A novel miRNA processing pathway independent of dicer requires argonaute2 catalytic activity

Science

Volumn 328, Issue 5986, 2010, Pages 1694-1698

  Cifuentes, Daniel ^a   Xue, Huiling ^a   Taylor, David W ^a   Patnode, Heather ^a   Mishima, Yukhiro ^a,b   Cheloufi, Sihem ^c,d   Ma, Enbo ^e   Mane, Shrikant ^a   Hannon, Gregory J ^c   Lawson, Nathan D ^f   Wolfe, Scot A ^f,g   Giraldez, Antonio J ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b KOBE UNIVERSITY   (Japan)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d STONY BROOK UNIVERSITY   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f UNIVERSITY OF MASSACHUSETTS   (United States)

^g UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGONAUTE 2 PROTEIN; DICER; MESSENGER RNA;

BIOCHEMISTRY; CATALYSIS; CYTOLOGY; ENZYME ACTIVITY; MUTATION; RNA;

ARTICLE; BIOGENESIS; CATALYSIS; CONTROLLED STUDY; ERYTHROPOIESIS; GENE MUTATION; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; RNA PROCESSING; ZEBRA FISH;

ANIMALS; BIOCATALYSIS; EMBRYO, NONMAMMALIAN; EMBRYONIC DEVELOPMENT; ERYTHROPOIESIS; EUKARYOTIC INITIATION FACTOR-2; HUMANS; MICRORNAS; MODELS, BIOLOGICAL; MORPHOGENESIS; NUCLEIC ACID CONFORMATION; RECOMBINANT PROTEINS; RIBONUCLEASE III; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 77953897182     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1190809     Document Type: Article

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31. We thank J. Doudna, D. O'Carroll, L. Zon, S. Lacadie, G. Lieschke, D. Krausse, and S. Halene for reagents and protocols; A. Enright, C. Abreu-Goodger, and J. Brennecke for initial small RNA analysis; and B. Schachter, C. Takacs, V. Greco, and D. CazaHa for discussions and manuscript editing. Supported by Fundación Ramdn Areces (D.C.), a Human Frontier Science Program fellowship (H.X.), NIH grants R01GM081602-03/03S1 (A.J.G.) and R01HL093766 (N.D.L. and S.A.W.), the Yale Scholar program, and the Pew Scholars Program in the Biomedical Sciences (A.J.G.). Contributions; D.C. and A.J.G. designed and performed experiments; H.X. performed computational analysis; D.C. and D.W.T. performed in vitro assays; H.P. performed in situ hybridizations; Y.M., G.J.H., and S.C. helped with initial smalt RNA library sequencing and discussion; E.M. provided recombinant Ago2; S.M. provided small RNA sequencing; SAW. and N.L designed the zinc finger nucleases; and A.J.G. wrote the manuscript. Sequencing data are deposited in Gene Expression Omnibus (accession number GSE21503).