-
1
-
-
17744415288
-
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
-
Chong, S., F.B. Mersha, D.G. Comb, M.E. Scott, D. Landry, L.M. Vence, F.B. Perler, J. Banner, R.B. Kucera, C.A. Hirvonen, J.J. Pelletier, H. Paulus and M.Q. Xu. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192:271-281.
-
(1997)
Gene
, vol.192
, pp. 271-281
-
-
Chong, S.1
Mersha, F.B.2
Comb, D.G.3
Scott, M.E.4
Landry, D.5
Vence, L.M.6
Perler, F.B.7
Banner, J.8
Kucera, R.B.9
Hirvonen, C.A.10
Pelletier, J.J.11
Paulus, H.12
Xu, M.Q.13
-
2
-
-
0032534048
-
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
-
Chong, S., G.E. Montello, A. Zhang, E.J. Cantor, W. Liao, M.Q. Xu and J. Benner. 1998. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:5109-5115.
-
(1998)
Nucleic Acids Res.
, vol.26
, pp. 5109-5115
-
-
Chong, S.1
Montello, G.E.2
Zhang, A.3
Cantor, E.J.4
Liao, W.5
Xu, M.Q.6
Benner, J.7
-
3
-
-
0029834656
-
Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system
-
Chong, S., Y. Shao, H. Paulus, J. Benner, F.B. Perler and M.Q. Xu. 1996. Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J. Biol. Chem. 271:22159-22168.
-
(1996)
J. Biol. Chem.
, vol.271
, pp. 22159-22168
-
-
Chong, S.1
Shao, Y.2
Paulus, H.3
Benner, J.4
Perler, F.B.5
Xu, M.Q.6
-
4
-
-
0032562685
-
Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
-
Chong, S., K.S. Williams, C. Wotkowicz and M.Q. Xu. 1998. Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J. Biol. Chem. 273:10567-10577.
-
(1998)
J. Biol. Chem.
, vol.273
, pp. 10567-10577
-
-
Chong, S.1
Williams, K.S.2
Wotkowicz, C.3
Xu, M.Q.4
-
5
-
-
0344351815
-
Semisynthesis of cytotoxic proteins using a modified protein splicing element
-
Evans, T.C., J. Benner and M.-Q. Xu. 1998. Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7:2256-2264.
-
(1998)
Protein Sci.
, vol.7
, pp. 2256-2264
-
-
Evans, T.C.1
Benner, J.2
Xu, M.-Q.3
-
6
-
-
0032193937
-
Putting protein splicing to work
-
Gimble, F.S. 1998. Putting protein splicing to work. Chem. Biol. 5:R251-R256.
-
(1998)
Chem. Biol.
, vol.5
-
-
Gimble, F.S.1
-
7
-
-
0028825817
-
Gene fusion expression systems in Escherichia coli
-
LaVallie, E.R. and J.M. McCoy. 1995. Gene fusion expression systems in Escherichia coli. Curr. Opin. Biotechnol. 6:501-506.
-
(1995)
Curr. Opin. Biotechnol.
, vol.6
, pp. 501-506
-
-
LaVallie, E.R.1
McCoy, J.M.2
-
8
-
-
0032499752
-
Expressed protein ligation: A general method for protein engineering
-
Muir, T.W., D. Sondhi and P.A. Cole. 1998. Expressed protein ligation: a general method for protein engineering. Proc. Natl. Acad. Sci. USA 95:6705-6710.
-
(1998)
Proc. Natl. Acad. Sci. USA
, vol.95
, pp. 6705-6710
-
-
Muir, T.W.1
Sondhi, D.2
Cole, P.A.3
-
9
-
-
0032894598
-
InBase: The New England Biolabs intein database
-
Perler, F.B. 1999. InBase: the New England Biolabs intein database. Nucleic Acids Res. 27:346-347.
-
(1999)
Nucleic Acids Res.
, vol.27
, pp. 346-347
-
-
Perler, F.B.1
-
11
-
-
0032481347
-
Control of protein splicing by intein fragment reassembly
-
Southworth, M.W., E. Adam, D. Panne, R. Byer, R. Kautz and F.B. Perler. 1998. Control of protein splicing by intein fragment reassembly. EMBO J. 17:918-926.
-
(1998)
EMBO J.
, vol.17
, pp. 918-926
-
-
Southworth, M.W.1
Adam, E.2
Panne, D.3
Byer, R.4
Kautz, R.5
Perler, F.B.6
-
12
-
-
0030761813
-
The Mycobacterium xenopi GyrA protein splicing element: Characterization of a minimal intein
-
Telenti, A., M. Southworth, F. Alcaide, S. Daugelat, W.R. Jacobs, Jr. and F.B. Perler. 1997. The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein. J. Bacteriol. 179:6378-6382.
-
(1997)
J. Bacteriol.
, vol.179
, pp. 6378-6382
-
-
Telenti, A.1
Southworth, M.2
Alcaide, F.3
Daugelat, S.4
Jacobs Jr., W.R.5
Perler, F.B.6
-
13
-
-
0027984269
-
Protein splicing: An analysis of the branched intermediate and its resolution by succinimide formation
-
Xu, M., D.G. Comb, H. Paulus, C.J. Noren, Y. Shao and F.B. Perler. 1994. Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. EMBO J. 13:5517-5522.
-
(1994)
EMBO J.
, vol.13
, pp. 5517-5522
-
-
Xu, M.1
Comb, D.G.2
Paulus, H.3
Noren, C.J.4
Shao, Y.5
Perler, F.B.6
-
14
-
-
0029838842
-
The mechanism of protein splicing and its modulation by mutation
-
Xu, M. and F.B. Perler. 1996. The mechanism of protein splicing and its modulation by mutation. EMBO J. 15:5146-5153.
-
(1996)
EMBO J.
, vol.15
, pp. 5146-5153
-
-
Xu, M.1
Perler, F.B.2
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