-
1
-
-
33646857665
-
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
-
Arnau J., et al. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr. Purif. 2006, 48:1-13.
-
(2006)
Protein Expr. Purif.
, vol.48
, pp. 1-13
-
-
Arnau, J.1
-
2
-
-
15744372778
-
Comparison of affinity tags for protein purification
-
Lichty J.J., et al. Comparison of affinity tags for protein purification. Protein Expr. Purif. 2005, 41:98-105.
-
(2005)
Protein Expr. Purif.
, vol.41
, pp. 98-105
-
-
Lichty, J.J.1
-
3
-
-
0037255597
-
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
-
Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 2003, 60:523-533.
-
(2003)
Appl. Microbiol. Biotechnol.
, vol.60
, pp. 523-533
-
-
Terpe, K.1
-
4
-
-
19444373996
-
Making the most of affinity tags
-
Waugh D.S. Making the most of affinity tags. Trends Biotechnol. 2005, 23:316-320.
-
(2005)
Trends Biotechnol.
, vol.23
, pp. 316-320
-
-
Waugh, D.S.1
-
5
-
-
0036421180
-
Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag
-
Humphries H.E., et al. Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag. Protein Expr. Purif. 2002, 26:243-248.
-
(2002)
Protein Expr. Purif.
, vol.26
, pp. 243-248
-
-
Humphries, H.E.1
-
6
-
-
23944463921
-
Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system
-
Sharma S.S., et al. Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system. Appl. Biochem. Biotechnol. 2005, 126:93-118.
-
(2005)
Appl. Biochem. Biotechnol.
, vol.126
, pp. 93-118
-
-
Sharma, S.S.1
-
7
-
-
0038648648
-
Crystal structures of fusion proteins with large-affinity tags
-
Smyth D.R., et al. Crystal structures of fusion proteins with large-affinity tags. Protein Sci. 2003, 12:1313-1322.
-
(2003)
Protein Sci.
, vol.12
, pp. 1313-1322
-
-
Smyth, D.R.1
-
8
-
-
0032757296
-
Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose
-
Chaga G., et al. Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose. J. Chromatogr. A 1999, 864:247-256.
-
(1999)
J. Chromatogr. A
, vol.864
, pp. 247-256
-
-
Chaga, G.1
-
9
-
-
0033814033
-
Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins
-
Skerra A., Schmidt T.G. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. Methods Enzymol. 2000, 326:271-304.
-
(2000)
Methods Enzymol.
, vol.326
, pp. 271-304
-
-
Skerra, A.1
Schmidt, T.G.2
-
10
-
-
23844438834
-
Self-cleavable stimulus responsive tags for protein purification without chromatography
-
Ge X., et al. Self-cleavable stimulus responsive tags for protein purification without chromatography. J. Am. Chem. Soc. 2005, 127:11228-11229.
-
(2005)
J. Am. Chem. Soc.
, vol.127
, pp. 11228-11229
-
-
Ge, X.1
-
11
-
-
24044491571
-
Simple bioseparations using self-cleaving elastin-like polypeptide tags
-
Banki M.R., et al. Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat. Methods 2005, 2:659-661.
-
(2005)
Nat. Methods
, vol.2
, pp. 659-661
-
-
Banki, M.R.1
-
12
-
-
0032739304
-
Purification of recombinant proteins by fusion with thermally-responsive polypeptides
-
Meyer D.E., Chilkoti A. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat. Biotechnol. 1999, 17:1112-1115.
-
(1999)
Nat. Biotechnol.
, vol.17
, pp. 1112-1115
-
-
Meyer, D.E.1
Chilkoti, A.2
-
13
-
-
34548841392
-
Recombinant protein purification by self-cleaving aggregation tag
-
Wu W.Y., et al. Recombinant protein purification by self-cleaving aggregation tag. Nat. Protoc. 2006, 1:2257-2262.
-
(2006)
Nat. Protoc.
, vol.1
, pp. 2257-2262
-
-
Wu, W.Y.1
-
14
-
-
2542628131
-
Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides
-
Meyer D.E., Chilkoti A. Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. Biomacromolecules 2004, 5:846-851.
-
(2004)
Biomacromolecules
, vol.5
, pp. 846-851
-
-
Meyer, D.E.1
Chilkoti, A.2
-
15
-
-
0023953406
-
Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics
-
Urry D.W. Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics. J. Protein Chem. 1988, 7:1-34.
-
(1988)
J. Protein Chem.
, vol.7
, pp. 1-34
-
-
Urry, D.W.1
-
16
-
-
0038388786
-
Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers
-
Urry D.W. Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers. J. Phys. Chem. B 1997, 101:11007-11028.
-
(1997)
J. Phys. Chem. B
, vol.101
, pp. 11007-11028
-
-
Urry, D.W.1
-
17
-
-
0026926763
-
Hydrophobicity scale for proteins based on inverse temperature transitions
-
Urry D.W., et al. Hydrophobicity scale for proteins based on inverse temperature transitions. Biopolymers 1992, 32:1243-1250.
-
(1992)
Biopolymers
, vol.32
, pp. 1243-1250
-
-
Urry, D.W.1
-
18
-
-
28144460981
-
Inteins and affinity resin substitutes for protein purification and scale up
-
Banki M.R., Wood D.W. Inteins and affinity resin substitutes for protein purification and scale up. Microb. Cell Fact. 2005, 4:32.
-
(2005)
Microb. Cell Fact.
, vol.4
, pp. 32
-
-
Banki, M.R.1
Wood, D.W.2
-
19
-
-
9344234999
-
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion
-
Trabbic-Carlson K., et al. Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein Sci. 2004, 13:3274-3284.
-
(2004)
Protein Sci.
, vol.13
, pp. 3274-3284
-
-
Trabbic-Carlson, K.1
-
20
-
-
65549096684
-
Optimization of elastin-like polypeptide fusions for expression and purification of recombinant proteins in plants
-
Conley A.J., et al. Optimization of elastin-like polypeptide fusions for expression and purification of recombinant proteins in plants. Biotechnol. Bioeng. 2009, 103:562-573.
-
(2009)
Biotechnol. Bioeng.
, vol.103
, pp. 562-573
-
-
Conley, A.J.1
-
21
-
-
33748747042
-
Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cycling
-
Lin M., et al. Functional expression of a biologically active fragment of soluble gp130 as an ELP-fusion protein in transgenic plants: purification via inverse transition cycling. Biochem. J. 2006, 398:577-583.
-
(2006)
Biochem. J.
, vol.398
, pp. 577-583
-
-
Lin, M.1
-
22
-
-
22444444258
-
Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association
-
Banki M.R., et al. Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein Sci. 2005, 14:1387-1395.
-
(2005)
Protein Sci.
, vol.14
, pp. 1387-1395
-
-
Banki, M.R.1
-
23
-
-
26844545316
-
Integrated recombinant protein expression and purification platform based on Ralstonia eutropha
-
Barnard G.C., et al. Integrated recombinant protein expression and purification platform based on Ralstonia eutropha. Appl. Environ. Microbiol. 2005, 71:5735-5742.
-
(2005)
Appl. Environ. Microbiol.
, vol.71
, pp. 5735-5742
-
-
Barnard, G.C.1
-
24
-
-
2942558481
-
In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF
-
Moldes C., et al. In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF. Appl. Environ. Microbiol. 2004, 70:3205-3212.
-
(2004)
Appl. Environ. Microbiol.
, vol.70
, pp. 3205-3212
-
-
Moldes, C.1
-
25
-
-
54349115910
-
A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles
-
Wang Z., et al. A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles. Lab Chip 2008, 8:1957-1962.
-
(2008)
Lab Chip
, vol.8
, pp. 1957-1962
-
-
Wang, Z.1
-
26
-
-
35649017202
-
A novel, cheap and effective fusion expression system for the production of recombinant proteins
-
Ding F.X., et al. A novel, cheap and effective fusion expression system for the production of recombinant proteins. Appl. Microbiol. Biotechnol. 2007, 77:483-488.
-
(2007)
Appl. Microbiol. Biotechnol.
, vol.77
, pp. 483-488
-
-
Ding, F.X.1
-
27
-
-
1442338396
-
Non-fusion expression in Escherichia coli, purification, and characterization of a novel Ca2+- and phospholipid-binding protein annexin B1
-
Zhang Y., et al. Non-fusion expression in Escherichia coli, purification, and characterization of a novel Ca2+- and phospholipid-binding protein annexin B1. Protein Expr. Purif. 2004, 34:68-74.
-
(2004)
Protein Expr. Purif.
, vol.34
, pp. 68-74
-
-
Zhang, Y.1
-
28
-
-
34249903605
-
Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides
-
Lim D.W., et al. Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules 2007, 8:1417-1424.
-
(2007)
Biomacromolecules
, vol.8
, pp. 1417-1424
-
-
Lim, D.W.1
-
29
-
-
0028214350
-
Protein splicing elements: inteins and exteins-a definition of terms and recommended nomenclature
-
Perler F.B., et al. Protein splicing elements: inteins and exteins-a definition of terms and recommended nomenclature. Nucleic Acids Res. 1994, 22:1125-1127.
-
(1994)
Nucleic Acids Res.
, vol.22
, pp. 1125-1127
-
-
Perler, F.B.1
-
30
-
-
0032534048
-
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
-
Chong S., et al. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 1998, 26:5109-5115.
-
(1998)
Nucleic Acids Res.
, vol.26
, pp. 5109-5115
-
-
Chong, S.1
-
31
-
-
0036405356
-
Inteins: structure, function, and evolution
-
Gogarten J.P., et al. Inteins: structure, function, and evolution. Annu. Rev. Microbiol. 2002, 56:263-287.
-
(2002)
Annu. Rev. Microbiol.
, vol.56
, pp. 263-287
-
-
Gogarten, J.P.1
-
32
-
-
0029834656
-
Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system
-
Chong S., et al. Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J. Biol. Chem. 1996, 271:22159-22168.
-
(1996)
J. Biol. Chem.
, vol.271
, pp. 22159-22168
-
-
Chong, S.1
-
33
-
-
0026462996
-
Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing
-
Hirata R., Anraku Y. Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing. Biochem. Biophys. Res. Commun. 1992, 188:40-47.
-
(1992)
Biochem. Biophys. Res. Commun.
, vol.188
, pp. 40-47
-
-
Hirata, R.1
Anraku, Y.2
-
34
-
-
0033614484
-
Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation
-
Mathys S., et al. Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 1999, 231:1-13.
-
(1999)
Gene
, vol.231
, pp. 1-13
-
-
Mathys, S.1
-
35
-
-
0034518359
-
Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor
-
Wood D.W., et al. Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor. Biotechnol. Prog. 2000, 16:1055-1063.
-
(2000)
Biotechnol. Prog.
, vol.16
, pp. 1055-1063
-
-
Wood, D.W.1
-
36
-
-
0032832776
-
A genetic system yields self-cleaving inteins for bioseparations
-
Wood D.W., et al. A genetic system yields self-cleaving inteins for bioseparations. Nat. Biotechnol. 1999, 17:889-892.
-
(1999)
Nat. Biotechnol.
, vol.17
, pp. 889-892
-
-
Wood, D.W.1
-
37
-
-
0027984269
-
Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation
-
Xu M.Q., et al. Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. EMBO J. 1994, 13:5517-5522.
-
(1994)
EMBO J.
, vol.13
, pp. 5517-5522
-
-
Xu, M.Q.1
-
38
-
-
47749156371
-
Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system
-
Esipov R.S., et al. Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system. Protein Expr. Purif. 2008, 61:1-6.
-
(2008)
Protein Expr. Purif.
, vol.61
, pp. 1-6
-
-
Esipov, R.S.1
-
39
-
-
33745991799
-
Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli
-
Sharma S.S., et al. Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli. J. Biotechnol. 2006, 125:48-56.
-
(2006)
J. Biotechnol.
, vol.125
, pp. 48-56
-
-
Sharma, S.S.1
-
40
-
-
37449032617
-
Purification of green fluorescent protein using a two-intein system
-
Zhao Z., et al. Purification of green fluorescent protein using a two-intein system. Appl. Microbiol. Biotechnol. 2008, 77:1175-1180.
-
(2008)
Appl. Microbiol. Biotechnol.
, vol.77
, pp. 1175-1180
-
-
Zhao, Z.1
-
41
-
-
44349158280
-
Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage
-
Hong J., et al. Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. J. Chromatogr. A 2008, 1194:150-154.
-
(2008)
J. Chromatogr. A
, vol.1194
, pp. 150-154
-
-
Hong, J.1
-
42
-
-
51649125516
-
Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags
-
Gillies A.R., et al. Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags. Biotechnol. Bioeng. 2008, 101:229-240.
-
(2008)
Biotechnol. Bioeng.
, vol.101
, pp. 229-240
-
-
Gillies, A.R.1
-
43
-
-
17744415288
-
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
-
Chong S., et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 1997, 192:271-281.
-
(1997)
Gene
, vol.192
, pp. 271-281
-
-
Chong, S.1
-
44
-
-
0034965749
-
Intein-mediated rapid purification of Cre recombinase
-
Cantor E.J., Chong S. Intein-mediated rapid purification of Cre recombinase. Protein Expr. Purif. 2001, 22:135-140.
-
(2001)
Protein Expr. Purif.
, vol.22
, pp. 135-140
-
-
Cantor, E.J.1
Chong, S.2
-
45
-
-
0035913755
-
Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
-
Zhang A., et al. Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 2001, 275:241-252.
-
(2001)
Gene
, vol.275
, pp. 241-252
-
-
Zhang, A.1
-
46
-
-
37349023278
-
Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris
-
Babu K., et al. Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris. Protein Expr. Purif. 2008, 57:201-205.
-
(2008)
Protein Expr. Purif.
, vol.57
, pp. 201-205
-
-
Babu, K.1
-
47
-
-
2942620151
-
A novel " clip-and-link" activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond
-
Osicka R., et al. A novel " clip-and-link" activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond. J. Biol. Chem. 2004, 279:24944-24956.
-
(2004)
J. Biol. Chem.
, vol.279
, pp. 24944-24956
-
-
Osicka, R.1
-
48
-
-
52949085497
-
Single-step affinity purification of recombinant proteins using a self-excising module from Neisseria meningitidis FrpC
-
Sadilkova L., et al. Single-step affinity purification of recombinant proteins using a self-excising module from Neisseria meningitidis FrpC. Protein Sci. 2008, 17:1834-1843.
-
(2008)
Protein Sci.
, vol.17
, pp. 1834-1843
-
-
Sadilkova, L.1
-
49
-
-
0033618622
-
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
-
Mazmanian S.K., et al. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science 1999, 285:760-763.
-
(1999)
Science
, vol.285
, pp. 760-763
-
-
Mazmanian, S.K.1
-
50
-
-
1542317850
-
Sortase-mediated protein ligation: a new method for protein engineering
-
Mao H., et al. Sortase-mediated protein ligation: a new method for protein engineering. J. Am. Chem. Soc. 2004, 126:2670-2671.
-
(2004)
J. Am. Chem. Soc.
, vol.126
, pp. 2670-2671
-
-
Mao, H.1
-
51
-
-
3543063548
-
A self-cleavable sortase fusion for one-step purification of free recombinant proteins
-
Mao H. A self-cleavable sortase fusion for one-step purification of free recombinant proteins. Protein Expr. Purif. 2004, 37:253-263.
-
(2004)
Protein Expr. Purif.
, vol.37
, pp. 253-263
-
-
Mao, H.1
-
52
-
-
1042288291
-
Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA
-
Kruger R.G., et al. Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA. Biochemistry 2004, 43:1541-1551.
-
(2004)
Biochemistry
, vol.43
, pp. 1541-1551
-
-
Kruger, R.G.1
-
53
-
-
34648828739
-
Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags
-
Arnau J., et al. Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags. Nat. Protoc. 2006, 1:2326-2333.
-
(2006)
Nat. Protoc.
, vol.1
, pp. 2326-2333
-
-
Arnau, J.1
-
54
-
-
0033117468
-
Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases
-
Pedersen J., et al. Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Protein Expr. Purif. 1999, 15:389-400.
-
(1999)
Protein Expr. Purif.
, vol.15
, pp. 389-400
-
-
Pedersen, J.1
-
55
-
-
77951978302
-
A Process for Producing Human Growth Hormone
-
European Patent EP0217814, filed 02/06/1986, publication date 12/21/1994
-
Andersen, H., Dalboge, et al. (1994) A Process for Producing Human Growth Hormone. European Patent EP0217814, filed 02/06/1986, publication date 12/21/1994.
-
(1994)
-
-
Andersen, H.1
Dalboge2
-
56
-
-
0029740605
-
Human insulin: basic sciences to therapeutic uses
-
Chien Y.W. Human insulin: basic sciences to therapeutic uses. Drug Dev. Ind. Pharm. 1996, 22:753-789.
-
(1996)
Drug Dev. Ind. Pharm.
, vol.22
, pp. 753-789
-
-
Chien, Y.W.1
-
57
-
-
0032988320
-
Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein
-
Southworth M.W., et al. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 1999, 27:110-120.
-
(1999)
Biotechniques
, vol.27
, pp. 110-120
-
-
Southworth, M.W.1
-
58
-
-
0037157179
-
Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system
-
Morassutti C., et al. Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system. FEBS Lett. 2002, 519:141-146.
-
(2002)
FEBS Lett.
, vol.519
, pp. 141-146
-
-
Morassutti, C.1
-
59
-
-
0032722062
-
Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation
-
Pradhan S., et al. Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation. J. Biol. Chem. 1999, 274:33002-33010.
-
(1999)
J. Biol. Chem.
, vol.274
, pp. 33002-33010
-
-
Pradhan, S.1
-
60
-
-
0032122306
-
Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems
-
Verma R., et al. Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J. Immunol. Methods 1998, 216:165-181.
-
(1998)
J. Immunol. Methods
, vol.216
, pp. 165-181
-
-
Verma, R.1
-
61
-
-
0021991801
-
The synthesis and in vivo assembly of functional antibodies in yeast
-
Wood C.R., et al. The synthesis and in vivo assembly of functional antibodies in yeast. Nature 1985, 314:446-449.
-
(1985)
Nature
, vol.314
, pp. 446-449
-
-
Wood, C.R.1
-
62
-
-
0029392471
-
Production of human insulin in an E. coli system with Met-Lys-human proinsulin as the expressed precursor
-
Chen J.Q., et al. Production of human insulin in an E. coli system with Met-Lys-human proinsulin as the expressed precursor. Appl. Biochem. Biotechnol. 1995, 55:5-15.
-
(1995)
Appl. Biochem. Biotechnol.
, vol.55
, pp. 5-15
-
-
Chen, J.Q.1
-
63
-
-
34248202284
-
Intein-mediated expression is an effective approach in the study of beta-defensins
-
Diao H., et al. Intein-mediated expression is an effective approach in the study of beta-defensins. Biochem. Biophys. Res. Commun. 2007, 357:840-846.
-
(2007)
Biochem. Biophys. Res. Commun.
, vol.357
, pp. 840-846
-
-
Diao, H.1
-
64
-
-
67349157562
-
Optimization of ELP-intein mediated protein purification by salt substitution
-
Fong B.A., et al. Optimization of ELP-intein mediated protein purification by salt substitution. Protein Expr. Purif. 2009, 66:198-202.
-
(2009)
Protein Expr. Purif.
, vol.66
, pp. 198-202
-
-
Fong, B.A.1
|