Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins

Gene

Volumn 275, Issue 2, 2001, Pages 241-252

  Zhang, Aihua ^a   Gonzalez, Sandra M ^b   Cantor, Eric J ^a   Chong, Shaorong ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b UNIVERSIDAD AUTÓNOMA DE CHIHUAHUA   (Mexico)

Author keywords

Cleavage; Green fluorescent protein; Protein splicing

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INTEIN; INTEIN PHO POIII; INTEIN PHO RADA; INTEIN SSP DNAE; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CODON; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

BASE SEQUENCE; CLONING, MOLECULAR; DNA; DNA POLYMERASE III; GENE EXPRESSION REGULATION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SPLICING; PROTEINS; RECOMBINANT FUSION PROTEINS; SULFHYDRYL COMPOUNDS;

ESCHERICHIA COLI;

EID: 0035913755     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00663-1     Document Type: Article

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