Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

Protein Science

Volumn 13, Issue 12, 2004, Pages 3274-3284

  Trabbic Carlson, Kimberly ^a   Liu, Li ^b   Kim, Bumjoon ^a   Chilkoti, Ashutosh ^a  

^a Duke University   (United States)

^b Neose Technologies Inc   (United States)

Author keywords

Elastin; Elastin like polypeptide; Fusion protein; Phase transition; Protein purification; Solubility tag

Indexed keywords

BLUE FLUORESCENT PROTEIN; CALMODULIN; CHLORAMPHENICOL ACETYLTRANSFERASE; ELASTIN; HISTIDINE; HYBRID PROTEIN; RECOMBINANT PROTEIN; THIOREDOXIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BATCH PROCESS; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PHASE TRANSITION; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOMICS; SCALE UP; SOLUBILITY;

ELASTIN; ESCHERICHIA COLI; HISTIDINE; PEPTIDES; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS; NEGIBACTERIA;

EID: 9344234999     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.04931604     Document Type: Article

References (23)

1. Betre, H., Setton, L.A., Meyer, D.E., and Chilkoti, A. 2002. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules 3: 910-916.
2. Grossman, T.H., Kawasaki, E.S., Punreddy, S.R., and Osburne, M.S. 1998. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209: 95-103.
3. Guda, C., Zhang, X., McPherson, D.T., Xu, J., Cherry, J.H., Urry, D.W., and Daniell, H. 1995. Hyper expression of an environmentally friendly synthetic polymer gene. Biotechnol. Lett. 17: 745-750.
4. Lavallie, E.R., Diblasio, E.A., Kovacic, S., Grant, K.L., Schendel, P.F., and McCoy, J.M. 1993. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia-coli cytoplasm. Biotechnology 11: 187-193.
5. Li, B. and Daggett, V. 2003. The molecular basis of the temperature- and pH-induced conformational transitions in elastin-based peptides. Biopolymers 68: 121-129.
6. Li, B., Alonso, O.V., and Daggett, V. 2001. The molecular basis for the inverse temperature transition of elastin. J. Mol. Biol. 305: 581-592.
7. Maina, C.V., Riggs, P.D., Grandea, A.G.I., Slatko, B.E., Moran, L.S., Tagliamonte, J.A., McReynolds, L.A., and diGuan, C. 1988. An Escherichia coli vector to express and purify foreign proteins by fusion 10 and separation from maltose binding protein. Gene 74: 365-373.
8. Meyer, D.E. and Chilkoti, A. 1999. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat. Biotechnol. 17: 1112-1115.
9. -. 2002. Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: Examples from the elastin-like polypeptide system. Biomacromolecules 3: 357-367.
10. Meyer, D.E., Trabbic-Carlson, K., and Chilkoti, A. 2001. Protein purification by fusion with an environmentally responsive elastin-like polypeptide: Effect of polypeptide length on the purification of thioredoxin. Biotechnol. Prog. 17: 720-728.
11. Nilsson, J., Ståhl, S., Lundeberg, J., Uhlén, M., and Nygren, P.Å. 1997. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr. Purif. 11: 1-16.
12. Sassenfeld, H.M. 1990. Engineering proteins for purification. Trends Biotechnol. 8: 88-93.
13. Shimoboji, T., Larenas, E., Fowler, T., Kulkarni, S., Huffman, A.S., and Stayton, P.S. 2002. Photoresponsive polymer-enzyme switches. Proc. Natl. Acad Sci. 99: 16592-16596.
14. Shimoboji, T., Larenas, E., Fowler, T., Hoffman, A.S., and Stayton, P.S. 2003. Temperature-induced switching of enzyme activity with smart polymer-enzyme conjugates. Bioconjug. Chem. 14: 517-525.
15. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40.
16. Trabbic-Carlson, K., Setton, L.A., and Chilkoti, A. 2003. Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules 4: 572-580.
17. Trabbic-Carlson, K., Meyer, D.E., Liu, L., Piervincenzi, R., Nath, N., LaBean, T., and Chilkoti, A. 2004. Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: A role for surface hydrophobicity? Protein Eng. Des. Sel. 17: 57-66.
18. Uhlén, M. and Moks, T. 1990. Gene fusions for purpose of expression: An introduction. Methods Enzymol. 195: 129-143.
19. Urry, D.W. 1988. Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics. J. Protein Chem. 7: 1-34.
20. - 1992. Free energy transduction in polypeptides and proteins based on inverse temperature transitions. Prog. Biophys. Mol. Biol. 57: 23-57.
21. -. 1997. Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers. J. Phys. Chem. 101: 11007-11028.
22. Urry, D.W., Trapane, T.L., and Prasad, K.U. 1985. Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies. Biopolymers 24: 2345-2356.
23. Walker, S.W., Wark, J.D., Macneil, S., Mellersh, H., Brown, B.L., and Tomlinson, S. 1984. Isolation, purification and cell-free synthesis of calmodulin from the pig anterior-pituitary gland. Biochem. J. 217: 827-832.