Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli

Journal of Biotechnology

Volumn 125, Issue 1, 2006, Pages 48-56

  Sharma, Shamik S ^a,b   Chong, Shaorong ^b   Harcum, Sarah W ^c  

^a South   (United States)

^b NEW ENGLAND BIOLABS   (United States)

^c CLEMSON UNIVERSITY   (United States)

Author keywords

Antitrypsin; Chitin; Cre recombinase; Fusion protein; Human bFGF; Intein; Recombinant

Indexed keywords

CELLS; CHITIN; ESCHERICHIA COLI; PH EFFECTS; PURIFICATION; SUBSTRATES;

ANTITRYPSIN; CRE RECOMBINASE; FUSION PROTEIN; HUMAN BFGF; INTEIN;

PROTEINS;

CHITIN; HYBRID PROTEIN; INTEIN;

ARTICLE; CATALYSIS; CELL CULTURE EQUIPMENT; CELL DENSITY; ESCHERICHIA COLI; FED BATCH FERMENTATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; TEMPERATURE;

ALPHA 1-ANTITRYPSIN; CELL DIVISION; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTORS; HUMANS; INTEGRASES; INTEINS; PROTEIN SPLICING; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 33745991799     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.01.018     Document Type: Article

References (50)

1. Anspach F.B., Curbelo D., Hartmann R., Garke G., and Deckwer W.-D. Expanded-bed chromatography in primary protein purification. J. Chromatogr. A 865 (1999) 129-144
2. Barker A.F., Siemsen F., Pasley D., D'Silva R., and Buist A.S. Replacement therapy for hereditary alpha 1-antitrypsin deficiency: a program for long term administration. Chest 105 (1994) 1406-1410
3. Bylund F., Castan A., Mikkola R., Veide A., and Larsson G. Influence of scale-up on the quality of recombinant human growth hormone. Biotechnol. Bioeng. 69 (2000) 119-128
4. Cantor E.J., and Chong S.R. Intein-mediated rapid purification of Cre recombinase. Protein Expr. Purif. 22 (2001) 135-140
5. Chong S.R., Mersha F.B., Comb D.G., Scott M.E., Landry D., Vence L.M., Perler F.B., Benner J., Kucera R.B., Hirvonen C.A., Pelletier J.J., Paulus H., and Xu M.Q. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192 (1997) 271-281
6. Chong S.R., Montello G.E., Zhang A.H., Cantor E.J., Liao W., Xu M.Q., and Benner J. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26 (1998) 5109-5115
7. Chong S.R., Shao Y., Paulus H., Benner J., Perler F.B., and Xu M.Q. Protein splicing involving the Saccharomyces cerevisiae VMA intein-the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J. Biol. Chem. 271 (1996) 22159-22168
8. Chong S.R., Williams K.S., Wotkowicz C., and Xu M.Q. Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J. Biol. Chem. 273 (1998) 10567-10577
9. Crowe J., Dobeli H., Gentz R., Hochuli E., Stuber D., and Henco K. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol. 31 (1994) 371-387
10. DeLisa M.P., Li J., Rao G., Weigand W.A., and Bentley W.E. Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using an on-line optical sensor. Biotechnol. Bioeng. 65 (1999) 54-64
11. Derbyshire V., and Belfort M. Lightning strikes twice: intron-intein coincidence. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 1356-1357
12. Derbyshire V., Wood D.W., Wu W., Dansereau J.T., Dalgaard J.Z., and Belfort M. Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution. Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 11466-11471
13. 1-antitrypsin deficiency. Chest 122 (2002) 1818-1829
14. DiGuan C., Li P., Riggs P.D., and Inouye H. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67 (1988) 21-30
15. Enfors S.O., Jahic M., Rozkov A., Xu B., Hecker M., Jurgen B., Kruger E., Schweder T., Hamer G., O'Beirne D., Noisommit-Rizzi N., Reuss M., Boone L., Hewitt C., McFarlane C., Nienow A., Kovacs T., Tragardh C., Fuchs L., Revstedt J., Friberg P.C., Hjertager B., Blomsten G., Skogman H., Hjort S., Hoeks F., Lin H.Y., Neubauer P., van der Lans R., Luyben K., Vrabel P., and Manelius A. Physiological responses to mixing in large scale bioreactors. J. Biotechnol. 85 (2001) 175-185
16. Gill R.T., Delisa M.P., Valdes J.J., and Bentley W.E. Genomic analysis of high cell density recombinant Escherichia coli fermentation and "cell conditioning" for improved recombinant protein yield. Biotechnol. Bioeng. 72 (2001) 85-95
17. Gill R.T., Valdes J.J., and Bentley W.E. A comparative study of global stress gene regulation in response to overexpression of recombinant protein in Escherichia coli. Met. Eng. 2 (2000) 178-189
18. Gorman C., and Bullock C. Site-specific gene targeting for gene expression in eukaryotes. Curr. Opin. Biotechnol. 11 (2000) 455-460
19. Guo C.Y., Li Z.Y., Shi Y.W., Xu M.Q., Wise J.G., Trommer W.E., and Yuan J.M. Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Protein Expr. Purif. 37 (2004) 361-367
20. Haddadin F.T., and Harcum S.W. Transcriptome profiles for high-cell-density recombinant and wild type Escherichia coli. Biotechnol. Bioeng. 90 (2005) 127-153
21. Hsu C., and Chang J. Clinical implications of growth factors in flexor tendon wound healing. J. Hand Surg. 29A (2004) 551-563
22. ™, CN Instruction Manual, New England Biolabs, 2004
23. +-adenosine triphosphatase. Science 250 (1990) 651-657
24. Korz D.J., Rinas U., Hellmuth K., Sanders E.A., and Deckwer W.D. Simple fed-batch technique for high cell-density cultivation of Escherichia coli. J. Biotechnol. 39 (1995) 59-65
25. LaVallie E.R., and McCoy J.M. Gene fusion expression systems in Escherichia coli. Curr. Opin. Biotechnol. (1995) 501-506
26. Lee S.Y. High cell-density culture of Escherichia coli. Trends Biotechnol. 14 (1996) 98-105
27. Ling T.C., Lyddiatt A., Carmichael I., Purdom G., Hathi P., and Levison P.R. Direct enzyme adsorption from an unclarified microbial feedstock using suspended bed chromatography. J. Chromatogr. A 989 (2003) 109-118
28. Liu Q.H., Li M.Z., Liu D., and Elledge S.J. Rapid construction of recombinant DNA by the univector plasmid-fusion system. Methods Enzymol. 328 (2000) 530-549
29. Lyddiatt A. Process chromatography: current constraints and future options for the absorptive recovery of bioproducts. Curr. Opin. Biotechnol. 13 (2002) 95-103
30. 1-antitrypsin. Biotechnol. Prog. 20 (2004) 269-276
31. Mathys S., Evans T.C., Chute I.C., Wu H., Chong S.R., Benner J., Liu X.Q., and Xu M.Q. Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231 (1999) 1-13
32. Metzger D., and Feil R. Engineering the mouse genome by site-specific recombination. Curr. Opin. Biotechnol. 10 (1999) 470-476
33. Oh M.K., and Liao J.C. Gene expression profiling by DNA microarrays and metabolic fluxes in Escherichia coli. Biotechnol. Prog. 16 (2000) 278-286
34. Paulus H. Protein splicing and related forms of protein autoprocessing. Annu. Rev. Biochem. 69 (2000) 447-496
35. Perler F.B., and Adam E. Protein splicing and its applications. Curr. Opin. Biotechnol. 11 (2000) 377-383
36. Perler F.B., Davis E.O., Dean G.E., Gimble F.S., Jack W.E., Neff N., Noren C.J., Thorner J., and Belfort M. Protein splicing elements - inteins and exteins - a definition of terms and recommended nomenclature. Nucleic Acids Res. 22 (1994) 1125-1127
37. Perler F.B., Olsen G.J., and Adam E. Compilation and analysis of intein sequences. Nucleic Acids Res. 25 (1997) 1087-1093
38. Scheinowitz M. Therapeutic myocardial angiogenesis: past, present and future. Mol. Cell. Biochem. 264 (2004) 75-83
39. Schuler M., and Kargi F. Bioprocess Engineering-Basic Concepts (1992), Prentice Hall, PTR, New Jersey
40. Smith D.B., and Johnson K.S. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67 (1988) 31-40
41. Southworth M., Amaya K., Evans T., Xu M., and Perler F. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27 (1999) 110-120
42. 1-antitrypsin. Enzyme Microb. Technol. 29 (2001) 611-620
43. Wood D. Simplified protein purification using engineered self-cleaving affinity tags. J. Chem. Technol. Biotechnol. 78 (2003) 103-110
44. Wood D.W., Wu W., Belfort G., Derbyshire V., and Belfort M. A genetic system yields self-cleaving inteins for bioseparations. Nat. Biotechnol. 17 (1999) 889-892
45. Wu W., Wood D.W., Belfort G., Derbyshire V., and Belfort M. Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing. Nucleic Acids Res. 30 (2002) 4864-4871
46. Xu M.Q., Paulus H., and Chong S.R. Fusions to self-splicing inteins for protein purification. Appl. Chimeric Genes Hybrid Proteins, Pt A 326 (2000) 376-418
47. Xu M.Q., and Perler F.B. The mechanism of protein splicing and its modulation by mutation. EMBO J. 15 (1996) 5146-5153
48. Xu M.Q., Southworth M.W., Mersha F.B., Hornstra L.J., and Perler F.B. In-vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 75 (1993) 1371-1377
49. Yoon S.H., Han M.-J., Lee S.Y., Jeong K.J., and Yoo J.-S. Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture. Biotechnol. Bioeng. 81 (2003) 753-767
50. Zhang A.H., Gonzalez S.M., Cantor E.J., and Chong S.R. Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275 (2001) 241-252