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Volumn 1, Issue 5, 2006, Pages 2326-2333

Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags

Author keywords

[No Author keywords available]

Indexed keywords

DIPEPTIDYL PEPTIDASE I; HISTIDINE; INTERLEUKIN 1BETA; POLYHISTIDINE; RECOMBINANT PROTEIN;

EID: 34648828739     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.388     Document Type: Article
Times cited : (26)

References (9)
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    • Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
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    • (2006) Protein Expr. Purif , vol.48 , pp. 1-13
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  • 2
    • 0033117468 scopus 로고    scopus 로고
    • Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases
    • Pedersen, J., Lauritzen, C., Madsen, M.T. & Weis Dahl, S. Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Protein Expr. Purif. 15, 389-400 (1999).
    • (1999) Protein Expr. Purif , vol.15 , pp. 389-400
    • Pedersen, J.1    Lauritzen, C.2    Madsen, M.T.3    Weis Dahl, S.4
  • 3
    • 31044446735 scopus 로고    scopus 로고
    • Rapid and easy thermodynamic optimization of the 5′-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli
    • Cebe, R. & Geiser, M. Rapid and easy thermodynamic optimization of the 5′-end of mRNA dramatically increases the level of wild type protein expression in Escherichia coli. Protein Expr. Purif. 45, 374-380 (2006).
    • (2006) Protein Expr. Purif , vol.45 , pp. 374-380
    • Cebe, R.1    Geiser, M.2
  • 4
    • 33646882130 scopus 로고    scopus 로고
    • Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli
    • Svensson, J., Andersson, C., Reseland, J.E., Lyngstadaas, P. & Bulow, L. Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli. Protein Expr. Purif. 48, 134-141 (2006).
    • (2006) Protein Expr. Purif , vol.48 , pp. 134-141
    • Svensson, J.1    Andersson, C.2    Reseland, J.E.3    Lyngstadaas, P.4    Bulow, L.5
  • 5
    • 0033810138 scopus 로고    scopus 로고
    • Carica papaya glutamine cyclotransferase belongs to a novel plant enzyme subfamily: Cloning and characterization of the recombinant enzyme
    • Dahl, S.W. et al. Carica papaya glutamine cyclotransferase belongs to a novel plant enzyme subfamily: Cloning and characterization of the recombinant enzyme. Protein Expr. Purif. 20, 27-36 (2000).
    • (2000) Protein Expr. Purif , vol.20 , pp. 27-36
    • Dahl, S.W.1
  • 6
    • 0023395492 scopus 로고
    • Influence of the codon following AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli
    • Looman, A.C. et al. Influence of the codon following AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. EMBO J. 6, 2489-2492 (1987).
    • (1987) EMBO J , vol.6 , pp. 2489-2492
    • Looman, A.C.1
  • 7
    • 0025341284 scopus 로고
    • In vivo processing of N-terminal methionine in E.coli
    • Dalbøge, H., Bayne, S. & Pedersen, J. In vivo processing of N-terminal methionine in E.coli. FEBS Lett. 266, 1-3 (1990).
    • (1990) FEBS Lett , vol.266 , pp. 1-3
    • Dalbøge, H.1    Bayne, S.2    Pedersen, J.3
  • 8
    • 34248679167 scopus 로고    scopus 로고
    • Tricine SDS-PAGE.
    • Schägger, H. Tricine SDS-PAGE. Nature Protocols 1, 16-22 (2006).
    • (2006) Nature Protocols , vol.1 , pp. 16-22
    • Schägger, H.1
  • 9
    • 33645471986 scopus 로고    scopus 로고
    • A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production
    • Cabrita, L.D., Dai, W. & Bottomley, S.P. A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnology 6, 12 (2006).
    • (2006) BMC Biotechnology , vol.6 , pp. 12
    • Cabrita, L.D.1    Dai, W.2    Bottomley, S.P.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.