Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: Facile production of protein building blocks for protein ligation

Gene

Volumn 231, Issue 1-2, 1999, Pages 1-13

  Mathys, Sibylle ^a   Evans Jr , Thomas C ^a   Chute, Ian C ^b   Wu, Hong ^b   Chong, Shaorong ^a   Benner, Jack ^a   Liu, Xiang Qin ^b   Xu, Ming Qun ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

Chitin binding domain; Escherichia coli expression vector; Protein purification; Protein splicing

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; CHITIN; INTEIN PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CYCLIZATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE MUTATION; GENETIC ENGINEERING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROCESSING; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ASPARAGINE; BACTERIAL PROTEINS; BASE SEQUENCE; CATALYSIS; CYANOBACTERIA; CYSTEINE; DNA HELICASES; DNA PRIMERS; DNAB HELICASES; HYDROLYSIS; MUTATION; RNA SPLICING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SYNECHOCYSTIS SP.;

EID: 0033614484     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00103-1     Document Type: Article

References (35)

1. Armstrong A., Brown R.S., Tsugita A. Primary structure and genetic organization of phage T4 DNA ligase. Nucleic Acids Res. 11:1983;7145-7156.
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
3. Chong S., Montello G.E., Zhang A., Cantor E.J., Liao W., Xu M.-Q., Benner J. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res. 26:1998;5109-5115.
4. Chong S., Williams K.S., Wotkowics C., Xu M.Q. Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J. Biol. Chem. 273:1998;10567-10577.
5. Chong S., Yang S., Paulus H., Benner J., Perler F.B., Xu M.-Q. Protein splicing involving the Saccharomyces cerevisiae VMA intein: the steps in the splicing pathway, side reactions leading to protein cleavage and establishment of an in vitro splicing system. J. Biol. Chem. 271:1996;22159-22168.
6. Chong S., Mersha F.B., Comb D.G., Scott M.E., Landry D., Vence L.M., Perler F.B., Benner J., Kucera R.B., Hirvonen C.A., Pelletier J.J., Paulus H., Xu M.-Q. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene. 192:1997;271-281.
7. Cooper A.A., Chen Y., Lindorfer M.A., Stevens T.H. Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision. EMBO J. 12:1993;2575-2583.
8. Davis E.O., Jenner P.J., Brooks P.C., Colston M.J., Sedgwick S.G. Protein splicing in the maturation of M. tuberculosis RecA protein: a mechanism for tolerating a novel class of intervening sequence. Cell. 71:1992;201-210.
9. Dawson P.E., Muir T.W., Clark-Lewis I., Kent S.B.H. Synthesis of proteins by native chemical ligation. Science. 266:1994;776-779.
10. Dubendorff J.W., Studier F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J. Mol. Biol. 219:1991;45-59.
11. Evans T.C. Jr., Benner J., Xu M.-Q. Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7:1998;2256-2264.
12. Evans T.C., Benner J., Xu M.-Q. The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J. Biol. Chem. 274:1999;3923-3926.
13. Gimble F.S. Putting protein splicing to work. Chem. Biol. 5:1998;R251-R256.
14. Guan C., Li P., Riggs P.D., Inouye H. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene. 67:1988;21-30.
15. +-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 265:1990;6726-6733.
16. +-adenosine triphosphatase. Science. 250:1990;651-657.
17. Klabunde T., Sharma S., Telenti A., Jacobs W.R. Jr., Sacchettini J.C. Crystal structure of gyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nature Struct. Biol. 5:1998;31-36.
18. Kunkel T.A., Roberts J.D., Zakour R.A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:1987;367-382.
19. LaVallie E.R., McCoy J.M. Gene fusion expression systems in Escherichia coli. Curr. Opin. Biotechnol. 6:1995;501-506.
20. Muir T.W., Sondhi D., Cole P.A. Expressed protein ligation: a general method for protein engineering. Proc. Natl. Acad. Sci. USA. 95:1998;6705-6710.
21. Perler F.B., Davis E.O., Dean G.E., Gimble F.S., Jack W.E., Neff N., Noren C.J., Thorner J., Belfort M. Protein splicing elements: inteins and exteins a definition of terms and recommended nomenclature. Nucleic Acids Res. 22:1994;1125-1127.
22. Perler F.B., Olsen G.J., Adam E. Compilation and analysis of intein sequences. Nucleic Acids Res. 25:1997;1087-1093.
23. Pietrokovski S. A new intein in Cyanobacteria and its significance for the spread of intein. Trends Genet. 12:1996;287-288.
24. Pietrokovski S. Modular organization of inteins and C-terminal autocatalytic domains. Protein Sci. 7:1998;64-71.
25. Severinov K., Muir T.W. Expressed protein ligation, a novel method for studying protein-protein interactions in transcription. J. Biol. Chem. 273:1998;16205-16209.
26. Shao Y., Xu M.-Q., Paulus H. Protein splicing: Evidence for an N-O acyl rearrangement as the initial step in the splicing process. Biochemistry. 35:1996;3810-3815.
27. Shao Y., Xu M.-Q., Paulus H. Protein splicing: estimation of the rate of O-N and S-N acyl rearrangement, the last step of the splicing process. J. Peptide Res. 50:1997;193-198.
28. Smith D.B., Johnson K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 67:1988;31-40.
29. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
30. Tam J.P., Lu Y.-A., Liu C.-F., Shao J. Peptide synthesis using unprotected peptides through orthogonal coupling methods. Proc. Natl. Acad. Sci. USA. 92:1995;12485-12489.
31. Watanabe T., Ito Y., Yamada T., Hashimoto M., Sekine S., Tanaka H. The role of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation. J. Bacteriol. 176:1994;4465-4472.
32. Wu H., Xu M.-Q., Liu X.-Q. Protein trans-splicing and functional mini-inteins of a cyanobacterial DnaB intein. Biochim. Biophys. Acta. 1387:1998;422-432.
33. Xu M.-Q., Southworth M.W., Mersha F.B., Hornstra L.J., Perler F.B. In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell. 75:1993;1371-1377.
34. Xu M.Q., Comb D.G., Paulus H., Noren C.J., Shao Y., Perler F.B. Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. EMBO J. 13:1994;5517-5522.
35. Xu M.-Q., Perler F.B. The mechanism of protein splicing and its modulation by mutation. EMBO J. 15:1996;5146-5153.