Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose

Journal of Chromatography A

Volumn 864, Issue 2, 1999, Pages 247-256

  Chaga, Grigoriy ^a   Bochkariov, Dmitry E ^a   Jokhadze, George G ^a   Hopp, Jennifer ^a   Nelson, Paul ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

Cobalt; Immobilized metal affinity chromatography; Peptides; Proteins

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; DIHYDROFOLATE REDUCTASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GENE CONSTRUCT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TECHNIQUE;

AFFINITY LABELS; AMINO ACID SEQUENCE; ASPARTIC ACID; BASE SEQUENCE; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CROSS-LINKING REAGENTS; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTIDINE; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SEPHAROSE; TETRAHYDROFOLATE DEHYDROGENASE;

ESCHERICHIA COLI;

EID: 0032757296     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(99)01008-0     Document Type: Article

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