Nature Rev. Genet. 7, 373-384 (2006).
    • (2006) Nature Rev. Genet , vol.7 , pp. 373-384
    • Echeverri, C.J.1    Perrimon, N.2
  • 72
    • 0035942736 scopus 로고    scopus 로고
    • Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    • Elbashir, S. M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498 (2001).
    • (2001) Nature , vol.411 , pp. 494-498
    • Elbashir, S.M.1
  • 73
    • 12144289681 scopus 로고    scopus 로고
    • A large-scale RNAi screen in human cells identifies new components of the p53 pathway
    • Berns, K. et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428, 431-437 (2004).
    • (2004) Nature , vol.428 , pp. 431-437
    • Berns, K.1
  • 74
    • 12144288006 scopus 로고    scopus 로고
    • Paddison, P. J. et al. A resource for large-scale RNA-interference-based screens in mammals. Nature 428, 427-431 (2004). References 73 and 74 describe genome-scale RNAi screening in human cells.
    • Paddison, P. J. et al. A resource for large-scale RNA-interference-based screens in mammals. Nature 428, 427-431 (2004). References 73 and 74 describe genome-scale RNAi screening in human cells.
  • 75
    • 38849192515 scopus 로고    scopus 로고
    • Profiling essential genes in human mammary cells by multiplex RNAi screening
    • Silva, J. M. et al. Profiling essential genes in human mammary cells by multiplex RNAi screening. Science 319, 617-620 (2008).
    • (2008) Science , vol.319 , pp. 617-620
    • Silva, J.M.1
  • 76
    • 38849098442 scopus 로고    scopus 로고
    • Cancer proliferation gene discovery through functional genomics
    • Schlabach, M. R. et al. Cancer proliferation gene discovery through functional genomics. Science 319, 620-624 (2008).
    • (2008) Science , vol.319 , pp. 620-624
    • Schlabach, M.R.1
  • 77
    • 33646033137 scopus 로고    scopus 로고
    • A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen
    • Moffat, J. et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124, 1283-1298 (2006).
    • (2006) Cell , vol.124 , pp. 1283-1298
    • Moffat, J.1
  • 78
    • 36749016685 scopus 로고    scopus 로고
    • Genome-scale RNAi profiling of cell division in human tissue culture cells
    • Kittler, R. et al. Genome-scale RNAi profiling of cell division in human tissue culture cells. Nature Cell Biol. 9, 1401-1412 (2007).
    • (2007) Nature Cell Biol , vol.9 , pp. 1401-1412
    • Kittler, R.1
  • 79
    • 35148885729 scopus 로고    scopus 로고
    • A functional genetic approach identifies the PI3K pathway as a major determinant of trastuzumab resistance in breast cancer
    • Berns, K. et al. A functional genetic approach identifies the PI3K pathway as a major determinant of trastuzumab resistance in breast cancer. Cancer Cell 12, 395-402 (2007).
    • (2007) Cancer Cell , vol.12 , pp. 395-402
    • Berns, K.1
  • 80
    • 33748209616 scopus 로고    scopus 로고
    • Efficient prediction of siRNAs with siRNArules 1.0: An open-source JAVA approach to siRNA algorithms
    • Holen, T. Efficient prediction of siRNAs with siRNArules 1.0: an open-source JAVA approach to siRNA algorithms. RNA 12, 1620-1625 (2006).
    • (2006) RNA , vol.12 , pp. 1620-1625
    • Holen, T.1
  • 81
    • 33746772726 scopus 로고    scopus 로고
    • Demonstration of two novel methods for predicting functional siRNA efficiency
    • Jia, P., Shi, T., Cai, Y. & Li, Y. Demonstration of two novel methods for predicting functional siRNA efficiency. BMC Bioinformatics 7, 271 (2006).
    • (2006) BMC Bioinformatics , vol.7 , pp. 271
    • Jia, P.1    Shi, T.2    Cai, Y.3    Li, Y.4
  • 82
    • 33846634136 scopus 로고    scopus 로고
    • Selecting effective siRNAs based on guide RNA structure
    • Koberle, C., Kaufmann, S. H. & Patzel, V. Selecting effective siRNAs based on guide RNA structure. Nature Protoc. 1, 1832-1839 (2006).
    • (2006) Nature Protoc , vol.1 , pp. 1832-1839
    • Koberle, C.1    Kaufmann, S.H.2    Patzel, V.3
  • 83
    • 0033214763 scopus 로고    scopus 로고
    • Silencing of developmental genes in Hydra
    • Lohmann, J. U., Endl, I. & Bosch, T. C. Silencing of developmental genes in Hydra. Dev. Biol. 214, 211-214 (1999).
    • (1999) Dev. Biol , vol.214 , pp. 211-214
    • Lohmann, J.U.1    Endl, I.2    Bosch, T.C.3
  • 84
    • 0033608994 scopus 로고    scopus 로고
    • Double-stranded RNA specifically disrupts gene expression during planarian regeneration
    • Sanchez Alvarado, A. & Newmark, P. A. Double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc. Natl Acad. Sci. USA 96, 5049-5054 (1999).
    • (1999) Proc. Natl Acad. Sci. USA , vol.96 , pp. 5049-5054
    • Sanchez Alvarado, A.1    Newmark, P.A.2
  • 85
    • 17844395204 scopus 로고    scopus 로고
    • Identification of genes needed for regeneration, stem cell function, and tissue homeostasis by systematic gene perturbation in planaria
    • Reddien, P. W., Bermange, A. L., Murfitt, K. J., Jennings, J. R. & Sánchez Alvarado, A. Identification of genes needed for regeneration, stem cell function, and tissue homeostasis by systematic gene perturbation in planaria. Dev. Cell 8, 635-649 (2005).
    • (2005) Dev. Cell , vol.8 , pp. 635-649
    • Reddien, P.W.1    Bermange, A.L.2    Murfitt, K.J.3    Jennings, J.R.4    Sánchez Alvarado, A.5
  • 86
    • 21844450491 scopus 로고    scopus 로고
    • Functional genomic analysis of midgut epithelial responses in Anopheles during Plasmodium invasion
    • Vlachou, D., Schlegelmilch, T., Christophides, G. K. & Kafatos, F. C. Functional genomic analysis of midgut epithelial responses in Anopheles during Plasmodium invasion. Curr. Biol. 15, 1185-1195 (2005).
    • (2005) Curr. Biol , vol.15 , pp. 1185-1195
    • Vlachou, D.1    Schlegelmilch, T.2    Christophides, G.K.3    Kafatos, F.C.4
  • 87
    • 33646826325 scopus 로고    scopus 로고
    • Toward silencing the burden of malaria: Progress and prospects for RNAi-based approaches
    • Brown, A. E. & Catteruccia, F. Toward silencing the burden of malaria: progress and prospects for RNAi-based approaches. Biotechniques Suppl, 38-44 (2006).
    • (2006) Biotechniques , Issue.SUPPL. , pp. 38-44
    • Brown, A.E.1    Catteruccia, F.2
  • 88
    • 33751067329 scopus 로고    scopus 로고
    • A method for parental RNA interference in the wasp Nasonia vitripennis
    • Lynch, J. A. & Desplan, C. A method for parental RNA interference in the wasp Nasonia vitripennis. Nature Protoc 1, 486-494 (2006).
    • (2006) Nature Protoc , vol.1 , pp. 486-494
    • Lynch, J.A.1    Desplan, C.2
  • 89
    • 0033634682 scopus 로고    scopus 로고
    • Functional anatomy of a dsRNA trigger: Differential requirement for the two trigger strands in RNA interference
    • Parrish, S., Fleenor, J., Xu, S., Mello, C. & Fire, A. Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference. Mol. Cell 6, 1077-1087 (2000).
    • (2000) Mol. Cell , vol.6 , pp. 1077-1087
    • Parrish, S.1    Fleenor, J.2    Xu, S.3    Mello, C.4    Fire, A.5
  • 90
    • 7444270371 scopus 로고    scopus 로고
    • Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library
    • Rual, J. F. et al. Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 14, 2162-2168 (2004).
    • (2004) Genome Res , vol.14 , pp. 2162-2168
    • Rual, J.F.1
  • 91
    • 45549087241 scopus 로고    scopus 로고
    • Ahringer, J. (ed.) Reverse Genetics, Wormbook [online], 〈http://www.wormbook.org/chapters/www_introreversegenetics/ introreversegenetics.html〉 (ed. The C. elegans Research Community, 2006).
    • Ahringer, J. (ed.) Reverse Genetics, Wormbook [online], 〈http://www.wormbook.org/chapters/www_introreversegenetics/ introreversegenetics.html〉 (ed. The C. elegans Research Community, 2006).
  • 92
    • 34249740079 scopus 로고    scopus 로고
    • RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions
    • Lehner, B., Tischler, J. & Fraser, A. G. RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions. Nature Protoc. 1, 1617-1620 (2006).
    • (2006) Nature Protoc , vol.1 , pp. 1617-1620
    • Lehner, B.1    Tischler, J.2    Fraser, A.G.3
  • 93
    • 1342346710 scopus 로고    scopus 로고
    • A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
    • Kennedy, S., Wang, D. & Ruvkun, G. A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427, 645-649 (2004).
    • (2004) Nature , vol.427 , pp. 645-649
    • Kennedy, S.1    Wang, D.2    Ruvkun, G.3
  • 94
    • 33846503468 scopus 로고    scopus 로고
    • Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive Caenorhabditis elegans strain nre-1(hd20) lin-15b(hd126)
    • Schmitz, C., Kinge, P. & Hutter, H. Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive Caenorhabditis elegans strain nre-1(hd20) lin-15b(hd126). Proc. Natl Acad. Sci. USA 104, 834-839 (2007).
    • (2007) Proc. Natl Acad. Sci. USA , vol.104 , pp. 834-839
    • Schmitz, C.1    Kinge, P.2    Hutter, H.3
  • 95
    • 23144441321 scopus 로고    scopus 로고
    • Systematic analysis of genes required for synapse structure and function
    • Sieburth, D. et al. Systematic analysis of genes required for synapse structure and function. Nature 436, 510-517 (2005).
    • (2005) Nature , vol.436 , pp. 510-517
    • Sieburth, D.1
  • 96
    • 0037031152 scopus 로고    scopus 로고
    • Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi
    • Simmer, F. et al. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr. Biol. 12, 1317-1319 (2002).
    • (2002) Curr. Biol , vol.12 , pp. 1317-1319
    • Simmer, F.1
  • 97
    • 33745614074 scopus 로고    scopus 로고
    • Position-specific chemical modification of siRNAs reduces 'off-target' transcript silencing
    • Jackson, A. L. et al. Position-specific chemical modification of siRNAs reduces 'off-target' transcript silencing. RNA 12, 1197-1205 (2006).
    • (2006) RNA , vol.12 , pp. 1197-1205
    • Jackson, A.L.1


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