Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor

Biotechnology Progress

Volumn 16, Issue 6, 2000, Pages 1055-1063

  Wood, D W ^a   Derbyshire, V ^a   Wu, W ^a   Chartrain, M ^a   Belfort, M ^a   Belfort, G ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIBROBLAST GROWTH FACTOR; HYBRID PROTEIN; INTEIN; PROTEIN DOMAIN; PROTEIN PURIFICATION; PROTEIN TRANSPORT;

CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBROBLAST GROWTH FACTOR 1; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MODELS, CHEMICAL; TEMPERATURE;

EID: 0034518359     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0000858     Document Type: Article

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