Rapid cloning and purification of proteins: Gateway vectors for protein purification by self-cleaving tags

Biotechnology and Bioengineering

Volumn 101, Issue 2, 2008, Pages 229-240

  Gillies, Alison R ^a   Hsii, Judy F ^a   Oak, Seachol ^a   Wood, David W ^a  

^a Princeton University   (United States)

Author keywords

Bioprocess optimization; Gateway cloning; High throughput screening; Intein based purification

Indexed keywords

BIOPROCESS OPTIMIZATION; GATEWAY CLONING; HIGH-THROUGHPUT SCREENING; INTEIN-BASED PURIFICATION;

CLONING; ESCHERICHIA COLI; GENETIC ENGINEERING; OPTIMIZATION; PLANTS (BOTANY); POLYPEPTIDES; PROTEINS; PURIFICATION; TARGETS; VECTORS;

TELECOMMUNICATION SYSTEMS;

BETA LACTAMASE; BUTYRIC ACID DERIVATIVE; CATALASE; CHITIN; INTEIN; MALTOSE BINDING PROTEIN; POLYHYDROXYBUTYRATE; POLYPEPTIDE; UNCLASSIFIED DRUG; BETA LACTAMASE TEM 1; BETA-LACTAMASE TEM-1; ESCHERICHIA COLI PROTEIN; KATG PROTEIN, E COLI; RECOMBINANT PROTEIN;

ANALYTIC METHOD; ARTICLE; CLONING VECTOR; ESCHERICHIA COLI; GENETIC ENGINEERING; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; PROCESS OPTIMIZATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; PLASMID;

ESCHERICHIA COLI;

BETA-LACTAMASES; CATALASE; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; INTEINS; PLASMIDS; RECOMBINANT PROTEINS;

EID: 51649125516     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21974     Document Type: Article

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